1887

Abstract

Elastolytic and elastase inhibitory activities were investigated for 13 strains of , three strains of and three strains of . Nine of the 13 strains of and all strains of demonstrated elastase activity (more than 1 unit ml). Six of the 13 strains of and all strains of expressed elastase inhibitory activity (more than 2 units ml). However, no elastase or elastase inhibitory activities were observed with . It was also found that crude elastase inhibitors from six strains of and two strains of were stable to heat treatment at 100 °C for 10 min. In addition, human leukocyte elastases were inhibited by crude elastase inhibitors from and ; however, no effect was observed on the elastase derived from porcine pancreas.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05248-0
2004-05-01
2020-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/5/JM530501.html?itemId=/content/journal/jmm/10.1099/jmm.0.05248-0&mimeType=html&fmt=ahah

References

  1. Blanco J. L., Hontecillas R., Bouza E., Blanco I., Pelaez T., Munoz P., Perez Molina J., Garcia M. E. 2002; Correlation between the elastase activity index and invasiveness of clinical isolates of Aspergillus fumigatus . J Clin Microbiol 40:1811–1813 [CrossRef]
    [Google Scholar]
  2. Denning D. W., Ward P. N., Fenelon L. E., Benbow E. W. 1992; Lack of vessel wall elastolysis in human invasive pulmonary aspergillosis. Infect Immun 60:5153–5156
    [Google Scholar]
  3. Frosco M. B., Chase T. Jr, Macmillan J. D. 1992; Purification and properties of elastase from Aspergillus fumigatus . Infect Immun 60:728–734
    [Google Scholar]
  4. Frosco M. B., Chase T. Jr, Macmillan J. D. 1994; The effect of elastase-specific monoclonal and polyclonal antibodies on the virulence of Aspergillus fumigatus in immunocompromised mice. Mycopathologia 125:65–76 [CrossRef]
    [Google Scholar]
  5. Hasegawa Y., Nikai T., Yoshikawa Y., Sugihara H., Ogawa K., Takagi K. 1994; Elastase activity from Aspergillus species and inhibition by urinastatin. Jpn J Med Mycol 35:293–298 [CrossRef]
    [Google Scholar]
  6. Hasegawa Y., Nikai T., Yamashita R., Yoshikawa Y., Sugihara H., Ogawa K., Mizuno M. 1995; Isolation and characterization of elastolytic proteinase from Aspergillus fumigatus . Jpn J Med Mycol 36:235–243 [CrossRef]
    [Google Scholar]
  7. Hasegawa Y., Nikai T., Okumura Y., Ogawa K., Sugihara H. 1997; Characterization of elastolytic proteinase from Aspergillus flavus : comparison of the elastolytic proteinase from Aspergillus fumigatus . Jpn J Med Mycol 38:53–59 [CrossRef]
    [Google Scholar]
  8. Iadarola P., Lungarella G., Martorana P. A. & 8 other authors; 1998; Lung injury and degradation of extracellular matrix components by Aspergillus fumigatus serine proteinase. Exp Lung Res 24:233–251 [CrossRef]
    [Google Scholar]
  9. Kawabata K., Suzuki M., Sugitani M., Imaki K., Toda M., Miyamoto T. 1991; ONO-5046, a novel inhibitor of human neutrophil elastase. Biochem Biophys Res Commun 177:814–820 [CrossRef]
    [Google Scholar]
  10. Kolattukudy P. E., Lee J. D., Rogers L. M., Zimmerman P., Ceselski S., Fox B., Stein B., Copelan E. A. 1993; Evidence for possible involvement of an elastolytic serine protease in aspergillosis. Infect Immun 61:2357–2368
    [Google Scholar]
  11. Maesaki S., Kohno S., Tanaka K. & 7 other authors; 1993; A case of pulmonary aspergilloma successfully treated with combination therapy of intracavitary injection of amphotericin B and intravenous administration of urinastatin. Nihon Kyobu Shikkan Gakkai Zasshi 31:1327–1331 (in Japanese
    [Google Scholar]
  12. Mellon J. E., Cotty P. J. 1995; Expression of elastinolytic activity among isolates in Aspergillus section flavi. Mycopathologia 131:115–120 [CrossRef]
    [Google Scholar]
  13. Monod M., Togni G., Rahalison L., Frenk E. 1991; Isolation and characterization of an extracellular alkaline protease of Aspergillus fumigatus . J Med Microbiol 35:23–28 [CrossRef]
    [Google Scholar]
  14. Ogawa K. 1997; Treatment of chronic pulmonary aspergillosis (fungus ball type, mural thickness type). Kekkaku 72:119–124 (in Japanese
    [Google Scholar]
  15. Ogawa K., Taki F., Takagi K., Furui H., Tsuda M., Honda K., Sasaki T., Sasamoto M., Miwa T. 1992; Study of elastase production by Aspergillus species and inhibition of their elastase activity by drugs. Kokyuu 11:880–886 (in Japanese
    [Google Scholar]
  16. Ohno H., Yoshida M., Takahashi Y., Omura S. 1980; Improvement of the productivity of elasnin, a specific elastase inhibitor, by Streptomyces noboritoensis KM-2753. J Antibiot (Tokyo 33:474–479 [CrossRef]
    [Google Scholar]
  17. Reichard U., Buttner S., Eiffert H., Staib F., Ruchel R. 1990; Purification and characterization of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue. J Med Microbiol 33:243–251 [CrossRef]
    [Google Scholar]
  18. Rhodes J. C., Amlung T. W., Miller M. S. 1990; Isolation and characterization of an elastinolytic proteinase from Aspergillus flavus . Infect Immun 58:2529–2534
    [Google Scholar]
  19. Sugimura S., Yoshida K., Oba H., Hashiguchi K., Nakajima M., Moriya O., Okimoto N., Niki Y., Soejima R. 1994; Two cases of invasive pulmonary aspergillosis in non-immunocompromised hosts. Nihon Kyobu Shikkan Gakkai Zasshi 32:1032–1037 (in Japanese
    [Google Scholar]
  20. Umezawa H., Aoyagi T., Okura A., Morishima H., Takeuchi T., Okami Y. 1973; Letter: Elastatinal, a new elastase inhibitor produced by actinomycetes. J Antibiot (Tokyo) 26:787–789 [CrossRef]
    [Google Scholar]
  21. Wiedow O., Schroder J. M., Gregory H., Young J. A., Christophers E. 1990; Elafin: an elastase-specific inhibitor of human skin.Purification, characterization, and complete amino acid sequence. J Biol Chem 265:14791–14795
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05248-0
Loading
/content/journal/jmm/10.1099/jmm.0.05248-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error