1887

Abstract

Chronic maxillary sinusitis is a chronic inflammatory condition in which the role of microbial infection remains undefined. Bacteria have been isolated from chronically inflamed sinuses; however, their role in the chronicity of inflammation is unknown. The objective of this study was to determine whether bacteria are present in clinical samples from chronic maxillary sinusitis and to assess the diversity of the flora present. Washes and/or tissue samples from endoscopic sinus surgery on 11 patients with chronic maxillary sinusitis were subjected to PCR amplification of bacterial 16S rDNA using three universal primer pairs, followed by cloning and sequencing. The samples were also assessed for the presence of bacteria and fungi by conventional culture methods. Viable bacteria and/or bacterial 16S rDNA were detected from maxillary sinus samples of five of the 11 patients examined (45 %). Three sinus samples were positive by both PCR and culture methods, one was positive only by PCR, and one only by culture. Thirteen bacterial species were identified: , , sp., , sp., sp., , , , , , and sp. Fungi were not detected. In one patient , and in another patient , were detected from both the sinus and the oral cavity using species-specific PCR primers. These results suggest that both aerobic and anaerobic bacteria can be detected in nearly half of chronic maxillary sinusitis cases.

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2003-07-01
2019-11-17
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References

  1. Ahmet, Z., Stanier, P., Harvey, D. & Holt, D. ( 1999;). New PCR primers for the sensitive detection and specific identification of group B β-hemolytic streptococci in cerebrospinal fluid. Mol Cell Probes 13, 349–357.[CrossRef]
    [Google Scholar]
  2. Aul, J. J., Anderson, K. W., Wadowsky, R. M., Doyle, W. J., Kingsley, L. A., Post, J. C. & Ehrlich, G. D. ( 1998;). Comparative evaluation of culture and PCR for the detection and determination of persistence of bacterial strains and DNAs in the Chinchilla laniger model of otitis media. Ann Otol Rhinol Laryngol 107, 508–513.[CrossRef]
    [Google Scholar]
  3. Beck, J. D., Pankow, J., Tyroler, H. A. & Offenbacher, S. ( 1999;). Dental infections and atherosclerosis. Am Heart J 138, S528–S533.[CrossRef]
    [Google Scholar]
  4. Biel, M. A., Brown, C. A., Levinson, R. M., Garvis, G. E., Paisner, H. M., Sigel, M. E. & Tedford, T. M. ( 1998;). Evaluation of the microbiology of chronic maxillary sinusitis. Ann Otol Rhinol Laryngol 107, 942–945.[CrossRef]
    [Google Scholar]
  5. Brook, I. & Yocum, P. ( 1999;). Immune response to Fusobacterium nucleatum and Prevotella intermedia in patients with chronic maxillary sinusitis. Ann Otol Rhinol Laryngol 108, 293–295.[CrossRef]
    [Google Scholar]
  6. Brook, I., Frazier, E. H. & Foote, P. A. ( 1997;). Microbiology of chronic maxillary sinusitis: comparison between specimens obtained by sinus endoscopy and by surgical drainage. J Med Microbiol 46, 430–432.[CrossRef]
    [Google Scholar]
  7. Brook, I., Yocum, P. & Shah, K. ( 2000;). Aerobic and anaerobic bacteriology of concurrent chronic otitis media with effusion and chronic sinusitis in children. Arch Otolaryngol Head Neck Surg 126, 174–176.[CrossRef]
    [Google Scholar]
  8. Brown, A. E., Rogers, J. D., Haase, E. M., Zelasko, P. M. & Scannapieco, F. A. ( 1999;). Prevalence of the amylase-binding protein A gene (abpA) in oral streptococci. J Clin Microbiol 37, 4081–4085.
    [Google Scholar]
  9. Chan, J. & Hadley, J. ( 2001;). The microbiology of chronic rhinosinusitis: results of a community surveillance study. Ear Nose Throat J 80, 143–145.
    [Google Scholar]
  10. Dymock, D., Weightman, A. J., Scully, C. & Wade, W. G. ( 1996;). Molecular analysis of microflora associated with dentoalveolar abscesses. J Clin Microbiol 34, 537–542.
    [Google Scholar]
  11. Finegold, S. M., Flynn, M. J., Rose, F. V., Jousimies-Somer, H., Jakielaszek, C., McTeague, M., Wexler, H. M., Berkowitz, E. & Wynne, B. ( 2002;). Bacteriologic findings associated with chronic bacterial maxillary sinusitis in adults. Clin Infect Dis 35, 428–433.[CrossRef]
    [Google Scholar]
  12. Garnier, F., Gerbaud, G., Courvalin, P. & Galimand, M. ( 1997;). Identification of clinically relevant viridans group streptococci to the species level by PCR. J Clin Microbiol 35, 2337–2341.
    [Google Scholar]
  13. Gram, T., Ahrens, P. & Nielsen, J. P. ( 1996;). Evaluation of a PCR for detection of Actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. Vet Microbiol 51, 95–104.[CrossRef]
    [Google Scholar]
  14. Grossi, S. G. & Genco, R. J. ( 1998;). Periodontal disease and diabetes mellitus: a two-way relationship. Ann Periodontol 3, 51–61.[CrossRef]
    [Google Scholar]
  15. Gwaltney, J. M., Jr ( 1996;). Acute community-acquired sinusitis. Clin Infect Dis 23, 1209–1223.[CrossRef]
    [Google Scholar]
  16. Hugenholtz, P., Pitulle, C., Hershberger, K. L. & Pace, N. R. ( 1998;). Novel division level bacterial diversity in a Yellowstone hot spring. J Bacteriol 180, 366–376.
    [Google Scholar]
  17. Hwang, P. H., Montone, K. T., Gannon, F. H., Senior, B. A., Lanza, D. C. & Kennedy, D. W. ( 1999;). Applications of in situ hybridization techniques in the diagnosis of chronic sinusitis. Am J Rhinol 13, 335–338.[CrossRef]
    [Google Scholar]
  18. Jaffe, R. I., Lane, J. D., Albury, S. V. & Niemeyer, D. M. ( 2000;). Rapid extraction from and direct identification in clinical samples of methicillin-resistant staphylococci using the PCR. J Clin Microbiol 38, 3407–3412.
    [Google Scholar]
  19. Jiang, R. S., Hsu, C. Y. & Jang, J. W. ( 1998;). Bacteriology of the maxillary and ethmoid sinuses in chronic sinusitis. J Laryngol Otol 112, 845–848.
    [Google Scholar]
  20. Jiang, R. S., Liang, K. L., Jang, J. W. & Hsu, C. Y. ( 1999;). Bacteriology of endoscopically normal maxillary sinuses. J Laryngol Otol 113, 825–828.
    [Google Scholar]
  21. Kato, C., Li, L., Tamaoka, J. & Horikoshi, K. ( 1997;). Molecular analyses of the sediment of the 11,000-m deep Mariana Trench. Extremophiles 1, 117–123.[CrossRef]
    [Google Scholar]
  22. Keech, D. R., Ramadan, H. & Mathers, P. ( 2000;). Analysis of aerobic bacterial strains found in chronic rhinosinusitis using the polymerase chain reaction. Otolaryngol Head Neck Surg 123, 363–367.[CrossRef]
    [Google Scholar]
  23. Kroes, I., Lepp, P. W. & Relman, D. A. ( 1999;). Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci U S A 96, 14547–14552.[CrossRef]
    [Google Scholar]
  24. Lane, D. J., Pace, B., Olsen, G. J., Stahl, D. A., Sogin, M. L. & Pace, N. R. ( 1985;). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82, 6955–6959.[CrossRef]
    [Google Scholar]
  25. Le Moal, G., Lemerre, D., Grollier, G., Desmont, C., Klossek, J. M. & Robert, R. ( 1999;). Nosocomial sinusitis with isolation of anaerobic bacteria in ICU patients. Intensive Care Med 25, 1066–1071.[CrossRef]
    [Google Scholar]
  26. Marchesi, J. R. & Weightman, A. J. ( 2000;). Modified primers facilitate rapid screening of 16S rRNA gene libraries. Biotechniques 29, 48–50.
    [Google Scholar]
  27. Marchesi, J. R., Sato, T., Weightman, A. J., Martin, T. A., Fry, J. C., Hiom, S. J., Dymock, D. & Wade, W. G. ( 1998;). Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64, 795–799.
    [Google Scholar]
  28. Melen, I., Lindahl, L., Andreasson, L. & Rundcrantz, H. ( 1986;). Chronic maxillary sinusitis.Definition, diagnosis and relation to dental infections and nasal polyposis. Acta Otolaryngol 101, 320–327.[CrossRef]
    [Google Scholar]
  29. Offenbacher, S., Jared, H. L., O'Reilly, P. G., Wells, S. R., Salvi, G. E., Lawrence, H. P., Socransky, S. S. & Beck, J. D. ( 1998;). Potential pathogenic mechanisms of periodontitis associated pregnancy complications. Ann Periodontol 3, 233–250.[CrossRef]
    [Google Scholar]
  30. Paster, B. J., Boches, S. K., Galvin, J. L., Ericson, R. E., Lau, C. N., Levanos, V. A., Sahasrabudhe, A. & Dewhirst, F. E. ( 2001;). Bacterial diversity in human subgingival plaque. J Bacteriol 183, 3770–3783.[CrossRef]
    [Google Scholar]
  31. Rajasuo, A., Jousimies-Somer, H., Savolainen, S., Leppänen, J., Murtomaa, H. & Meurman, J. H. ( 1996;). Bacteriologic findings in tonsillitis and pericoronitis. Clin Infect Dis 23, 51–60.[CrossRef]
    [Google Scholar]
  32. Rantakokko-Jalava, K., Nikkari, S., Jalava, J. & 8 other authors ( 2000;). Direct amplification of rRNA genes in diagnosis of bacterial infections. J Clin Microbiol 38, 32–39.
    [Google Scholar]
  33. Scannapieco, F. A. ( 1999;). Role of oral bacteria in respiratory infection. J Periodontol 70, 793–802.[CrossRef]
    [Google Scholar]
  34. Scannapieco, F. A., Stewart, E. M. & Mylotte, J. M. ( 1992;). Colonization of dental plaque by respiratory pathogens in medical intensive care patients. Crit Care Med 20, 740–745.[CrossRef]
    [Google Scholar]
  35. Stackebrandt, E. & Goebel, B. M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  36. Suau, A., Bonnet, R., Sutren, M., Godon, J. J., Gibson, G. R., Collins, M. D. & Dore, J. ( 1999;). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65, 4799–4807.
    [Google Scholar]
  37. van der Heijden, I. M., Wilbrink, B., Vije, A. E., Schouls, L. M., Breedveld, F. C. & Tak, P. P. ( 1999;). Detection of bacterial DNA in serial synovial samples obtained during antibiotic treatment from patients with septic arthritis. Arthritis Rheum 42, 2198–2203.[CrossRef]
    [Google Scholar]
  38. Wilson, K. H. & Blitchington, R. B. ( 1996;). Human colonic biota studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 62, 2273–2278.
    [Google Scholar]
  39. Wilson, M. J., Weightman, A. J. & Wade, W. G. ( 1997;). Applications of molecular ecology in the characterization of uncultured microorganisms associated with human disease. Rev Med Microbiol 8, 91–101.[CrossRef]
    [Google Scholar]
  40. Wilson, V. L., Tatford, B. C., Yin, X., Rajki, S. C., Walsh, M. M. & LaRock, P. ( 1999;). Species-specific detection of hydrocarbon-utilizing bacteria. J Microbiol Methods 39, 59–78.[CrossRef]
    [Google Scholar]
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