1887

Abstract

There is a need for new compounds to effectively treat methicillin-resistant (MRSA) and vancomycin-resistant (VRE). The novel monobiguanide compound 1-(3,4-dichlorobenzyl)-5-octylbiguanide gluconate (OPB-2045G) has potential bactericidal activity. We sought to elucidate the potency of OPB-2045G bactericidal activity against MRSA and VRE compared to those of chlorhexidine digluconate (CHG) and povidone iodine (PVP-I). bactericidal activity was analysed using minimum bactericidal concentration (MBC) as the index. The bactericidal efficacy of OPB-2045G was examined by determining MRSA and VRE contamination of the normal dorsal skin of mice following removal of hair. After a 3 min treatment period, the MBC of OPB-2045G was lower than that of CHG and PVP-I against standard strains and clinical isolates. Additionally, in our mouse model, the bactericidal activity of 1.5 % OPB-2045G (a clinically relevant dose) was higher than that of 0.5 % CHG and equivalent to that of 10 % PVP-I against MRSA. Similarly, the bactericidal activity of OPB-2045G was higher than that of 0.5 % CHG and 10 % PVP-I against VRE. OPB-2045G showed more potent bactericidal activity against MRSA and VRE both and compared to CHG and PVP-I, indicating that OPB-2045G may provide better protection against health care-associated infections caused by these pathogens.

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2015-01-01
2020-09-21
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References

  1. ASTM 2013; E1174–13: Standard test method for evaluation of the effectiveness of health care personnel handwash formulations. In Annual Book of ASTM Standards vol. 11.05 West Conshohocken, PA: ASTM International;
    [Google Scholar]
  2. Backman C., Taylor G., Sales A., Marck P. B. 2011; An integrative review of infection prevention and control programs for multidrug-resistant organisms in acute care hospitals: a socio-ecological perspective. Am J Infect Control 39:368–378 [CrossRef][PubMed]
    [Google Scholar]
  3. Darouiche R. O., Wall M. J. Jr, Itani K. M. F., Otterson M. F., Webb A. L., Carrick M. M., Miller H. J., Awad S. S., Crosby C. T.& other authors ( 2010; Chlorhexidine-alcohol versus povidone-iodine for surgical-site antisepsis. N Engl J Med 362:18–26 [CrossRef][PubMed]
    [Google Scholar]
  4. De Angelis G., Cataldo M. A., De Waure C., Venturiello S., La Torre G., Cauda R., Carmeli Y., Tacconelli E. 2014; Infection control and prevention measures to reduce the spread of vancomycin-resistant enterococci in hospitalized patients: a systematic review and meta-analysis. J Antimicrob Chemother 69:1185–1192 [CrossRef][PubMed]
    [Google Scholar]
  5. Digison M. B. 2007; A review of anti-septic agents for pre-operative skin preparation. Plast Surg Nurs 27:185–189 [CrossRef][PubMed]
    [Google Scholar]
  6. Garnes A. L., Davidson E., Taylor L. E., Felix A. J., Shidlovsky B. A., Prigot A. 1959; Clinical evaluation of povidone-iodine aerosol spray in surgical practice. Am J Surg 97:49–53 [CrossRef][PubMed]
    [Google Scholar]
  7. Ito S. 2000; Drug therapy for breast-feeding women. N Engl J Med 343:118–126 [CrossRef][PubMed]
    [Google Scholar]
  8. Jones R. N. 2001; Resistance patterns among nosocomial pathogens: trends over the past few years. Chest 119:Suppl397S–404S [CrossRef][PubMed]
    [Google Scholar]
  9. Kampf G., Höfer M., Wendt C. 1999; Efficacy of hand disinfectants against vancomycin-resistant enterococci in vitro. J Hosp Infect 42:143–150 [CrossRef][PubMed]
    [Google Scholar]
  10. Kobayashi H., Tsuzuki M., Hosobuchi K. 1989; Bactericidal effects of antiseptics and disinfectants against methicillin-resistant Staphylococcus aureus. Infect Control Hosp Epidemiol 10:562–564 [CrossRef][PubMed]
    [Google Scholar]
  11. Kramer A., Wagenvoort H., Ahrén C., Daniels-Haardt I., Hartemann P., Kobayashi H., Kurcz A., Picazo J., Privitera G., Assadian O. 2010; Epidemiology of MRSA and current strategies in Europe and Japan. GMS Krankenhhyg Interdiszip 5:Doc01[PubMed]
    [Google Scholar]
  12. Magill S. S., Edwards J. R., Bamberg W., Beldavs Z. G., Dumyati G., Kainer M. A., Lynfield R., Maloney M., McAllister-Hollod L.& other authors ( 2014; Multistate point-prevalence survey of health care-associated infections. N Engl J Med 370:1198–1208 [CrossRef][PubMed]
    [Google Scholar]
  13. Maillard J. Y., Messager S., Veillon R. 1998; Antimicrobial efficacy of biocides tested on skin using an ex-vivo test. J Hosp Infect 40:313–323 [CrossRef][PubMed]
    [Google Scholar]
  14. Messager S., Goddard P. A., Dettmar P. W., Maillard J. Y. 2001; Determination of the antibacterial efficacy of several antiseptics tested on skin by an ‘ex-vivo’ test. J Med Microbiol 50:284–292[PubMed]
    [Google Scholar]
  15. Morioka I., Takahashi N., Kitajima H.Committee for Infection Prevention and Vaccine Promotion of the Japan Society for Premature and Newborn Medicine 2014; Prevalence of MRSA colonization in Japanese neonatal care unit patients in 2011. Pediatr Int 56:211–214 [CrossRef][PubMed]
    [Google Scholar]
  16. Oishi T., Iwata S., Nonoyama M., Tsuji A., Sunakawa K. 2004; Double-blind comparative study on the care of the neonatal umbilical cord using 80% ethanol with or without chlorhexidine. J Hosp Infect 58:34–37 [CrossRef][PubMed]
    [Google Scholar]
  17. Paulson D. S. 2003 Handbook of Topical Antimicrobials: Industrial Applications in Consumer Products and Pharmaceuticals New York: Marcel Dekker;
    [Google Scholar]
  18. Sakuragi T., Yanagisawa K., Dan K. 1995; Bactericidal activity of skin disinfectants on methicillin-resistant Staphylococcus aureus. Anesth Analg 81:555–558[PubMed]
    [Google Scholar]
  19. Sauerbrei A., Wutzler P. 2010; Virucidal efficacy of povidone-iodine-containing disinfectants. Lett Appl Microbiol 51:158–163[PubMed]
    [Google Scholar]
  20. Silvestri D. L., McEnery-Stonelake M. 2013; Chlorhexidine: uses and adverse reactions. Dermatitis 24:112–118 [CrossRef][PubMed]
    [Google Scholar]
  21. Simor A. E., Williams V., McGeer A., Raboud J., Larios O., Weiss K., Hirji Z., Laing F., Moore C., Gravel D.Community and Hospital Infection Control Association–Canada 2013; Prevalence of colonization and infection with methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus and of Clostridium difficile infection in Canadian hospitals. Infect Control Hosp Epidemiol 34:687–693 [CrossRef][PubMed]
    [Google Scholar]
  22. Tsubouchi H., Ohguro K., Yasumura K., Ishikawa H., Kikuchi M. 1997; Synthesis and structure-activity relationships of novel antiseptics. Bioorg Med Chem Lett 7:1721–1724 [CrossRef]
    [Google Scholar]
  23. Zamora J. L., Price M. F., Chuang P., Gentry L. O. 1985; Inhibition of povidone-iodine’s bactericidal activity by common organic substances: an experimental study. Surgery 98:25–29[PubMed]
    [Google Scholar]
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