1887

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Volume 63, Issue 7

Genetic characterization of spp. isolated from diarrhoeal and asymptomatic children Free

Abstract

Phenotypic and genetic characteristics of spp. isolated from diarrhoeal and asymptomatic children aged up to 5 years were analysed in this study. In total, 91 and 17 isolates were identified from diarrhoeal (case) and asymptomatic (control) children, respectively. All the isolates were tested for antimicrobial resistance, the presence of integrons, plasmid-mediated quinolone resistance (PMQR), virulence-associated genes and pathogenicity island (SH-PAI). The majority of the spp. from cases (68.1 %) and controls (82.3 %) were found to be resistant to fluoroquinolones. Integron carriage was detected more in cases (76.9 %) than in controls (35.5 %). Atypical class 1 integron was detected exclusively in from cases but not from the controls. PMQR genes such as and were detected in 82.4  and 14.3 % of the isolates from cases and in 53 and 17.6 % in controls, respectively. isolates from cases as well as from controls were positive for the invasive plasmid antigen H-encoding gene . The other virulence genes such as , , , , and were detected in isolates in 80.2, 49.4, 27.4, 27.4, 80.2 and 79.1 % of cases and in 64.7, 52.9, 17.6, 17.6, 64.7 and 64.7 % of controls, respectively. The entire SH-PAI was detected in serotype 2a from cases and controls. In an isolate from a control child, the SH-PAI was truncated. Integrons, PMQR and virulence-encoding genes were detected more frequently in cases than in controls. In diarrhoea endemic areas, asymptomatic carriers may play a crucial role in the transmission of multidrug-resistant spp. with all the putative virulence genes.

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© 2014 The Authors
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2014-07-01
2024-04-26
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Genetic characterization of Shigella spp. isolated from diarrhoeal and asymptomatic children
J. Med. Microbiol. 63, 903 (2014); https://doi.org/10.1099/jmm.0.070912-0
/content/journal/jmm/10.1099/jmm.0.070912-0
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