1887

Abstract

species play an integral part in the health of the vaginal microbiota. We compared vaginal species in women from India and the USA with and without bacterial vaginosis (BV). Between July 2009 and November 2010, a cross-sectional study was conducted among 40 women attending a women’s health clinic in Mysore, India, and a sexually transmitted diseases clinic in San Francisco, USA. Women were diagnosed with BV using Amsel’s criteria and the Nugent score. 16S rDNA was sequenced to speciate the cultured isolates. Ten Indian and 10 US women without BV were compared with an equal number of women with BV. Lactobacilli were isolated from all healthy women, but from only 10 % of Indian and 50 % of US women with BV. 16S rDNA from 164 colonies was sequenced from healthy women (126 colonies) and women with BV (38 colonies). Seven cultivable species were isolated from 11 Indian women and nine species from 15 US women. The majority of species among Indian women were (25 .0%), (25.0 %) and (16.7 %). Among US women, (32.0 %), (20.0 %) and (12.0 %) predominated. and dominated the vaginal flora of healthy Indian and US women. Indian women appeared to have a higher percentage of obligate heterofermentative species, suggesting the need for a larger degree of metabolic flexibility and a more challenging vaginal environment.

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2014-07-01
2024-12-03
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References

  1. Amsel R., Totten P. A., Spiegel C. A., Chen K. C., Eschenbach D., Holmes K. K. 1983; Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. Am J Med 74:14–22 [View Article][PubMed]
    [Google Scholar]
  2. Antonio M. A., Hawes S. E., Hillier S. L. 1999; The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis 180:1950–1956 [View Article][PubMed]
    [Google Scholar]
  3. Antonio M. A., Rabe L. K., Hillier S. L. 2005; Colonization of the rectum by Lactobacillus species and decreased risk of bacterial vaginosis. J Infect Dis 192:394–398 [View Article][PubMed]
    [Google Scholar]
  4. Anukam K. C., Osazuwa E. O., Ahonkhai I., Reid G. 2006; Lactobacillus vaginal microbiota of women attending a reproductive health care service in Benin city, Nigeria. Sex Transm Dis 33:59–62 [View Article][PubMed]
    [Google Scholar]
  5. Bäckhed F., Ley R. E., Sonnenburg J. L., Peterson D. A., Gordon J. I. 2005; Host-bacterial mutualism in the human intestine. Science 307:1915–1920 [View Article][PubMed]
    [Google Scholar]
  6. Damelin L. H., Paximadis M., Mavri-Damelin D., Birkhead M., Lewis D. A., Tiemessen C. T. 2011; Identification of predominant culturable vaginal Lactobacillus species and associated bacteriophages from women with and without vaginal discharge syndrome in South Africa. J Med Microbiol 60:180–183 [View Article][PubMed]
    [Google Scholar]
  7. Felis G. E., Dellaglio F. 2007; Taxonomy of lactobacilli and bifidobacteria. Curr Issues Intest Microbiol 8:44–61[PubMed]
    [Google Scholar]
  8. Fredricks D. N., Fiedler T. L., Marrazzo J. M. 2005; Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 353:1899–1911 [View Article][PubMed]
    [Google Scholar]
  9. Garg K. B., Ganguli I., Das R., Talwar G. P. 2009; Spectrum of Lactobacillus species present in healthy vagina of Indian women. Indian J Med Res 129:652–657[PubMed]
    [Google Scholar]
  10. Giorgi A., Torriani S., Dellaglio F., Bo G., Stola E., Bernuzzi L. 1987; Identification of vaginal lactobacilli from asymptomatic women. Microbiologica 10:377–384[PubMed]
    [Google Scholar]
  11. Gonzalez A., Clemente J. C., Shade A., Metcalf J. L., Song S. J., Prithiviraj B., Palmer B. E., Knight R. 2011; Our microbial selves: what ecology can teach us. EMBO Rep 12:775–784 [View Article][PubMed]
    [Google Scholar]
  12. Jin L., Tao L., Pavlova S. I., So J. S., Kiwanuka N., Namukwaya Z., Saberbein B. A., Wawer M. 2007; Species diversity and relative abundance of vaginal lactic acid bacteria from women in Uganda and Korea. J Appl Microbiol 102:1107–1115[PubMed]
    [Google Scholar]
  13. Kazi Y. F., Saleem S., Kazi N. 2012; Investigation of vaginal microbiota in sexually active women using hormonal contraceptives in Pakistan. BMC Urol 12:22 [View Article][PubMed]
    [Google Scholar]
  14. Martín R., Soberón N., Vaneechoutte M., Flórez A. B., Vázquez F., Suárez J. E. 2008; Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates. Int Microbiol 11:261–266[PubMed]
    [Google Scholar]
  15. Martinez R. C., Franceschini S. A., Patta M. C., Quintana S. M., Nunes A. C., Moreira J. L., Anukam K. C., Reid G., De Martinis E. C. 2008; Analysis of vaginal lactobacilli from healthy and infected Brazilian women. Appl Environ Microbiol 74:4539–4542 [View Article][PubMed]
    [Google Scholar]
  16. Martinez-Freijo P., Fluit A. C., Schmitz F. J., Grek V. S., Verhoef J., Jones M. E. 1998; Class I integrons in Gram-negative isolates from different European hospitals and association with decreased susceptibility to multiple antibiotic compounds. J Antimicrob Chemother 42:689–696 [View Article][PubMed]
    [Google Scholar]
  17. Nugent R. P., Krohn M. A., Hillier S. L. 1991; Reliability of diagnosing bacterial vaginosis is improved by a standardized method of Gram stain interpretation. J Clin Microbiol 29:297–301[PubMed]
    [Google Scholar]
  18. Ocana V. S., Bru E., De Ruiz Holgado A. A., Nader-Macias M. E. 1999; Surface characteristics of lactobacilli isolated from human vagina. J Gen Appl Microbiol 45:203–212 [View Article][PubMed]
    [Google Scholar]
  19. Pavlova S. I., Kilic A. O., Kilic S. S., So J. S., Nader-Macias M. E., Simoes J. A., Tao L. 2002; Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences. J Appl Microbiol 92:451–459 [View Article][PubMed]
    [Google Scholar]
  20. Rantakokko-Jalava K., Jalava J. 2002; Optimal DNA isolation method for detection of bacteria in clinical specimens by broad-range PCR. J Clin Microbiol 40:4211–4217 [View Article][PubMed]
    [Google Scholar]
  21. Song Y. L., Kato N., Matsumiya Y., Liu C. X., Kato H., Watanabe K. 1999; Identification of and hydrogen peroxide production by fecal and vaginal lactobacilli isolated from Japanese women and newborn infants. J Clin Microbiol 37:3062–3064[PubMed]
    [Google Scholar]
  22. Thomas S. 1928; Döderlein’s bacillus: Lactobacillus acidophilus. J Infect Dis 43:218–227 [View Article]
    [Google Scholar]
  23. Vásquez A., Jakobsson T., Ahrné S., Forsum U., Molin G. 2002; Vaginal Lactobacillus flora of healthy Swedish women. J Clin Microbiol 40:2746–2749 [View Article][PubMed]
    [Google Scholar]
  24. Vásquez A., Molin G., Pettersson B., Antonsson M., Ahrné S. 2005; DNA-based classification and sequence heterogeneities in the 16S rRNA genes of Lactobacillus casei/paracasei and related species. Syst Appl Microbiol 28:430–441 [View Article][PubMed]
    [Google Scholar]
  25. Zhou X., Brown C. J., Abdo Z., Davis C. C., Hansmann M. A., Joyce P., Foster J. A., Forney L. J. 2007; Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women. ISME J 1:121–133 [View Article][PubMed]
    [Google Scholar]
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