1887

Abstract

A comprehensive study was performed to examine incidence, species distribution, drugs sensitivity, virulence genes and molecular fingerprints of species isolated from patients with acute diarrhoea over a period of 2 years in Kolkata, India. Following the Aerokey II scheme, more than 95 % of strains were identified to species level. Seven different species were encountered in this study, with being dominant, followed by and biovar sobria. Thirty different serotypes were encountered, with O16, O83 and O85 being dominant, but no serotype was associated specifically with a single species. The majority of strains exhibited multidrug resistance. The and genes, which encode heat-labile cytotonic and cytotoxic enterotoxins, were respectively found in 71.9 and 20.1 % of strains examined. Only 2.4 % of strains carried the heat-stable cytotonic enterotoxin () gene. The gene was found in 28 % of strains. With few exceptions, genomic diversity of strains belonging to the same serotype was observed by random amplification of polymorphic DNA PCR and ribotyping. Different species of and different clones of species seem to be associated with hospitalized cases of diarrhoea in Kolkata, India.

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2004-06-01
2019-11-18
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