1887

Abstract

The region of the phage ϕCD27 was identified, located immediately downstream of the putative recombinase. The phage could integrate into two specific sites () in the genome, one of which was in an open reading frame encoding a putative ATPase of an ABC transporter and the other in an open reading frame encoding a putative ATPase of the flagella protein export apparatus. The prophage was capable of excision and formation of a circular molecule and phages were spontaneously released at a low frequency during growth. Infection and lysogeny of a strain previously shown to be sensitive to ϕCD27 were demonstrated, leading to a reduction in toxin production. Finally, a putative repressor was identified which is likely to be involved in maintaining lysogeny in these strains.

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2013-09-01
2019-10-19
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