1887

Abstract

Various species have been isolated from the stomach, intestinal tract and liver of a variety of mammalian and some avian species, and DNA has been detected in human bile and liver samples. An immunoblot assay was established to analyse serum antibody responses to non-gastric species in patients with autoimmune liver diseases, in comparison with healthy individuals. Sera from 36 patients with primary sclerosing cholangitis (PSC), 21 with primary biliary cirrhosis, 19 with autoimmune chronic hepatitis and 80 blood donors were analysed by immunoblot, using cell-surface proteins from , and as antigens. Prior to testing, sera were cross-absorbed with a whole-cell lysate of . Antibody reactivity to various proteins of these three species was measured by densitometric scanning and results were processed by computer software to estimate antigenic specificity. Results were also compared with antibody response to . For , reactivity to at least two of the proteins with molecular masses of 48, 45, 37, 20 and 16 kDa, for , reactivity to the 76, 30 and 21 kDa proteins and for , reactivity to the 22 and 20 kDa proteins, seemed to have high specificity. Positive immunoblot results with sera from patients with PSC to antigens of , and were found in 38, 22 and 25 % of cases, respectively, and from patients with other autoimmune liver diseases, in 30, 22 and 22 % of cases, respectively. Prevalence of serum antibodies to non-gastric species was significantly higher in patients with autoimmune chronic liver diseases than in healthy blood donors ( < 0.001). Increased antibody levels to enterohepatic species raise questions concerning an infectious role of these emerging bacterial pathogens in human autoimmune liver diseases.

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2003-11-01
2019-11-13
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References

  1. Alvarez, F., Berg, P. A., Bianchi, F. B. & 39 other authors ( 1999;). International Autoimmune Hepatitis Group Report: review of criteria for diagnosis of autoimmune hepatitis. J Hepatol 31, 929–938.[CrossRef]
    [Google Scholar]
  2. Ananieva, O., Nilsson, I., Vorobjova, T., Uibo, R. & Wadström, T. ( 2002;). Immune responses to bile-tolerant Helicobacter species in patients with chronic liver diseases, a randomized population group, and healthy blood donors. Clin Diagn Lab Immunol 9, 1160–1164.
    [Google Scholar]
  3. Avenaud, P., Marais, A., Monteiro, L., Le Bail, B., Bioulac Sage, P., Balabaud, C. & Mégraud, F. ( 2000;). Detection of Helicobacter species in the liver of patients with and without primary liver carcinoma. Cancer 89, 1431–1439.[CrossRef]
    [Google Scholar]
  4. de Magalhaes Queiroz, D. M. & Santos, A. ( 2001;). Isolation of a Helicobacter strain from the human liver. Gastroenterology 121, 1023–1024.
    [Google Scholar]
  5. Fox, J. G., Dewhirst, F. E., Shen, Z. & 8 other authors ( 1998;). Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology 114, 755–763.[CrossRef]
    [Google Scholar]
  6. Fox, J. G., Schauer, D. B. & Wadström, T. ( 2001;). Enterohepatic Helicobacter species. Curr Opin Gastroenterol 17 (Suppl. 1), S28–S31.
    [Google Scholar]
  7. Garcia-Tsao, G. ( 2001;). Bacterial translocation: cause or consequence of decompensation in cirrhosis? J Hepatol 34, 150–155.
    [Google Scholar]
  8. Haydon, G. H. & Neuberger, J. ( 2000;). PBC: an infectious disease? Gut 47, 586–588.[CrossRef]
    [Google Scholar]
  9. Kornilovs'ka, I., Nilsson, I., Utt, M., Ljungh, Å. & Wadström, T. ( 2002;). Immunogenic proteins of Helicobacter pullorum, Helicobacter bilis and Helicobacter hepaticus identified by two-dimensional gel electrophoresis and immunoblotting. Proteomics 2, 775–783.[CrossRef]
    [Google Scholar]
  10. Laemmli, U. K. ( 1970;). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  11. Lee, Y.-M. & Kaplan, M. M. ( 1995;). Primary sclerosing cholangitis. N Engl J Med 332, 924–933.[CrossRef]
    [Google Scholar]
  12. Lelwala-Guruge, J., Nilsson, I., Ljungh, Å. & Wadström, T. ( 1992;). Cell surface proteins of Helicobacter pylori as antigens in an ELISA and a comparison with three commercial ELISA. Scand J Infect Dis 24, 457–465.[CrossRef]
    [Google Scholar]
  13. Leong, R. W. L. & Sung, J. J. Y. ( 2002;). Helicobacter species and hepatobiliary diseases. Aliment Pharmacol Ther 16, 1037–1045.[CrossRef]
    [Google Scholar]
  14. Ljungh, Å. & Wadström, T. ( 2002;). The role of microorganisms in biliary tract disease. Curr Gastroenterol Rep 4, 167–171.[CrossRef]
    [Google Scholar]
  15. Ljungh, Å., Lan, J. & Yanagisawa, N. ( 2002;). Isolation, selection and characteristics of Lactobacillus paracasei subsp.paracasei F19. Microb Ecol Health Dis 14 (Suppl. 3), 4–6.
    [Google Scholar]
  16. Matsukura, N., Yokomuro, S., Yamada, S., Tajiri, T., Sundo, T., Hadama, T., Kamiya, S., Naito, Z. & Fox, J. G. ( 2002;). Association between Helicobacter bilis in bile and biliary tract malignancies: H.bilis in bile from Japanese and Thai patients with benign and malignant diseases in the biliary tract. Jpn J Cancer Res 93, 842–847.[CrossRef]
    [Google Scholar]
  17. Nilsson, I., Ljungh, Å., Aleljung, P. & Wadström, T. ( 1997;). Immunoblot assay for serodiagnosis of Helicobacter pylori infections. J Clin Microbiol 35, 427–432.
    [Google Scholar]
  18. Nilsson, H.-O., Taneera, J., Castedal, M., Glatz, E., Olsson, R. & Wadström, T. ( 2000a;). Identification of Helicobacter pylori and other Helicobacter species by PCR, hybridization, and partial DNA sequencing in human liver samples from patients with primary sclerosing cholangitis or primary biliary cirrhosis. J Clin Microbiol 38, 1072–1076.
    [Google Scholar]
  19. Nilsson, I., Lindgren, S., Eriksson, S. & Wadström, T. ( 2000b;). Serum antibodies to Helicobacter hepaticus and Helicobacter pylori in patients with chronic liver disease. Gut 46, 410–414.[CrossRef]
    [Google Scholar]
  20. Nilsson, H.-O., Mulchandani, R., Tranberg, K.-G., Stenram, U. & Wadström, T. ( 2001;). Helicobacter species identified in liver from patients with cholangiocarcinoma and hepatocellular carcinoma. Gastroenterology 120, 323–324.[CrossRef]
    [Google Scholar]
  21. Nilsson, H.-O., Stenram, U., Ihse, I. & Wadström, T. ( 2002;). Re: Helicobacter pylori seropositivity as a risk factor for pancreatic cancer. J Natl Cancer Inst 94, 632–633.[CrossRef]
    [Google Scholar]
  22. Roe, I. H., Kim, J. T., Lee, H. S., Lee, J. H. ( 1999;). Detection of Helicobacter DNA in bile from bile duct diseases. J Korean Med Sci 14, 182–186.[CrossRef]
    [Google Scholar]
  23. Soltesz, V., Zeeberg, B. & Wadström, T. ( 1992;). Optimal survival of Helicobacter pylori under various transport conditions. J Clin Microbiol 30, 1453–1456.
    [Google Scholar]
  24. Sutton, I. & Neuberger, J. ( 2002;). Primary biliary cirrhosis: seeking the silent partner of autoimmunity. Gut 50, 743–746.[CrossRef]
    [Google Scholar]
  25. Verbaan, H., Hoffmann, G., Lindgren, S., Nilsson, S., Widell, A. & Eriksson, S. ( 1998;). Long-term outcome of chronic hepatitis C infection in a low-prevalence area. Scand J Gastroenterol 33, 650–655.[CrossRef]
    [Google Scholar]
  26. Wadström, T., Ljungh, Å. & Willén, R. ( 2001;). Primary biliary cirrhosis and primary sclerosing cholangitis are of infectious origin! Gut 49, 454. 454.
    [Google Scholar]
  27. Ward, J. M., Benveniste, R. E., Fox, C. H., Battles, J. K., Gonda, M. A. & Tully, J. G. ( 1996;). Autoimmunity in chronic active Helicobacter hepatitis of mice.Serum antibodies and expression of heat shock protein 70 in liver. Am J Pathol 148, 509–517.
    [Google Scholar]
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