1887

Abstract

Phenotypic and genetic characteristics of spp. isolated from diarrhoeal and asymptomatic children aged up to 5 years were analysed in this study. In total, 91 and 17 isolates were identified from diarrhoeal (case) and asymptomatic (control) children, respectively. All the isolates were tested for antimicrobial resistance, the presence of integrons, plasmid-mediated quinolone resistance (PMQR), virulence-associated genes and pathogenicity island (SH-PAI). The majority of the spp. from cases (68.1 %) and controls (82.3 %) were found to be resistant to fluoroquinolones. Integron carriage was detected more in cases (76.9 %) than in controls (35.5 %). Atypical class 1 integron was detected exclusively in from cases but not from the controls. PMQR genes such as and were detected in 82.4  and 14.3 % of the isolates from cases and in 53 and 17.6 % in controls, respectively. isolates from cases as well as from controls were positive for the invasive plasmid antigen H-encoding gene . The other virulence genes such as , , , , and were detected in isolates in 80.2, 49.4, 27.4, 27.4, 80.2 and 79.1 % of cases and in 64.7, 52.9, 17.6, 17.6, 64.7 and 64.7 % of controls, respectively. The entire SH-PAI was detected in serotype 2a from cases and controls. In an isolate from a control child, the SH-PAI was truncated. Integrons, PMQR and virulence-encoding genes were detected more frequently in cases than in controls. In diarrhoea endemic areas, asymptomatic carriers may play a crucial role in the transmission of multidrug-resistant spp. with all the putative virulence genes.

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2014-07-01
2019-10-23
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References

  1. Afset J. E., Bruant G., Brousseau R., Harel J., Anderssen E., Bevanger L., Bergh K.. ( 2006;). Identification of virulence genes linked with diarrhea due to atypical enteropathogenic Escherichia coli by DNA microarray analysis and PCR. . J Clin Microbiol 44:, 3703–3711. [CrossRef][PubMed]
    [Google Scholar]
  2. Albert M. J., Faruque A. S., Faruque S. M., Sack R. B., Mahalanabis D.. ( 1999;). Case-control study of enteropathogens associated with childhood diarrhea in Dhaka, Bangladesh. . J Clin Microbiol 37:, 3458–3464.[PubMed]
    [Google Scholar]
  3. Bardhan P., Faruque A. S., Naheed A., Sack D. A.. ( 2010;). Decrease in shigellosis-related deaths without Shigella spp.-specific interventions, Asia. . Emerg Infect Dis 16:, 1718–1723. [CrossRef][PubMed]
    [Google Scholar]
  4. Behrens M., Sheikh J., Nataro J. P.. ( 2002;). Regulation of the overlapping pic/set locus in Shigella flexneri and enteroaggregative Escherichia coli. . Infect Immun 70:, 2915–2925. [CrossRef][PubMed]
    [Google Scholar]
  5. Bhattacharya D., Bhattacharjee H., Thamizhmani R., Sayi D. S., Bharadwaj A. P., Singhania M., Sugunan A. P., Roy S.. ( 2011;). Prevalence of the plasmid-mediated quinolone resistance determinants among clinical isolates of Shigella sp. in Andaman & Nicobar Islands, India. . Lett Appl Microbiol 53:, 247–251. [CrossRef][PubMed]
    [Google Scholar]
  6. Bodhidatta L., McDaniel P., Sornsakrin S., Srijan A., Serichantalergs O., Mason C. J.. ( 2010;). Case–control study of diarrheal disease etiology in a remote rural area in Western Thailand. . Am J Trop Med Hyg 83:, 1106–1109. [CrossRef][PubMed]
    [Google Scholar]
  7. Cavaco L. M., Aarestrup F. M.. ( 2009;). Evaluation of quinolones for use in detection of determinants of acquired quinolone resistance, including the new transmissible resistance mechanisms qnrA, qnrB, qnrS, and aac(6′)Ib-cr, in Escherichia coli and Salmonella enterica and determinations of wild-type distributions. . J Clin Microbiol 47:, 2751–2758. [CrossRef][PubMed]
    [Google Scholar]
  8. Cennimo D., Abbas A., Huang D. B., Chiang T.. ( 2009;). The prevalence and virulence characteristics of enteroaggregative Escherichia coli at an urgent-care clinic in the USA: a case–control study. . J Med Microbiol 58:, 403–407. [CrossRef][PubMed]
    [Google Scholar]
  9. Chang C. Y., Lu P. L., Lin C. C., Lee T. M., Tsai M. Y., Chang L. L.. ( 2011;). Integron types, gene cassettes, antimicrobial resistance genes and plasmids of Shigella sonnei isolates from outbreaks and sporadic cases in Taiwan. . J Med Microbiol 60:, 197–204. [CrossRef][PubMed]
    [Google Scholar]
  10. Chowdhury G., Pazhani G. P., Dutta D., Guin S., Dutta S., Ghosh S., Izumiya H., Asakura M., Yamasaki S.. & other authors ( 2012;). Vibrio fluvialis in patients with diarrhea, Kolkata, India. . Emerg Infect Dis 18:, 1868–1871. [CrossRef][PubMed]
    [Google Scholar]
  11. CLSI ( 2010;). Performance Standards for Antimicrobial Susceptibility Testing; 20th informational supplement M100-S20. Wayne, PA:: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  12. Frankel G., Giron J. A., Valmassoi J., Schoolnik G. K.. ( 1989;). Multi-gene amplification: simultaneous detection of three virulence genes in diarrhoeal stool. . Mol Microbiol 3:, 1729–1734. [CrossRef][PubMed]
    [Google Scholar]
  13. Garg P., Sinha S., Chakraborty R., Bhattacharya S. K., Nair G. B., Ramamurthy T., Takeda Y.. ( 2001;). Emergence of fluoroquinolone-resistant strains of Vibrio cholerae O1 biotype El Tor among hospitalized patients with cholera in Calcutta, India. . Antimicrob Agents Chemother 45:, 1605–1606. [CrossRef][PubMed]
    [Google Scholar]
  14. Gascón J., Vargas M., Schellenberg D., Urassa H., Casals C., Kahigwa E., Aponte J. J., Mshinda H., Vila J.. ( 2000;). Diarrhea in children under 5 years of age from Ifakara, Tanzania: a case–control study. . J Clin Microbiol 38:, 4459–4462.[PubMed]
    [Google Scholar]
  15. Gassama Sow A., Diallo M. H., Gatet M., Denis F., Aïdara-Kane A., Ploy M. C.. ( 2008;). Description of an unusual class 2 integron in Shigella sonnei isolates in Senegal (sub-Saharan Africa). . J Antimicrob Chemother 62:, 843–844. [CrossRef][PubMed]
    [Google Scholar]
  16. Ghosh S., Pazhani G. P., Chowdhury G., Guin S., Dutta S., Rajendran K., Bhattacharya M. K., Takeda Y., Niyogi S. K.. & other authors ( 2011;). Genetic characteristics and changing antimicrobial resistance among Shigella spp. isolated from hospitalized diarrhoeal patients in Kolkata, India. . J Med Microbiol 60:, 1460–1466. [CrossRef][PubMed]
    [Google Scholar]
  17. Hata M., Suzuki M., Matsumoto M., Takahashi M., Sato K., Ibe S., Sakae K.. ( 2005;). Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b. . Antimicrob Agents Chemother 49:, 801–803. [CrossRef][PubMed]
    [Google Scholar]
  18. Hien B. T. T., Trang D. T., Scheutz F., Cam P. D., Mølbak K., Dalsgaard A.. ( 2007;). Diarrhoeagenic Escherichia coli and other causes of childhood diarrhoea: a case–control study in children living in a wastewater-use area in Hanoi, Vietnam. . J Med Microbiol 56:, 1086–1096. [CrossRef][PubMed]
    [Google Scholar]
  19. Hien B. T., Scheutz F., Cam P. D., Serichantalergs O., Huong T. T., Thu T. M., Dalsgaard A.. ( 2008;). Diarrheagenic Escherichia coli and Shigella strains isolated from children in a hospital case–control study in Hanoi, Vietnam. . J Clin Microbiol 46:, 996–1004. [CrossRef][PubMed]
    [Google Scholar]
  20. Holt K. E., Baker S., Weill F. X., Holmes E. C., Kitchen A., Yu J., Sangal V., Brown D. J., Coia J. E.. & other authors ( 2012;). Shigella sonnei genome sequencing and phylogenetic analysis indicate recent global dissemination from Europe. . Nat Genet 44:, 1056–1059. [CrossRef][PubMed]
    [Google Scholar]
  21. Hong S., Choi Y. H., Choo Y. A., Choi Y., Choi S. Y., Kim D. W., Lee B. K., Park M. S.. ( 2010;). Genetic characterization of atypical Shigella flexneri isolated in Korea. . J Microbiol Biotechnol 20:, 1457–1462. [CrossRef][PubMed]
    [Google Scholar]
  22. Hossain M. A., Hasan K. Z., Albert M. J.. ( 1994;). Shigella carriers among non-diarrhoeal children in an endemic area of shigellosis in Bangladesh. . Trop Geogr Med 46:, 40–42.[PubMed]
    [Google Scholar]
  23. Hurt J. K., McQuade T. J., Emanuele A., Larsen M. J., Garcia G. A.. ( 2010;). High-throughput screening of the virulence regulator VirF: a novel antibacterial target for shigellosis. . J Biomol Screen 15:, 379–387. [CrossRef][PubMed]
    [Google Scholar]
  24. Ke X., Gu B., Pan S., Tong M.. ( 2011;). Epidemiology and molecular mechanism of integron-mediated antibiotic resistance in Shigella. . Arch Microbiol 193:, 767–774. [CrossRef][PubMed]
    [Google Scholar]
  25. Kim H. B., Park C. H., Kim C. J., Kim E. C., Jacoby G. A., Hooper D. C.. ( 2009;). Prevalence of plasmid-mediated quinolone resistance determinants over a 9-year period. . Antimicrob Agents Chemother 53:, 639–645. [CrossRef][PubMed]
    [Google Scholar]
  26. Lima I. F. N., Boisen N., da Quetz Silva J., Havt A., de Carvalho E. B., Soares A. M., Lima N. L., Mota R. M. S., Nataro J. P.. & other authors ( 2013;). Prevalence of enteroaggregative Escherichia coli and its virulence-related genes in a case–control study among children from north-eastern Brazil. . J Med Microbiol 62:, 683–693. [CrossRef][PubMed]
    [Google Scholar]
  27. Mammina C., Aleo A., Romani C., Nastasi A.. ( 2006;). Shigella sonnei biotype G carrying class 2 integrons in southern Italy: a retrospective typing study by pulsed field gel electrophoresis. . BMC Infect Dis 6:, 117. [CrossRef][PubMed]
    [Google Scholar]
  28. Niyogi S. K.. ( 2005;). Shigellosis. . J Microbiol 43:, 133–143.[PubMed]
    [Google Scholar]
  29. Noriega F. R., Liao F. M., Formal S. B., Fasano A., Levine M. M.. ( 1995;). Prevalence of Shigella enterotoxin 1 among Shigella clinical isolates of diverse serotypes. . J Infect Dis 172:, 1408–1410. [CrossRef][PubMed]
    [Google Scholar]
  30. Olusanya O., Shonukan O. O., Ogwo V. A.. ( 1990;). Childhood carriers of Salmonella and Shigella species in the rural area of Ile-Ife, Nigeria. . Ethiop Med J 28:, 49–52.[PubMed]
    [Google Scholar]
  31. Pan J. C., Ye R., Meng D. M., Zhang W., Wang H. Q., Liu K. Z.. ( 2006;). Molecular characteristics of class 1 and class 2 integrons and their relationships to antibiotic resistance in clinical isolates of Shigella sonnei and Shigella flexneri. . J Antimicrob Chemother 58:, 288–296. [CrossRef][PubMed]
    [Google Scholar]
  32. Panchalingam S., Antonio M., Hossain A., Mandomando I., Ochieng B., Oundo J., Ramamurthy T., Tamboura B., Zaidi A. K.. & other authors ( 2012;). Diagnostic microbiologic methods in the GEMS-1 case/control study. . Clin Infect Dis 55: (Suppl. 4), S294–S302. [CrossRef][PubMed]
    [Google Scholar]
  33. Parsot C.. ( 2005;). Shigella spp. and enteroinvasive Escherichia coli pathogenicity factors. . FEMS Microbiol Lett 252:, 11–18. [CrossRef][PubMed]
    [Google Scholar]
  34. Pazhani G. P., Niyogi S. K., Singh A. K., Sen B., Taneja N., Kundu M., Yamasaki S., Ramamurthy T.. ( 2008;). Molecular characterization of multidrug-resistant Shigella species isolated from epidemic and endemic cases of shigellosis in India. . J Med Microbiol 57:, 856–863. [CrossRef][PubMed]
    [Google Scholar]
  35. Phantouamath B., Sithivong N., Insisiengmay S., Ichinose Y., Higa N., Song T., Iwanaga M.. ( 2005;). Pathogenicity of Shigella in healthy carriers: a study in Vientiane, Lao People’s Democratic Republic. . Jpn J Infect Dis 58:, 232–234.[PubMed]
    [Google Scholar]
  36. Pu X. Y., Pan J. C., Wang H. Q., Zhang W., Huang Z. C., Gu Y. M.. ( 2009;). Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China. . J Antimicrob Chemother 63:, 917–920. [CrossRef][PubMed]
    [Google Scholar]
  37. Ranjbar R., Aleo A., Giammanco G. M., Dionisi A. M., Sadeghifard N., Mammina C.. ( 2007;). Genetic relatedness among isolates of Shigella sonnei carrying class 2 integrons in Tehran, Iran, 2002–2003. . BMC Infect Dis 7:, 62. [CrossRef][PubMed]
    [Google Scholar]
  38. Sepp E., Stsepetova J., Lõivukene K., Truusalu K., Kõljalg S., Naaber P., Mikelsaar M.. ( 2009;). The occurrence of antimicrobial resistance and class 1 integrons among commensal Escherichia coli isolates from infants and elderly persons. . Ann Clin Microbiol Antimicrob 8:, 34. [CrossRef][PubMed]
    [Google Scholar]
  39. Sinha S., Chattopadhyay S., Bhattacharya S. K., Nair G. B., Ramamurthy T.. ( 2004;). An unusually high level of quinolone resistance associated with type II topoisomerase mutations in quinolone resistance-determining regions of Aeromonas caviae isolated from diarrhoeal patients. . Res Microbiol 155:, 827–829. [CrossRef][PubMed]
    [Google Scholar]
  40. Sousa M. Â., Mendes E. N., Collares G. B., Péret-Filho L. A., Penna F. J., Magalhães P. P.. ( 2013;). Shigella in Brazilian children with acute diarrhoea: prevalence, antimicrobial resistance and virulence genes. . Mem Inst Oswaldo Cruz 108:, 30–35. [CrossRef][PubMed]
    [Google Scholar]
  41. Swierczewski B. E., Odundo E. A., Koech M. C., Ndonye J. N., Kirera R. K., Odhiambo C. P., Cheruiyot E. K., Wu M. T., Lee J. E.. & other authors ( 2013;). Surveillance for enteric pathogens in a case–control study of acute diarrhea in Western Kenya. . Trans R Soc Trop Med Hyg 107:, 83–90. [CrossRef][PubMed]
    [Google Scholar]
  42. Tajbakhsh M., García Migura L., Rahbar M., Svendsen C. A., Mohammadzadeh M., Zali M. R., Aarestrup F. M., Hendriksen R. S.. ( 2012;). Antimicrobial-resistant Shigella infections from Iran: an overlooked problem. ? J Antimicrob Chemother 67:, 1128–1133. [CrossRef][PubMed]
    [Google Scholar]
  43. Telli M., Guiral E., Martínez J. A., Almela M., Bosch J., Vila J., Soto S. M.. ( 2010;). Prevalence of enterotoxins among Escherichia coli isolates causing bacteraemia. . FEMS Microbiol Lett 306:, 117–121. [CrossRef][PubMed]
    [Google Scholar]
  44. Thong K. L., Hoe S. L., Puthucheary S. D., Yasin R. M.. ( 2005;). Detection of virulence genes in Malaysian Shigella species by multiplex PCR assay. . BMC Infect Dis 5:, 8. [CrossRef][PubMed]
    [Google Scholar]
  45. Torres A. G.. ( 2004;). Current aspects of Shigella pathogenesis. . Rev Latinoam Microbiol 46:, 89–97.[PubMed]
    [Google Scholar]
  46. Vargas M., Gascon J., Jimenez De Anta M. T., Vila J.. ( 1999;). Prevalence of Shigella enterotoxins 1 and 2 among Shigella strains isolated from patients with traveler’s diarrhea. . J Clin Microbiol 37:, 3608–3611.[PubMed]
    [Google Scholar]
  47. Xiong Z., Li J., Li T., Shen J., Hu F., Wang M.. ( 2010;). Prevalence of plasmid-mediated quinolone-resistance determinants in Shigella flexneri isolates from Anhui Province, China. . J Antibiot (Tokyo) 63:, 187–189. [CrossRef][PubMed]
    [Google Scholar]
  48. Yang H., Duan G., Zhu J., Lv R., Xi Y., Zhang W., Fan Q., Zhang M.. ( 2008;). The AcrAB-TolC pump is involved in multidrug resistance in clinical Shigella flexneri isolates. . Microb Drug Resist 14:, 245–249. [CrossRef][PubMed]
    [Google Scholar]
  49. Ye L. Y., Lan F. H., Zhu Z. Y., Chen X. M., Ye X. L.. ( 1993;). Detection of Shigella and enteroinvasive Escherichia coli using polymerase chain reaction. . J Diarrhoeal Dis Res 11:, 38–40.[PubMed]
    [Google Scholar]
  50. Zhang W., Luo Y., Li J., Lin L., Ma Y., Hu C., Jin S., Ran L., Cui S.. ( 2011;). Wide dissemination of multidrug-resistant Shigella isolates in China. . J Antimicrob Chemother 66:, 2527–2535. [CrossRef][PubMed]
    [Google Scholar]
  51. Zhang J., Qian L., Wu Y., Cai X., Li X., Cheng X., Qu D.. ( 2013;). Deletion of pic results in decreased virulence for a clinical isolate of Shigella flexneri 2a from China. . BMC Microbiol 13:, 31. [CrossRef][PubMed]
    [Google Scholar]
  52. Zhu J. Y., Duan G. C., Yang H. Y., Fan Q. T., Xi Y. L.. ( 2011;). Atypical class 1 integron coexists with class 1 and class 2 integrons in multi-drug resistant Shigella flexneri isolates from China. . Curr Microbiol 62:, 802–806. [CrossRef][PubMed]
    [Google Scholar]
  53. Zhu Y. L., Yang H. F., Liu Y. Y., Hu L. F., Cheng J., Ye Y., Li J. B.. ( 2013;). Detection of plasmid-mediated quinolone resistance determinants and the emergence of multidrug resistance in clinical isolates of Shigella in SiXian area, China. . Diagn Microbiol Infect Dis 75:, 327–329. [CrossRef][PubMed]
    [Google Scholar]
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