1887

Abstract

strains JICH 05 and INCIENSA 04 were isolated from hospitalized leptospirosis patients in the province of Puntarenas, Costa Rica. The isolates produced agglutination titres notably against members of serogroups Pyrogenes and Tarassovi, respectively, but appeared serologically unique in the cross agglutinin absorption test (CAAT). Therefore, JICH 05 and INCIENSA 04 were considered to represent two new serovars, designated Corredores and Costa Rica of the serogroups Pyrogenes and Tarassovi, respectively. Multilocus sequence genotyping revealed that both strain INCIENSA 04 and strain JICH 05 belong to . These two new serovars are in addition to various other recently identified highly virulent serovars, including the new , serovar Arenal. Considering the fact that isolation and typing of leptospires from patients has only recently been introduced in Costa Rica, these findings suggest that various known and unknown virulent serovars of are circulating in this country and probably beyond, thus posing a severe threat to public and probably veterinary health in the region.

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2013-09-01
2020-01-25
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References

  1. Ahmed N. , Devi S. M. , Valverde M. de los A. , Vijayachari P. , Machang’u R. S. , Ellis W. A. , Hartskeerl R. A. . ( 2006; ). Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species. . Ann Clin Microbiol Antimicrob 5:, 28. [CrossRef] [PubMed]
    [Google Scholar]
  2. Ahmed A. , Thaipadungpanit J. , Boonsilp S. , Wuthiekanun V. , Nalam K. , Spratt B. G. , Aanensen D. M. , Smythe L. D. , Ahmed N. . & other authors ( 2011; ). Comparison of two multilocus sequence based genotyping schemes for Leptospira species. . PLoS Negl Trop Dis 5:, e1374. [CrossRef] [PubMed]
    [Google Scholar]
  3. Alex J. C. , Suresh B. , Korver H. , Ratnam S. , Venkatesan R. A. . ( 1993; ). Leptospira isolates of Madras city. . Indian J Med Microbiol 11:, 269–273.
    [Google Scholar]
  4. Anonymous . ( 1984; ). International committee on systematic bacteriology, subcommittee on the taxonomy of Leptospira: minutes of the meeting, 6 to 10 August 1982, Boston, MA, USA. . Int J Syst Bacteriol 34:, 258–259. [CrossRef]
    [Google Scholar]
  5. Anonymous . ( 1987; ). International committee on systematic bacteriology, subcommittee on the taxonomy of Leptospira: minutes of the meeting, 5 and 6 September 1986, Manchester, England. . Int J Syst Bacteriol 37:, 472–473. [CrossRef]
    [Google Scholar]
  6. Anonymous . ( 1994; ). Comisión Científica Permanente sobre Leptospirosis de la A. A. V. L. D. Manual de leptospirosis. Buenos Aires, Argentina:: Asociación Argentina de Veterinarios de Laboratorios de Diagnostico;.
    [Google Scholar]
  7. Brenner D. J. , Kaufmann A. F. , Sulzer K. R. , Steigerwalt A. G. , Rogers F. C. , Weyant R. S. . ( 1999; ). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. . Int J Syst Bacteriol 49:, 839–858. [CrossRef] [PubMed]
    [Google Scholar]
  8. Costa F. , Martinez-Silveira M. S. , Hagan J. E. , Hartskeerl R. A. , Dos Reis M. G. , Ko A. I. . ( 2012; ). Surveillance for leptospirosis in the Americas, 1996-2005: a review of data from ministries of health. . Rev Panam Salud Publica 32:, 169–177. [CrossRef] [PubMed]
    [Google Scholar]
  9. Dikken H. , Kmety E. . ( 1978; ). Serological typing methods of leptospires. . In Methods in Microbiology, vol. 11, pp. 259–307. Edited by Bergen T. , Norris J.R. . . New York:: Academic Press;.
    [Google Scholar]
  10. Faine S. . (editor) ( 1982; ). Guidelines for the Control of Leptospirosis. World Health Organization Offset Publication no. 67. . Geneva:: World Health Organization;.
  11. Faine S. . ( 1994; ). Leptospira and Leptospirosis. Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  12. Faine S. , Stallman N. . ( 1982; ). Amended descriptions of the genus Leptospira Noguchi 1917 and the species L. interrogans (Stimson 1907) Wenyon 1926 and L. biflexa (Wolbach and Binger 1914) Noguchi 1918. . IJSEM 32:, 461–463.
    [Google Scholar]
  13. Faine S. , Adler B. , Bolin C. , Perolat P. . ( 1999; ). Leptospira and Leptospirosis, 2nd edn . Armadale, Australia:: Medi sci Writing;.
    [Google Scholar]
  14. Goris M. G. A. , Leeflang M. M. G. , Boer K. R. , Goeijenbier M. , Van Gorp E. C. M. , Wagenaar J. F. P. , Hartskeerl R. A. . ( 2012; ). Establishment of valid laboratory case definition for human leptospirosis. . J Bacteriol Parasitol 3:, 132.[CrossRef]
    [Google Scholar]
  15. Gravekamp C. , Van de Kemp H. , Franzen M. , Carrington D. , Schoone G. J. , Van Eys G. J. , Everard C. O. , Hartskeerl R. A. , Terpstra W. J. . ( 1993; ). Detection of seven species of pathogenic leptospires by PCR using two sets of primers. . J Gen Microbiol 139:, 1691–1700. [CrossRef] [PubMed]
    [Google Scholar]
  16. Hartskeerl R. A. , Smits H. L. , Korver H. H. , Goris M. G. A. , Terpstra W. J. . ( 2006; ). International Course on Laboratory Methods for the Diagnosis of Leptospirosis, Course Manual. . Amsterdam, The Netherlands:: Royal Tropical Institute;.
  17. Kmety E. , Dikken H. . ( 1993; ). Classification of the Species Leptospira Interrogans and History of its Serovars. Groningen, The Netherlands:: University Press;.
    [Google Scholar]
  18. Korver H. , Kolk A. H. J. , Vingerhoed J. , van Leeuwen J. , Terpstra W. J. . ( 1988; ). Classification of the serovars of Icterohaemorrhagiae serogroup by monoclonal antibodies. . Isr J Vet Med 44:, 15–18.
    [Google Scholar]
  19. Levett P. N. . ( 2001; ). Leptospirosis. . Clin Microbiol Rev 14:, 296–326. [CrossRef] [PubMed]
    [Google Scholar]
  20. Levett P. N. . ( 2003; ). Leptospira and Leptonema. . In Manual of Clinical Microbiology, pp. 929–936. Edited by Murray P. R. , Baron E. J. , Jorgensen J. H. , Pfaller M. A. , Yolken R. H. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Levett P. N. , Morey R. E. , Galloway R. L. , Steigerwalt A. G. . ( 2006; ). Leptospira broomii sp. nov., isolated from humans with leptospirosis. . Int J Syst Evol Microbiol 56:, 671–673. [CrossRef] [PubMed]
    [Google Scholar]
  22. Limmathurotsakul D. , Turner E. L. , Wuthiekanun V. , Thaipadungpanit J. , Suputtamongkol Y. , Chierakul W. , Smythe L. D. , Day N. P. , Cooper B. , Peacock S. J. . ( 2012; ). Fool’s gold: Why imperfect reference tests are undermining the evaluation of novel diagnostics: a re-evaluation of five diagnostic tests for leptospirosis. . Clin Infect Dis 55:, 322–331. [CrossRef] [PubMed]
    [Google Scholar]
  23. Perolat P. , Chappel R. J. , Adler B. , Baranton G. , Bulach D. M. , Billinghurst M. L. , Letocart M. , Merien F. , Serrano M. S. . ( 1998; ). Leptospira fainei sp. nov., isolated from pigs in Australia. . Int J Syst Bacteriol 48:, 851–858. [CrossRef] [PubMed]
    [Google Scholar]
  24. Smits H. L. , van der Hoorn M. A. , Goris M. G. , Gussenhoven G. C. , Yersin C. , Sasaki D. M. , Terpstra W. J. , Hartskeerl R. A. . ( 2000; ). Simple latex agglutination assay for rapid serodiagnosis of human leptospirosis. . J Clin Microbiol 38:, 1272–1275.[PubMed]
    [Google Scholar]
  25. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  26. Valverde Ma. de los A. , Ramírez J. M. , Montes de Oca L. G. , Goris M. G. A. , Ahmed N. , Hartskeerl R. A. . ( 2008; ). Arenal, a new Leptospira serovar of serogroup Javanica, isolated from a patient in Costa Rica. . Infect Genet Evol 8:, 529–533. [CrossRef] [PubMed]
    [Google Scholar]
  27. Victoria B. , Ahmed A. , Zuerner R. L. , Ahmed N. , Bulach D. M. , Quinteiro J. , Hartskeerl R. A. . ( 2008; ). Conservation of the S10-spc-alpha locus within otherwise highly plastic genomes provides phylogenetic insight into the genus Leptospira . . PLoS ONE 3:, e2752. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yasuda P. H. , Steigenwalt A. G. , Sulzer K. R. , Kaufmann A. F. , Rogers F. , Brenner D. J. . ( 1987; ). Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. . Int J Syst Evol Microbiol 37:, 407–415.
    [Google Scholar]
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