1887

Abstract

Randomly amplified polymorphic DNA (RAPD) and automated amplified-fragment length polymorphism (AFLP) techniques with fluorescently labelled primers were used to type non-serotypable (NTHI) isolates. Eighty-seven isolates from healthy children attending day-care centres or living at orphanages in southern Poland were investigated. Through comparison of the AFLP data with RAPD analysis, it has been concluded that the discriminatory power of AFLP for NTHI typing is higher than RAPD. Generally, the NTHI isolates analysed were highly heterogeneous, as detected with a dIII/I AFLP genotyping scheme on intra/inter similarity levels of 94 and 96 % using Pearson's correlation coefficient. The range of similarity values found for isolates from children permanently residing at a particular day-care centre was much wider than that for isolates from orphanages. AFLP can efficiently access NTHI strain diversity and can monitor their turn-over for comparative typing in local and inter-local epidemiological investigations.

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2003-11-01
2019-11-18
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References

  1. Bolduc, G. R., Bouchet, V., Jiang, R.-Z., Geisselsoder, J., Truong-Bolduc, Q. C., Rice, P. A., Pelton, S. I. & Goldstein, R. ( 2000;). Variability of outer membrane protein P1 and its evaluation as a vaccine candidate against experimental otitis media due to nontypeable Haemophilus influenzae: an unambiguous, multifaceted approach. Infect Immun 68, 4505–4517.[CrossRef]
    [Google Scholar]
  2. Cerquetti, M., Ciofi degli Atti, M. L., Renna, G., Tozzi, A. E., Garlaschi, M. L. & Mastrantonio, P. ( 2000;). Characterization of non-type b Haemophilus influenzae strains isolated from patients with invasive disease.The HI Study Group. J Clin Microbiol 38, 4649–4652.
    [Google Scholar]
  3. Desai, M., Tanna, A., Wall, R., Efstratiou, A., George, R. & Stanley, J. ( 1998;). Fluorescent amplified-fragment length polymorphism analysis of an outbreak of group A streptococcal invasive disease. J Clin Microbiol 36, 3133–3137.
    [Google Scholar]
  4. Everitt, B. ( 1993;). Cluster Analysis, 3rd edn. London: Arnold.
  5. Faden, H., Duffy, L., Williams, A., Krystofik, D. A. & Wolf, J. ( 1995;). Epidemiology of nasopharyngeal colonization with nontypeable Haemophilus influenzae in the first 2 years of life. J Infect Dis 172, 132–135.[CrossRef]
    [Google Scholar]
  6. Falla, T. J., Dobson, S. R. M., Crook, D. W. M. & 7 other authors ( 1993;). Population-based study of non-typable Haemophilus influenzae invasive disease in children and neonates. Lancet 341, 851–854.[CrossRef]
    [Google Scholar]
  7. Falla, T. J., Crook, D. W., Brophy, L. N., Maskell, D., Kroll, J. S. & Moxon, E. R. ( 1994;). PCR for capsular typing of Haemophilus influenzae. J Clin Microbiol 32, 2382–2386.
    [Google Scholar]
  8. Fuste, M. C., Pineda, M. A., Palomar, J., Vinas, M. & Loren, J. G. ( 1996;). Clonality of multidrug-resistant nontypeable strains of Haemophilus influenzae. J Clin Microbiol 34, 2760–2765.
    [Google Scholar]
  9. Gazagne, L., Delmas, C., Bingen, E. & Dabernat, H. ( 1998;). Molecular epidemiology of ampicillin-resistant non-β-lactamase-producing Haemophilus influenzae. J Clin Microbiol 36, 3629–3635.
    [Google Scholar]
  10. Gilsdorf, J. R. ( 1998;). Antigenic diversity and gene polymorphisms in Haemophilus influenzae. Infect Immun 66, 5053–5059.
    [Google Scholar]
  11. Grady, R., Desai, M., O'Neill, G., Cookson, B. & Stanley, J. ( 1999;). Genotyping of epidemic methicillin-resistant Staphylococcus aureus phage type 15 isolates by fluorescent amplified-fragment length polymorphism analysis. J Clin Microbiol 37, 3198–3203.
    [Google Scholar]
  12. Hargreaves, R. M., Slack, M. P. E., Howard, A. J., Anderson, E. & Ramsay, M. E. ( 1996;). Changing patterns of invasive Haemophilus influenzae disease in England and Wales after introduction of the Hib vaccination programme. BMJ 312, 160–161.[CrossRef]
    [Google Scholar]
  13. Heersma, H. F., Kremer, K., van Soolingen, D. & Hauman, J. ( 2001;). Setting up intra- and inter-laboratory databases of electrophoretic profiles. In New Approaches for the Generation and Analysis of Microbial Typing Data, pp. 47–77. Edited by L. Dijkshoorn, K. J. Towner & M. Struelens. Amsterdam: Elsevier.
  14. Huys, G., Coopman, R., Janssen, P. & Kersters K. ( 1996;). High-resolution genotypic analysis of the genus Aeromonas by AFLP fingerprinting. Int J Syst Bacteriol 46, 572–580.[CrossRef]
    [Google Scholar]
  15. Janssen, P. J. D. ( 2001;). Selective restriction fragment amplification by AFLP. In New Approaches for the Generation and Analysis of Microbial Typing Data, pp. 177–210. Edited by L. Dijkshoorn, K. J. Towner & M. Struelens. Amsterdam: Elsevier.
  16. Jordens, J. Z., Leaves, N. I., Anderson, E. C. & Slack, M. P. E. ( 1993;). Polymerase chain reaction-based strain characterization of noncapsulate Haemophilus influenzae. J Clin Microbiol 31, 2981–2987.
    [Google Scholar]
  17. Kokotovic, B., Friis, N. F., Jensen, J. S. & Ahrens, P. ( 1999;). Amplified-fragment length polymorphism fingerprinting of Mycoplasma species. J Clin Microbiol 37, 3300–3307.
    [Google Scholar]
  18. Leaves, N. I. & Jordens, J. Z. ( 1996;). Analysis of the prevaccine population of noncapsulate Haemophilus influenzae and identification of a putative epidemic clone. Eur J Clin Microbiol Infect Dis 15, 410–414.[CrossRef]
    [Google Scholar]
  19. McLauchlin, J., Ripabelli, G., Brett, M. M. & Threlfall, E. J. ( 2000;). Amplified fragment length polymorphism (AFLP) analysis of Clostridium perfringens for epidemiological typing. Int J Food Microbiol 56, 21–28.[CrossRef]
    [Google Scholar]
  20. Meats, E., Feil, E. J., Stringer, S., Cody, A. J., Goldstein, R., Kroll, J. S., Popovic, T. & Spratt, B. G. ( 2003;). Characterization of encapsulated and noncapsulated Haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing. J Clin Microbiol 41, 1623–1636.[CrossRef]
    [Google Scholar]
  21. Moor, P. E., Collignon, P. C. & Gilbert, G. L. ( 1999;). Pulsed-field gel electrophoresis used to investigate genetic diversity of Haemophilus influenzae type b isolates in Australia shows differences between Aboriginal and non-Aboriginal isolates. J Clin Microbiol 37, 1524–1531.
    [Google Scholar]
  22. Mori, E., Lio, P., Daly, S., Damiani, G., Perito, B. & Fani, R. ( 1999;). Molecular nature of RAPD markers from Haemophilus influenzae Rd genome. Res Microbiol 150, 83–93.[CrossRef]
    [Google Scholar]
  23. Musser, J. M., Kroll, J. S., Moxon, E. R. & Selander, R. K. ( 1988;). Clonal population structure of encapsulated Haemophilus influenzae. Infect Immun 56, 1837–1845.
    [Google Scholar]
  24. Olive, D. M. & Bean, P. ( 1999;). Principles and applications of methods for DNA-based typing of microbial organisms. J Clin Microbiol 37, 1661–1669.
    [Google Scholar]
  25. Omikunle, A., Takahashi, S., Ogilvie, C. L., Wang, Y., Rodriguez, C. A., St Geme, J. W., III & Adderson, E. E. ( 2002;). Limited genetic diversity of recent invasive isolates of non-serotype b encapsulated Haemophilus influenzae. J Clin Microbiol 40, 1264–1270.[CrossRef]
    [Google Scholar]
  26. Peerbooms, P. G. H., Engelen, M. N., Stokman, D. A. J., van Benthem, B. H. B., van Weert, M.-L., Bruisten, S. M., van Belkum, A. & Coutinho, R. A. ( 2002;). Nasopharyngeal carriage of potential bacterial pathogens related to day care attendance, with special reference to the molecular epidemiology of Haemophilus influenzae. J Clin Microbiol 40, 2832–2836.[CrossRef]
    [Google Scholar]
  27. Peltola, H. ( 2000;). Worldwide Haemophilus influenzae type b disease at the beginning of the 21st century: global analysis of the disease burden 25 years after the use of the polysaccharide vaccine and a decade after the advent of conjugates. Clin Microbiol Rev 13, 302–317.[CrossRef]
    [Google Scholar]
  28. Pettigrew, M. M., Foxman, B., Ecevit, Z., Marrs, C. F. & Gilsdorf, J. ( 2002;). Use of pulsed-field gel electrophoresis, enterobacterial repetitive intergenic consensus typing, and automated ribotyping to assess genomic variability among strains of nontypeable Haemophilus influenzae. J Clin Microbiol 40, 660–662.[CrossRef]
    [Google Scholar]
  29. Saito, M., Umeda, A. & Yoshida, S. ( 1999;). Subtyping of Haemophilus influenzae strains by pulsed-field gel electrophoresis. J Clin Microbiol 37, 2142–2147.
    [Google Scholar]
  30. Savelkoul, P. H. M., Aarts, H. J. M., de Haas, J., Dijkshoorn, L., Duim, B., Otsen, M., Rademaker, J. L. W., Schouls, L. & Lenstra, J. A. ( 1999;). Amplified-fragment length polymorphism analysis: the state of an art. J Clin Microbiol 37, 3083–3091.
    [Google Scholar]
  31. Smith-Vaughan, H. C., Spiprakash, K. S., Leach, A. J., Mathews, J. D. & Kemp, D. J. ( 1998;). Low genetic diversity of Haemophilus influenzae type b compared to nonencapsulated H.influenzae in a population in which H. influenzae is highly endemic. Infect Immun 66, 3403–3409.
    [Google Scholar]
  32. Spinola, S. M., Peacock, J., Denny, F. W., Smith, D. L. & Cannon, J. G. ( 1986;). Epidemiology of colonization by nontypable Haemophilus influenzae in children: a longitudinal study. J Infect Dis 154, 100–109.[CrossRef]
    [Google Scholar]
  33. St Geme, J. W., III, Takala, A., Esko, E. & Falkow, S. ( 1994;). Evidence for capsule gene sequences among pharyngeal isolates of nontypeable Haemophilus influenzae. J Infect Dis 169, 337–342.[CrossRef]
    [Google Scholar]
  34. Trottier, S., Stenberg, K. & Svanborg-Eden, C. ( 1989;). Turnover of nontypable Haemophilus influenzae in the nasopharynges of healthy children. J Clin Microbiol 27, 2175–2179.
    [Google Scholar]
  35. Urwin, G., Krohn, J. A., Deaver-Robinson, K., Wenger, J. D. & Farley, M. M. ( 1996;). Invasive disease due to Haemophilus influenzae serotype f: clinical and epidemiologic characteristics in the H.influenzae serotype b vaccine era. The Haemophilus influenzae Study Group. Clin Infect Dis 22, 1069–1076.[CrossRef]
    [Google Scholar]
  36. van Alphen, L., Eijk, P., Geelen-van den Broek, L. & Dankert, J. ( 1991;). Immunochemical characterization of variable epitopes of outer membrane protein P2 of nontypeable Haemophilus influenzae. Infect Immun 59, 247–252.
    [Google Scholar]
  37. van Alphen, L., Caugant, D. A., Duim, B., O'Rourke, M. & Bowler, L. D. ( 1997;). Differences in genetic diversity of nonencapsulated Haemophilus influenzae from various diseases. Microbiology 143, 1423–1431.[CrossRef]
    [Google Scholar]
  38. van Belkum, A., Duim, B., Regelink, A., Moller, L., Quint, W. & van Alphen, L. ( 1994;). Genomic DNA fingerprinting of clinical Haemophilus influenzae isolates by polymerase chain reaction amplification: comparison with major outer-membrane protein and restriction fragment length polymorphism analysis. J Med Microbiol 41, 63–68.[CrossRef]
    [Google Scholar]
  39. van Belkum, A., Scherer, S., van Leeuwen, W., Willemse, D., van Alphen, L. & Verbrugh, H. ( 1997;). Variable number of tandem repeats in clinical strains of Haemophilus influenzae. Infect Immun 65, 5017–5027.
    [Google Scholar]
  40. van Eldere, J., Janssen, P., Hoefnagels-Schuermans, A., van Lierde, S. & Peetermans, W. E. ( 1999;). Amplified-fragment length polymorphism analysis versus macro-restriction fragment analysis for molecular typing of Streptococcus pneumoniae isolates. J Clin Microbiol 37, 2053–2057.
    [Google Scholar]
  41. Vos, P. & Kuiper, M. ( 1998;). AFLP analysis. In DNA Markers, Protocols, Applications, and Overviews, pp. 115–131. Edited by G. Caetano-Anolles & P. M. Gresshoff. Chichester: Wiley.
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