1887

Abstract

Previous studies have implicated CagA [encoded by cytotoxin-associated gene A ()] in -associated gastroduodenal pathology and distinct subgenotypes of may circulate in different pathological manifestations of -positive infection. To investigate genotype and variants in Chinese strains and explore their relationship with gastroduodenal diseases, the status of 82 Chinese strains was examined and variation in size of the 3′ region of in 71 of these strains was analysed by PCR. was detected in 28 (100 %) of 28 strains from peptic ulcer patients, two (100 %) of two strains from gastric cancer patients, 32 (94.1 %) of 34 strains from chronic gastritis patients and 17 (94.4 %) of 18 strains from healthy volunteers. PCR products of the 3′ variable region were obtained from 71 (92.2 %) of 77 Chinese strains and could be classified into subgenotypes I, II and III, which gave PCR products of around 825, 900 and 950 bp, respectively. Subgenotype I predominated in Chinese strains (67/71), whereas subgenotype II presented in two isolates from patients with chronic gastritis and subgenotype III presented in two isolates from healthy volunteers. Therefore, neither nor its 3′ region variants can be used as a sole marker for the presence of particular -related gastroduodenal diseases in the Chinese population.

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2004-03-01
2019-11-12
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