1887

Abstract

One hundred and six nalidixic acid-resistant isolates from two Brazilian hospitals isolated from June to October 2010 were evaluated to characterize the co-existence of plasmid-mediated quinolone resistant (PMQR) and extended-spectrum β-lactamase (ESBL) determinants. The genetic environment was determined by PCR and sequencing. Conjugation and hybridization experiments determined whether -carrying plasmids were self-transferable. The and genes were also screened. Thirteen like genes (12.3 %) were identified, with the most common, followed by , and . No , or determinant was detected. All -positive strains possessed chromosomal substitutions in gyrase- and topoisomerase-encoding genes and four harboured a gene. The co-production of was observed in ten -positive strains. These results indicate the dissemination of PMQR genes shown in clinical isolates from Brazil, and their co-existence with ESBL genes emphasizes the complexity of plasmid-mediated resistance determinants among .

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2013-09-01
2020-01-21
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