1887

Abstract

We performed a comparative molecular analysis on three types of clinically isolated spp.: epidemic sequence types (STs) of (epidemic ST-AB), non-epidemic sequence types of (non-epidemic ST-AB) and non- spp. A total of 87 isolates – 46 , 25 and 16 – from 43 hospitals were analysed. Of these, 31 isolates were ST1 or ST2 according to the Pasteur Institute multilocus sequence typing scheme and were defined as epidemic ST-AB. The other 15 isolates were defined as non-epidemic ST-AB. The epidemic ST-AB isolates harboured the gene or had an IS element upstream of , or both, whereas non-epidemic ST-AB and non- spp. isolates harboured or metallo-β-lactamase genes, or both. The proportion of multidrug-resistant isolates was significantly higher in the epidemic ST-AB isolates (48 %) than that in the other types of isolates (5 %) (<0.05). In addition, epidemic ST-AB isolates exhibited a relatively higher proportion of fluoroquinolone resistance. We demonstrated that, in terms of genotypes and phenotypes of antimicrobial resistance, non-epidemic ST-AB isolates shared more similarity with non- spp. isolates than with epidemic ST-AB isolates, regardless of bacterial species. In addition, this study revealed that, even in Japan, where IMP-type metallo-β-lactamase producers are endemic, epidemic ST-AB harbouring have not yet emerged.

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2014-06-01
2020-07-12
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