1887

Abstract

Animal models are essential for analysis of -related diseases. Mongolian gerbils are used frequently to study -induced gastritis and its consequences. The presence of some gastric microbiota with a suppressive effect on suggests inhibitory gastric bacteria against infection. The aim of the present study was to analyse the microbial ecology between and the gastric microbiota of Mongolian gerbils. Gastric mucosa samples of -negative and -positive gerbils were orally inoculated to five (Group 1) and six (Group 2) gerbils, respectively, and the gerbils were challenged with infection. The colonization rate (40 %) of in Group 1 gerbils was lower than the rate (67 %) in Group 2 gerbils. Culture filtrate of the gastric mucosa samples of Group 1 gerbils inhibited the growth of . Three lactobacilli species, , and , were isolated by anaerobic culture from the gerbils in Groups 1 and 2, and identified by genomic sequencing. It was demonstrated that the three different strains of lactobacilli exhibited an inhibitory effect on the growth of . The results suggested that lactobacilli are the dominant gastric microbiota of Mongolian gerbils and the three lactobacilli isolated from the gastric mucosa samples with an inhibitory effect on might have an anti-infective effect against .

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2014-01-01
2019-10-23
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References

  1. Atherton J. C. . ( 2006; ). The pathogenesis of Helicobacter pylori-induced gastro-duodenal diseases. . Annu Rev Pathol 1:, 63–96. [CrossRef] [PubMed]
    [Google Scholar]
  2. Aviles-Jimenez F. , Letley D. P. , Gonzalez-Valencia G. , Salama N. , Torres J. , Atherton J. C. . ( 2004; ). Evolution of the Helicobacter pylori vacuolating cytotoxin in a human stomach. . J Bacteriol 186:, 5182–5185. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bik E. M. , Eckburg P. B. , Gill S. R. , Nelson K. E. , Purdom E. A. , Francois F. , Perez-Perez G. , Blaser M. J. , Relman D. A. . ( 2006; ). Molecular analysis of the bacterial microbiota in the human stomach. . Proc Natl Acad Sci U S A 103:, 732–737. [CrossRef] [PubMed]
    [Google Scholar]
  4. Borchers A. T. , Selmi C. , Meyers F. J. , Keen C. L. , Gershwin M. E. . ( 2009; ). Probiotics and immunity. . J Gastroenterol 44:, 26–46. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chen X. , Liu X. M. , Tian F. , Zhang Q. , Zhang H. P. , Zhang H. , Chen W. . ( 2012; ). Antagonistic activities of lactobacilli against Helicobacter pylori growth and infection in human gastric epithelial cells. . J Food Sci 77:, M9–M14. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cross M. L. . ( 2002; ). Microbes versus microbes: immune signals generated by probiotic lactobacilli and their role in protection against microbial pathogens. . FEMS Immunol Med Microbiol 34:, 245–253. [CrossRef] [PubMed]
    [Google Scholar]
  7. DeLong E. F. . ( 1992; ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89:, 5685–5689. [CrossRef] [PubMed]
    [Google Scholar]
  8. Eaton K. A. , Morgan D. R. , Krakowka S. . ( 1992; ). Motility as a factor in the colonisation of gnotobiotic piglets by Helicobacter pylori . . J Med Microbiol 37:, 123–127. [CrossRef] [PubMed]
    [Google Scholar]
  9. Ferrero R. L. , Fox J. G. . ( 2001; ). In vivo modeling of Helicobacter-associated gastrointestinal diseases. . In Helicobacter pylori: Physiology and Genetics, pp. 565–582, Edited by Mobley H. L. T. , Mendz G. L. , Hazell S. L. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  10. Fukase K. , Kato M. , Kikuchi S. , Inoue K. , Uemura N. , Okamoto S. , Terao S. , Amagai K. , Hayashi S. , Asaka M. . Japan Gast Study Group ( 2008; ). Effect of eradication of Helicobacter pylori on incidence of metachronous gastric carcinoma after endoscopic resection of early gastric cancer: an open-label, randomised controlled trial. . Lancet 372:, 392–397. [CrossRef] [PubMed]
    [Google Scholar]
  11. Gill H. S. , Guarner F. . ( 2004; ). Probiotics and human health: a clinical perspective. . Postgrad Med J 80:, 516–526. [CrossRef] [PubMed]
    [Google Scholar]
  12. Gill H. S. , Rutherfurd K. J. , Prasad J. , Gopal P. K. . ( 2000; ). Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019). . Br J Nutr 83:, 167–176. [CrossRef] [PubMed]
    [Google Scholar]
  13. Gonchar N. V. , Berezina L. V. , Tikhomirova O. V. , Dobrolezh O. V. , Verbitskaia N. B. , Petrov L. N. , Bondarenko V. M. . ( 2009; ). [Choice of probiotic for rational therapy of infection caused by Klebsiella in children]. . Zh Mikrobiol Epidemiol Immunobiol 2:, 85–89.[PubMed]
    [Google Scholar]
  14. Guarner F. , Schaafsma G. J. . ( 1998; ). Probiotics. . Int J Food Microbiol 39:, 237–238. [CrossRef] [PubMed]
    [Google Scholar]
  15. Halper J. , Leshin L. S. , Lewis S. J. , Li W. I. . ( 2003; ). Wound healing and angiogenic properties of supernatants from Lactobacillus cultures. . Exp Biol Med (Maywood) 228:, 1329–1337.[PubMed]
    [Google Scholar]
  16. Hirayama F. , Takagi S. , Yokoyama Y. , Yamamoto K. , Iwao E. , Haga K. . ( 2002; ). Long-term effects of Helicobacter pylori eradication in Mongolian gerbils. . J Gastroenterol 37:, 779–784. [CrossRef] [PubMed]
    [Google Scholar]
  17. International Agency for Research on Cancer ( 1994; ). Schistosomes, Liver Flukes and Helicobacter pylori: IARC Monographs on the Evaluation of Carcinogenic Risk to Humans 61. Lyon:: IARC;.
    [Google Scholar]
  18. Ishida-Fujii K. , Sato R. , Goto S. , Yang X. P. , Kuboki H. , Hirano S. , Sato M. . ( 2007; ). Prevention of pathogenic Escherichia coli infection in mice and stimulation of macrophage activation in rats by an oral administration of probiotic Lactobacillus casei I-5. . Biosci Biotechnol Biochem 71:, 866–873. [CrossRef] [PubMed]
    [Google Scholar]
  19. Jain S. , Yadav H. , Sinha P. R. . ( 2009; ). Probiotic dahi containing Lactobacillus casei protects against Salmonella enteritidis infection and modulates immune response in mice. . J Med Food 12:, 576–583. [CrossRef] [PubMed]
    [Google Scholar]
  20. Johnson-Henry K. C. , Mitchell D. J. , Avitzur Y. , Galindo-Mata E. , Jones N. L. , Sherman P. M. . ( 2004; ). Probiotics reduce bacterial colonization and gastric inflammation in H. pylori-infected mice. . Dig Dis Sci 49:, 1095–1102. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kabir A. M. , Aiba Y. , Takagi A. , Kamiya S. , Miwa T. , Koga Y. . ( 1997; ). Prevention of Helicobacter pylori infection by lactobacilli in a gnotobiotic murine model. . Gut 41:, 49–55. [CrossRef] [PubMed]
    [Google Scholar]
  22. Kato-Mori Y. , Orihashi T. , Kanai Y. , Sato M. , Sera K. , Hagiwara K. . ( 2010; ). Fermentation metabolites from Lactobacillus gasseri and Propionibacterium freudenreichii exert bacteriocidal effects in mice. . J Med Food 13:, 1460–1467. [CrossRef] [PubMed]
    [Google Scholar]
  23. Kim M. N. , Kim N. , Lee S. H. , Park Y. S. , Hwang J. H. , Kim J. W. , Jeong S. H. , Lee D. H. , Kim J. S. . & other authors ( 2008; ). The effects of probiotics on PPI-triple therapy for Helicobacter pylori eradication. . Helicobacter 13:, 261–268. [CrossRef] [PubMed]
    [Google Scholar]
  24. Krakowka S. , Morgan D. R. , Kraft W. G. , Leunk R. D. . ( 1987; ). Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. . Infect Immun 55:, 2789–2796.[PubMed]
    [Google Scholar]
  25. Krausse R. , Piening K. , Ullmann U. . ( 2005; ). Inhibitory effects of various micro-organisms on the growth of Helicobacter pylori . . Lett Appl Microbiol 40:, 81–86. [CrossRef] [PubMed]
    [Google Scholar]
  26. Marchetti M. , Aricò B. , Burroni D. , Figura N. , Rappuoli R. , Ghiara P. . ( 1995; ). Development of a mouse model of Helicobacter pylori infection that mimics human disease. . Science 267:, 1655–1658. [CrossRef] [PubMed]
    [Google Scholar]
  27. Matsumoto S. , Washizuka Y. , Matsumoto Y. , Tawara S. , Ikeda F. , Yokota Y. , Karita M. . ( 1997; ). Induction of ulceration and severe gastritis in Mongolian gerbil by Helicobacter pylori infection. . J Med Microbiol 46:, 391–397. [CrossRef] [PubMed]
    [Google Scholar]
  28. Mégraud F. . ( 2004; ). H. pylori antibiotic resistance: prevalence, importance, and advances in testing. . Gut 53:, 1374–1384. [CrossRef] [PubMed]
    [Google Scholar]
  29. Michetti P. , Dorta G. , Wiesel P. H. , Brassart D. , Verdu E. , Herranz M. , Felley C. , Porta N. , Rouvet M. . & other authors ( 1999; ). Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans. . Digestion 60:, 203–209. [CrossRef] [PubMed]
    [Google Scholar]
  30. Mishra J. , Panigrahi S. . ( 2011; ). A study of changes in stomach wall at sites other than the ulcer in chronic duodenal ulcer patients. . Indian J Surg 73:, 262–263. [CrossRef] [PubMed]
    [Google Scholar]
  31. Nakagawa S. , Osaki T. , Fujioka Y. , Yamaguchi H. , Kamiya S. . ( 2005; ). Long-term infection of Mongolian gerbils with Helicobacter pylori: microbiological, histopathological, and serological analyses. . Clin Diagn Lab Immunol 12:, 347–353.[PubMed]
    [Google Scholar]
  32. Ohnishi N. . ( 1996; ). Re: Evaluation of new therapy for eradication of H. pylori infection in nude mouse model. . Am J Gastroenterol 91:, 174–175.[PubMed]
    [Google Scholar]
  33. Ojetti V. , Bruno G. , Ainora M. E. , Gigante G. , Rizzo G. , Roccarina D. , Gasbarrini A. . ( 2012; ). Impact of Lactobacillus reuteri supplementation on anti-Helicobacter pylori levofloxacin-based second-line therapy. . Gastroenterol Res Pract 2012:, 740381.[PubMed] [CrossRef]
    [Google Scholar]
  34. Osaki T. , Taguchi H. , Yamaguchi H. , Kamiya S. . ( 1998; ). Detection of Helicobacter pylori in fecal samples of gnotobiotic mice infected with H. pylori by an immunomagnetic-bead separation technique. . J Clin Microbiol 36:, 321–323.[PubMed]
    [Google Scholar]
  35. Osaki T. , Hanawa T. , Manzoku T. , Fukuda M. , Kawakami H. , Suzuki H. , Yamaguchi H. , Yan X. , Taguchi H. . & other authors ( 2006; ). Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model. . J Med Microbiol 55:, 1477–1485. [CrossRef] [PubMed]
    [Google Scholar]
  36. Osaki T. , Matsuki T. , Asahara T. , Zaman C. , Hanawa T. , Yonezawa H. , Kurata S. , Woo T. D. , Nomoto K. , Kamiya S. . ( 2012; ). Comparative analysis of gastric bacterial microbiota in Mongolian gerbils after long-term infection with Helicobacter pylori . . Microb Pathog 53:, 12–18. [CrossRef] [PubMed]
    [Google Scholar]
  37. Peek R. M. . ( 2008; ). Helicobacter pylori infection and disease: from humans to animal models. . Dis Model Mech 1:, 50–55. [CrossRef] [PubMed]
    [Google Scholar]
  38. Popova P. , Guencheva G. , Davidkova G. , Bogdanov A. , Pacelli E. , Opalchenova G. , Kutzarova T. , Koychev C. . ( 1993; ). Stimulating effect of DEODAN (an oral preparation from Lactobacillus bulgaricus “LB51”) on monocytes/macrophages and host resistance to experimental infections. . Int J Immunopharmacol 15:, 25–37. [CrossRef] [PubMed]
    [Google Scholar]
  39. Rinttilä T. , Kassinen A. , Malinen E. , Krogius L. , Palva A. . ( 2004; ). Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. . J Appl Microbiol 97:, 1166–1177. [CrossRef] [PubMed]
    [Google Scholar]
  40. Stackebrandt E. , Goodfellow M. . ( 1991; ). Nucleic Acid Techniques in Bacterial Systematics. Chichester:: Wiley;.
    [Google Scholar]
  41. Sun Y.-Q. , Monstein H.-J. , Nilsson L. E. , Petersson F. , Borch K. . ( 2003; ). Profiling and identification of eubacteria in the stomach of Mongolian gerbils with and without Helicobacter pylori infection. . Helicobacter 8:, 149–157. [CrossRef] [PubMed]
    [Google Scholar]
  42. Suzuki H. , Hibi T. , Marshall B. J. . ( 2007; ). Helicobacter pylori: present status and future prospects in Japan. . J Gastroenterol 42:, 1–15. [CrossRef] [PubMed]
    [Google Scholar]
  43. Tamura A. , Kumai H. , Nakamichi N. , Sugiyama T. , Deguchi R. , Takagi A. , Koga Y. . ( 2006; ). Suppression of Helicobacter pylori-induced interleukin-8 production in vitro and within the gastric mucosa by a live Lactobacillus strain. . J Gastroenterol Hepatol 21:, 1399–1406.[PubMed]
    [Google Scholar]
  44. Tatematsu M. , Tsukamoto T. , Toyoda T. . ( 2007; ). Effects of eradication of Helicobacter pylori on gastric carcinogenesis in experimental models. . J Gastroenterol 42: (Suppl 17), 7–9. [CrossRef] [PubMed]
    [Google Scholar]
  45. Tursi A. , Brandimarte G. , Giorgetti G. M. , Modeo M. E. . ( 2004; ). Effect of Lactobacillus casei supplementation on the effectiveness and tolerability of a new second-line 10-day quadruple therapy after failure of a first attempt to cure Helicobacter pylori infection. . Med Sci Monit 10:, CR662–CR666.[PubMed]
    [Google Scholar]
  46. Wang K. Y. , Li S. N. , Liu C. S. , Perng D. S. , Su Y. C. , Wu D. C. , Jan C. M. , Lai C. H. , Wang T. N. , Wang W. M. . ( 2004; ). Effects of ingesting Lactobacillus- and Bifidobacterium-containing yogurt in subjects with colonized Helicobacter pylori . . Am J Clin Nutr 80:, 737–741.[PubMed]
    [Google Scholar]
  47. Yamaguchi H. , Osaki T. , Taguchi H. , Sato N. , Toyoda A. , Takahashi M. , Kai M. , Nakata N. , Komatsu A. . & other authors ( 2003; ). Effect of bacterial flora on postimmunization gastritis following oral vaccination of mice with Helicobacter pylori heat shock protein 60. . Clin Diagn Lab Immunol 10:, 808–812.[PubMed]
    [Google Scholar]
  48. Zaman C. , Osaki T. , Hanawa T. , Yonezawa H. , Kurata S. , Kamiya S. . ( 2010; ). Analysis of the microflora in the stomach of Mongolian gerbils infected with Helicobacter pylori. . J Gastroenterol Hepatol 25: (Suppl 1), S11–S14. [CrossRef] [PubMed]
    [Google Scholar]
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