1887

Abstract

Outbreaks of leptospirosis that present with predominant pulmonary signs and symptoms have been occurring in the Andaman Islands since the late 1980s. Before this, pulmonary haemorrhage had not been observed as a common complication of leptospirosis in India. During an outbreak on North Andaman in 1997, four leptospire isolates were obtained from blood of a fatal case and three other patients who recovered. These isolates were characterized using serological and molecular techniques. Cross-agglutination absorption tests and microscopic agglutination tests using mAbs were used for serological characterization. Genetic typing was done using DNA sequencing of PCR products. Serologically, the isolates were closely related to strain Valbuzzi serovar Valbuzzi of serogroup Grippotyphosa. The sequences of PCR products from these isolates were compared with those of 45 strains belonging to seven species. The isolates showed 97.5–100 % sequence similarity to reference strains belonging to , indicating that the isolates belong to . Serogroups Icterohaemorrhagiae and Australis have been incriminated as the cause of pulmonary haemorrhage in China, Korea and Australia. The four isolates characterized in the present study were obtained from patients with similar symptoms. However, they belonged to serovar Valbuzzi of serogroup Grippotyphosa, indicating that serogroups other than Icterohaemorrhagiae and Australis can also cause pulmonary haemorrhage.

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2003-10-01
2019-10-17
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References

  1. Brenner, D. J., Kaufmann, A. F., Sulzer, K. R., Steigerwalt, A. G., Rogers, F. C. & Weyant, R. S. ( 1999;). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp.nov. and four new Leptospira genomospecies. Int J Syst Bacteriol 49, 839–858.[CrossRef]
    [Google Scholar]
  2. Desmecht, M., Korver, H. & Terpstra, W. J. ( 1991;). Isolation of Leptospira interrogans serovar saxkoebing, grippotyphosa and copenhageni from muskrats in Belgium. Klaams Diergeneeskd Tijdschr 60, 59–63 (in Flemish).
    [Google Scholar]
  3. Dikken, H. & Kmety, E. ( 1978;). Serological typing methods of leptospires. Methods Microbiol 11, 259–307.
    [Google Scholar]
  4. Faine, S., Alder, B., Bolin, C. & Perolat, P. ( 1999;). Leptospira and Leptospirosis, 2nd edn. Melbourne: MedSci.
  5. Gravekamp, C., Van de Kemp, H., Franzen, M., Carrington, D., Schoone, G. J., Van Eys, G. J. J. M., Everard, C. O. R., Hartskeerl, R. A. & Terpstra, W. J. ( 1993;). Detection of seven species of pathogenic leptospires by PCR using two sets of primers. J Gen Microbiol 139, 1691–1700.[CrossRef]
    [Google Scholar]
  6. Johnson, R. C. & Harris, V. G. ( 1967;). Differentiation of pathogenic and saprophytic leptospires.I. Growth at low temperature. J Bacteriol 94, 27–31.
    [Google Scholar]
  7. Johnson, R. C. & Rogers, P. ( 1964;). Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J Bacteriol 88, 1618–1623.
    [Google Scholar]
  8. Korver, H., Kolk, A. H. J., Vingerhoed, J., Van Leeuwen, J. & Trepestra, W. J. ( 1988;). Classification of serovars of the Icterohaemorrhagiae serogroup by monoclonal antibodies. Isr J Vet Med 44, 15–18.
    [Google Scholar]
  9. Oh, H.-B., Chang, W.-H., Cho, M.-K., Seong, W.-K. & Park, K.-S. ( 1991;). Identification of new serovars yeonchon and hongchon belonging to Leptospira interrogans of the Icterohaemorrhagiae serogroup. J Korean Soc Microbiol 26, 253–262.
    [Google Scholar]
  10. Sehgal, S. C., Murhekar, M. V. & Sugunan, A. P. ( 1995;). Outbreak of leptospirosis with pulmonary involvement in North Andaman. Indian J Med Res 102, 9–12.
    [Google Scholar]
  11. Sehgal, S. C., Vijayachari, P., Smythe, L. D. & 7 other authors ( 2000;). Lai-like leptospira from the Andaman Islands. Indian J Med Res 112, 135–139.
    [Google Scholar]
  12. Sehgal, S. C., Sugunan, A. P. & Vijayachari, P. ( 2002;). Outbreak of leptospirosis after the cyclone in Orissa. Natl Med J India 15, 22–23.
    [Google Scholar]
  13. Simpson, F. G., Green, K. A., Haug, G. J. & Brookes, D. L. ( 1998;). Leptospirosis associated with severe pulmonary haemorrhage in Far North Queensland. Med J Aust 169, 151–153.
    [Google Scholar]
  14. Singh, S. S., Vijayachari, P., Sinha, A., Sugunan, A.P., Rasheed, M. A. & Sehgal, S. C. ( 1999;). Clinico-epidemiological study of hospitalized cases of severe leptospirosis. Indian J Med Res 109, 94–99.
    [Google Scholar]
  15. Taylor, R. J. & Goyle, A. N. ( 1931;). Leptospirosis in the Andamans. Supplement to Indian Journal of Medical Research, Memoir no. 20. Indian Research Fund Association. Calcutta: Thacker, Spink & Co.
  16. Terpstra, W. J. ( 1992;). Typing leptospira from the perspective of a reference laboratory. Acta Leiden 60, 79–87.
    [Google Scholar]
  17. Trevejo, R. T., Rigau-Perez, J. G., Ashford, D. A. & 14 other authors ( 1998;). Epidemic leptospirosis associated with pulmonary hemorrhage – Nicaragua, 1995. J Infect Dis 178, 1457–1463.[CrossRef]
    [Google Scholar]
  18. WHO ( 1999;). Leptospirosis, Australia, January 1998–March 1999.WHO/FAO Collaborating Centre for Reference and Research on Leptospirosis. Wkly Epidemiol Rec 74, 113–118.
    [Google Scholar]
  19. Wolff, J. W. ( 1954;). In The Laboratory Diagnosis of Leptospirosis, pp. 39–62. Springfield, IL: Charles C. Thomas.
  20. Zuerner, R. L. & Bolin, C. A. ( 1997;). Differentiation of Leptospira interrogans isolates by IS1500 hybridization and PCR assays. J Clin Microbiol 35, 2612–2617.
    [Google Scholar]
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