1887

Abstract

Carbapenem-resistant is becoming increasingly prevalent in patients with diabetes mellitus in the Middle East. We examined the relationship of these bacteria and their resistance mechanisms to the diabetic disease status of patients in Saudi Arabia. Susceptibilities of 271 isolates to carbapenems, tigecycline and colistin were determined, followed by detection of carbapenemase genes. A gene was detected in ~95 % of isolates; and genes were also prevalent. Diabetic patients were significantly more likely to carry carbapenem-resistant isolates. Carbapenem-resistant is a serious problem in diabetic patients, and molecular detection of resistance mechanisms in these isolates is required.

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2013-06-01
2024-12-03
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References

  1. Al Johani S. M., Akhter J., Balkhy H., El-Saed A., Younan M., Memish Z. 2010; Prevalence of antimicrobial resistance among gram-negative isolates in an adult intensive care unit at a tertiary care center in Saudi Arabia. Ann Saudi Med 30:364–369[PubMed]
    [Google Scholar]
  2. Alsultan A. A., Hamouda A., Evans B. A., Amyes S. G. 2009; Acinetobacter baumannii: emergence of four strains with novel bla OXA-51-like genes in patients with diabetes mellitus. J Chemother 21:290–295[PubMed] [CrossRef]
    [Google Scholar]
  3. Andrews J. M. 2010; BSAC methods for antimicrobial susceptibility testing, version 9.1.
  4. Benjamini Y., Hochberg Y. 1995; Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc, B 57:289–300
    [Google Scholar]
  5. Boucher H. W., Talbot G. H., Bradley J. S., Edwards J. E., Gilbert D., Rice L. B., Scheld M., Spellberg B., Bartlett J. 2009; Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clin Infect Dis 48:1–12 [View Article][PubMed]
    [Google Scholar]
  6. Danaei G., Finucane M. M., Lu Y., Singh G. M., Cowan M. J., Paciorek C. J., Lin J. K., Farzadfar F., Khang Y. H. other authors 2011; National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2.7 million participants. Lancet 378:31–40 [View Article][PubMed]
    [Google Scholar]
  7. Ellington M. J., Kistler J., Livermore D. M., Woodford N. 2007; Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases. J Antimicrob Chemother 59:321–322 [View Article][PubMed]
    [Google Scholar]
  8. Evans B. A., Hamouda A., Amyes S. G. 2013; The rise of carbapenem-resistant Acinetobacter baumannii . Curr Pharm Des 19:223–238 [View Article][PubMed]
    [Google Scholar]
  9. Franklin C., Liolios L., Peleg A. Y. 2006; Phenotypic detection of carbapenem-susceptible metallo-beta-lactamase-producing gram-negative bacilli in the clinical laboratory. J Clin Microbiol 44:3139–3144 [View Article][PubMed]
    [Google Scholar]
  10. Higgins P. G., Dammhayn C., Hackel M., Seifert H. 2010; Global spread of carbapenem-resistant Acinetobacter baumannii . J Antimicrob Chemother 65:233–238 [View Article][PubMed]
    [Google Scholar]
  11. Metan G., Sariguzel F., Sumerkan B. 2009; Factors influencing survival in patients with multi-drug-resistant Acinetobacter bacteraemia. Eur J Intern Med 20:540–544 [View Article][PubMed]
    [Google Scholar]
  12. Michalopoulos A., Falagas M. E., Karatza D. C., Alexandropoulou P., Papadakis E., Gregorakos L., Chalevelakis G., Pappas G. 2011; Epidemiologic, clinical characteristics, and risk factors for adverse outcome in multiresistant gram-negative primary bacteremia of critically ill patients. Am J Infect Control 39:396–400 [View Article][PubMed]
    [Google Scholar]
  13. Mugnier P. D., Bindayna K. M., Poirel L., Nordmann P. 2009; Diversity of plasmid-mediated carbapenem-hydrolysing oxacillinases among carbapenem-resistant Acinetobacter baumannii isolates from Kingdom of Bahrain. J Antimicrob Chemother 63:1071–1073 [View Article][PubMed]
    [Google Scholar]
  14. Peleg A. Y., Franklin C., Bell J. M., Spelman D. W. 2005; Dissemination of the metallo-beta-lactamase gene blaIMP-4 among gram-negative pathogens in a clinical setting in Australia. Clin Infect Dis 41:1549–1556 [View Article][PubMed]
    [Google Scholar]
  15. Poirel L., Nordmann P. 2006; Genetic structures at the origin of acquisition and expression of the carbapenem-hydrolyzing oxacillinase gene bla OXA-58 in Acinetobacter baumannii . Antimicrob Agents Chemother 50:1442–1448 [View Article][PubMed]
    [Google Scholar]
  16. Prata-Rocha M. L., Gontijo-Filho P. P., Melo G. B. 2012; Factors influencing survival in patients with multidrug-resistant Acinetobacter baumannii infection. Braz J Infect Dis 16:237–241[PubMed]
    [Google Scholar]
  17. Towner K. J., Levi K., Vlassiadi M. ARPAC Steering Group 2008; Genetic diversity of carbapenem-resistant isolates of Acinetobacter baumannii in Europe. Clin Microbiol Infect 14:161–167 [View Article][PubMed]
    [Google Scholar]
  18. Turton J. F., Ward M. E., Woodford N., Kaufmann M. E., Pike R., Livermore D. M., Pitt T. L. 2006; The role of IS Aba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii . FEMS Microbiol Lett 258:72–77 [View Article][PubMed]
    [Google Scholar]
  19. Turton J. F., Gabriel S. N., Valderrey C., Kaufmann M. E., Pitt T. L. 2007; Use of sequence-based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumannii . Clin Microbiol Infect 13:807–815 [View Article][PubMed]
    [Google Scholar]
  20. Woodford N., Ellington M. J., Coelho J. M., Turton J. F., Ward M. E., Brown S., Amyes S. G. B., Livermore D. M. 2006; Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents 27:351–353 [View Article][PubMed]
    [Google Scholar]
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