1887

Abstract

The occurrence of multidrug-resistant or meticillin-resistant (MRSA) has become an important issue in clinics. This study evaluated a combinatorial treatment approach by using the well-documented antibacterial protein apo-bovine lactoferrin (apo-bLf) or its hydrolysate and specific probiotic supernatants for controlling MRSA infection. Clinical MRSA strains were isolated from different patient specimens. Apo-bLf-hydrolysate possessed stronger anti-MRSA activity than complete bLf in that it inhibited the growth of most MRSA strains tested . Otherwise, the supernatants produced by (ATCC 11739), subsp. (ATCC 15707) and subsp. (BCRC 17394) inhibited the growth of various MRSA strains. Further, or subsp. supernatant plus apo-bLf or bLf-hydrolysate led to partially synergistic to synergistic growth-inhibitory activity against MRSA strains. However, and not subsp. or subsp. was observed to resist the antibacterial activity of both apo-Lf and bLf-hydrolysate. Therefore, it is suggested that could be the best candidate to be used with apo-bLf or bLf-hydrolysate as a live supplement against MRSA infections.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.052639-0
2013-12-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/62/12/1845.html?itemId=/content/journal/jmm/10.1099/jmm.0.052639-0&mimeType=html&fmt=ahah

References

  1. Agostoni C., Axelsson I., Goulet O., Koletzko B., Michaelsen K. F., Puntis J. W., Rigo J., Shamir R., Szajewska H., Turck D..ESPGHAN Committee on Nutrition ( 2004;). Prebiotic oligosaccharides in dietetic products for infants: a commentary by the ESPGHAN Committee on Nutrition. . J Pediatr Gastroenterol Nutr 39:, 465–473. [CrossRef][PubMed]
    [Google Scholar]
  2. Aguila A., Herrera A. G., Morrison D., Cosgrove B., Perojo A., Montesinos I., Pérez J., Sierra G., Gemmell C. G., Brock J. H.. ( 2001;). Bacteriostatic activity of human lactoferrin against Staphylococcus aureus is a function of its iron-binding properties and is not influenced by antibiotic resistance. . FEMS Immunol Med Microbiol 31:, 145–152. [CrossRef][PubMed]
    [Google Scholar]
  3. Arqués J. L., Fernández J., Gaya P., Nuñez M., Rodríguez E., Medina M.. ( 2004;). Antimicrobial activity of reuterin in combination with nisin against food-borne pathogens. . Int J Food Microbiol 95:, 225–229. [CrossRef][PubMed]
    [Google Scholar]
  4. Baveye S., Elass E., Mazurier J., Spik G., Legrand D.. ( 1999;). Lactoferrin: a multifunctional glycoprotein involved in the modulation of the inflammatory process. . Clin Chem Lab Med 37:, 281–286. [CrossRef][PubMed]
    [Google Scholar]
  5. Bellamy W., Takase M., Wakabayashi H., Kawase K., Tomita M.. ( 1992;). Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. . J Appl Bacteriol 73:, 472–479. [CrossRef][PubMed]
    [Google Scholar]
  6. Bellamy W. R., Wakabayashi H., Takase M., Kawase K., Shimamura S., Tomita M.. ( 1993;). Role of cell-binding in the antibacterial mechanism of lactoferricin B. . J Appl Bacteriol 75:, 478–484.[PubMed]
    [Google Scholar]
  7. Bergamaschi A., Magrini A., Pietroiusti A.. ( 2007;). Recent advances in the treatment of Helicobacter pylori infection. . Recent Pat Antiinfect Drug Discov 2:, 197–205. [CrossRef][PubMed]
    [Google Scholar]
  8. Brock J. H.. ( 1980;). Lactoferrin in human milk: its role in iron absorption and protection against enteric infection in the newborn infant. . Arch Dis Child 55:, 417–421. [CrossRef][PubMed]
    [Google Scholar]
  9. Bullen J. J.. ( 1975;). Iron-binding proteins in milk and resistance to Escherichia coli infection in infants. . Postgrad Med J 51: (Suppl 3), 67–70.[PubMed]
    [Google Scholar]
  10. Casey J. A., Cosgrove S. E., Stewart W. F., Pollak J., Schwartz B. S.. ( 2013;). A population-based study of the epidemiology and clinical features of methicillin-resistant Staphylococcus aureus infection in Pennsylvania, 2001-2010. . Epidemiol Infect 141:, 1166–1179. [CrossRef][PubMed]
    [Google Scholar]
  11. Chen P. W., Ho S. P., Shyu C. L., Mao F. C.. ( 2004;). Effects of bovine lactoferrin hydrolysate on the in vitro antimicrobial susceptibility of Escherichia coli strains isolated from baby pigs. . Am J Vet Res 65:, 131–137. [CrossRef][PubMed]
    [Google Scholar]
  12. Chierici R.. ( 2001;). Antimicrobial actions of lactoferrin. . Adv Nutr Res 10:, 247–269.[PubMed]
    [Google Scholar]
  13. de Bortoli N., Leonardi G., Ciancia E., Merlo A., Bellini M., Costa F., Mumolo M. G., Ricchiuti A., Cristiani F.. & other authors ( 2007;). Helicobacter pylori eradication: a randomized prospective study of triple therapy versus triple therapy plus lactoferrin and probiotics. . Am J Gastroenterol 102:, 951–956. [CrossRef][PubMed]
    [Google Scholar]
  14. Diarra M. S., Petitclerc D., Lacasse P.. ( 2002;). Effect of lactoferrin in combination with penicillin on the morphology and the physiology of Staphylococcus aureus isolated from bovine mastitis. . J Dairy Sci 85:, 1141–1149. [CrossRef][PubMed]
    [Google Scholar]
  15. Elass-Rochard E., Roseanu A., Legrand D., Trif M., Salmon V., Motas C., Montreuil J., Spik G.. ( 1995;). Lactoferrin-lipopolysaccharide interaction: involvement of the 28-34 loop region of human lactoferrin in the high-affinity binding to Escherichia coli 055B5 lipopolysaccharide. . Biochem J 312:, 839–845.[PubMed]
    [Google Scholar]
  16. Ellison R. T. III, Giehl T. J.. ( 1991;). Killing of gram-negative bacteria by lactoferrin and lysozyme. . J Clin Invest 88:, 1080–1091. [CrossRef][PubMed]
    [Google Scholar]
  17. Ellison R. T. III, Giehl T. J., LaForce F. M.. ( 1988;). Damage of the outer membrane of enteric gram-negative bacteria by lactoferrin and transferrin. . Infect Immun 56:, 2774–2781.[PubMed]
    [Google Scholar]
  18. Farnaud S., Evans R. W.. ( 2003;). Lactoferrin–a multifunctional protein with antimicrobial properties. . Mol Immunol 40:, 395–405. [CrossRef][PubMed]
    [Google Scholar]
  19. Gaggìa F., Mattarelli P., Biavati B.. ( 2010;). Probiotics and prebiotics in animal feeding for safe food production. . Int J Food Microbiol 141: (Suppl 1), S15–S28. [CrossRef][PubMed]
    [Google Scholar]
  20. Gould I. M., David M. Z., Esposito S., Garau J., Lina G., Mazzei T., Peters G.. ( 2012;). New insights into meticillin-resistant Staphylococcus aureus (MRSA) pathogenesis, treatment and resistance. . Int J Antimicrob Agents 39:, 96–104. [CrossRef][PubMed]
    [Google Scholar]
  21. Kim W. S., Ohashi M., Tanaka T., Kumura H., Kim G. Y., Kwon I. K., Goh J. S., Shimazaki K.. ( 2004;). Growth-promoting effects of lactoferrin on L. acidophilus and Bifidobacterium spp. . Biometals 17:, 279–283. [CrossRef][PubMed]
    [Google Scholar]
  22. Legrand D., Mazurier J.. ( 2010;). A critical review of the roles of host lactoferrin in immunity. . Biometals 23:, 365–376. [CrossRef][PubMed]
    [Google Scholar]
  23. Levay P. F., Viljoen M.. ( 1995;). Lactoferrin: a general review. . Haematologica 80:, 252–267.[PubMed]
    [Google Scholar]
  24. Masco L., Ventura M., Zink R., Huys G., Swings J.. ( 2004;). Polyphasic taxonomic analysis of Bifidobacterium animalis and Bifidobacterium lactis reveals relatedness at the subspecies level: reclassification of Bifidobacterium animalis as Bifidobacterium animalis subsp. animalis subsp. nov. and Bifidobacterium lactis as Bifidobacterium animalis subsp. lactis subsp. nov. . Int J Syst Evol Microbiol 54:, 1137–1143. [CrossRef][PubMed]
    [Google Scholar]
  25. McDougal L. K., Thornsberry C.. ( 1984;). New recommendations for disk diffusion antimicrobial susceptibility tests for methicillin-resistant (heteroresistant) staphylococci. . J Clin Microbiol 19:, 482–488.[PubMed]
    [Google Scholar]
  26. Meile L., Ludwig W., Rueger U., Gut C., Kaufmann P., Dasen G., Wenger S., Teuber T.. ( 1997;). Bifidobacterium lactis sp. nov., a moderately oxygen tolerant species isolated from fermented milk. . Syst Appl Microbiol 20:, 57–64. [CrossRef]
    [Google Scholar]
  27. Olivares M., Díaz-Ropero M. P., Martín R., Rodríguez J. M., Xaus J.. ( 2006;). Antimicrobial potential of four Lactobacillus strains isolated from breast milk. . J Appl Microbiol 101:, 72–79. [CrossRef][PubMed]
    [Google Scholar]
  28. Onanuga A., Awhowho G. O.. ( 2012;). Antimicrobial resistance of Staphylococcus aureus strains from patients with urinary tract infections in Yenagoa, Nigeria. . J Pharm Bioallied Sci 4:, 226–230. [CrossRef][PubMed]
    [Google Scholar]
  29. Orsi N.. ( 2004;). The antimicrobial activity of lactoferrin: current status and perspectives. . Biometals 17:, 189–196. [CrossRef][PubMed]
    [Google Scholar]
  30. Petschow B. W., Talbott R. D., Batema R. P.. ( 1999;). Ability of lactoferrin to promote the growth of Bifidobacterium spp. in vitro is independent of receptor binding capacity and iron saturation level. . J Med Microbiol 48:, 541–549. [CrossRef][PubMed]
    [Google Scholar]
  31. Resta O., Foschino-Barbaro M. P., Bonfitto P., Talamo S., Legari G., De Pergola G., Minenna A., Giorgino R.. ( 2000;). Prevalence and mechanisms of diurnal hypercapnia in a sample of morbidly obese subjects with obstructive sleep apnoea. . Respir Med 94:, 240–246. [CrossRef][PubMed]
    [Google Scholar]
  32. Schrezenmeir J., de Vrese M.. ( 2001;). Probiotics, prebiotics, and synbiotics – approaching a definition. . Am J Clin Nutr 73: (Suppl), 361S–364S.[PubMed]
    [Google Scholar]
  33. Singh P. K., Tack B. F., McCray P. B. Jr, Welsh M. J.. ( 2000;). Synergistic and additive killing by antimicrobial factors found in human airway surface liquid. . Am J Physiol Lung Cell Mol Physiol 279:, L799–L805.[PubMed]
    [Google Scholar]
  34. Sopirala M. M., Mangino J. E., Gebreyes W. A., Biller B., Bannerman T., Balada-Llasat J. M., Pancholi P.. ( 2010;). Synergy testing by Etest, microdilution checkerboard, and time-kill methods for pan-drug-resistant Acinetobacter baumannii. . Antimicrob Agents Chemother 54:, 4678–4683. [CrossRef][PubMed]
    [Google Scholar]
  35. Talarico T. L., Casas I. A., Chung T. C., Dobrogosz W. J.. ( 1988;). Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri. . Antimicrob Agents Chemother 32:, 1854–1858. [CrossRef][PubMed]
    [Google Scholar]
  36. Teitelbaum J. E., Walker W. A.. ( 2002;). Nutritional impact of pre- and probiotics as protective gastrointestinal organisms. . Annu Rev Nutr 22:, 107–138. [CrossRef][PubMed]
    [Google Scholar]
  37. Tian H., Maddox I. S., Ferguson L. R., Shu Q.. ( 2010;). Influence of bovine lactoferrin on selected probiotic bacteria and intestinal pathogens. . Biometals 23:, 593–596. [CrossRef][PubMed]
    [Google Scholar]
  38. Tomita M., Bellamy W., Takase M., Yamauchi K., Wakabayashi H., Kawase K.. ( 1991;). Potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin. . J Dairy Sci 74:, 4137–4142. [CrossRef][PubMed]
    [Google Scholar]
  39. Tomita M., Wakabayashi H., Shin K., Yamauchi K., Yaeshima T., Iwatsuki K.. ( 2009;). Twenty-five years of research on bovine lactoferrin applications. . Biochimie 91:, 52–57. [CrossRef][PubMed]
    [Google Scholar]
  40. Valenti P., Antonini G.. ( 2005;). Lactoferrin. . Cell Mol Life Sci 62:, 2576–2587. [CrossRef][PubMed]
    [Google Scholar]
  41. van der Linden D. S., Short D., Dittmann A., Yu P. L.. ( 2009;). Synergistic effects of ovine-derived cathelicidins and other antimicrobials against Escherichia coli O157:H7 and Staphylococcus aureus 1056 MRSA. . Biotechnol Lett 31:, 1265–1267. [CrossRef][PubMed]
    [Google Scholar]
  42. Wang J., Tian Z.. ( 2010;). Recent advances in lactoferrin research and development during the past two years (2007-2009): in lieu of a preface of the Special Issue Lactoferrin. . Biometals 23:, 355–357. [CrossRef][PubMed]
    [Google Scholar]
  43. Yamauchi K., Wakabayashi H., Shin K., Takase M.. ( 2006;). Bovine lactoferrin: benefits and mechanism of action against infections. . Biochem Cell Biol 84:, 291–296. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.052639-0
Loading
/content/journal/jmm/10.1099/jmm.0.052639-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error