1887

Abstract

This study investigated 147 multidrug-resistant and isolates from hospitalized patients in Malaysia. Class 1 integrons were the most dominant class identified (45.6 %). Three isolates were shown to contain class 2 integrons (2.0 %), whilst one isolate harboured both class 1 and 2 integrons. No class 3 integrons were detected in this study. In addition, the gene was amplified in 35 % of isolates and was significantly associated with the presence of integrase genes in an integron structure. RFLP and DNA sequencing analyses revealed the presence of 19 different cassette arrays among the detected integrons. The most common gene cassettes were those encoding resistance towards aminoglycosides () and trimethoprim (). As far as is known, this study is the first to identify integron-carrying cassette arrays such as , and in the Malaysian population. Patients’ age was demonstrated as a significant risk factor for the acquisition of integrons ( = 0.028). Epidemiological typing using PFGE also demonstrated a clonal relationship among isolates carrying identical gene cassettes in and but not in isolates.

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2013-03-01
2019-12-11
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