1887

Abstract

Infection with has been implicated as a potential risk factor for atherosclerosis. This study was designed to investigate the mechanisms of the anti-chlamydial activity of aspirin. A reporter gene assay for NF-κB activity, immunoblot analysis for cyclo-oxygenase (COX)-2 and radioimmunoassay for prostaglandin E (PGE) were performed. Following infection of HEp-2 cells with , NF-κB was activated, COX-2 was induced and PGE was elevated. Aspirin inhibited NF-κB activation at a concentration of 0.1 mM, partially inhibited COX-2 induction and blocked PGE synthesis completely. In addition, high doses of aspirin (1 and 2 mM) inhibited chlamydial growth in HEp-2 cells, decreasing the number and size of inclusion bodies; this effect could be overcome by adding tryptophan to the culture. Indomethacin also blocked the synthesis of PGE, but had no effect on COX-2 expression or chlamydial growth. These results indicate that aspirin not only has an anti-inflammatory activity through prevention of NF-κB activation but also has anti-chlamydial activity at high doses, possibly through depletion of tryptophan in HEp-2 cells.

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2003-05-01
2021-03-09
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