1887

Abstract

The objective of this study was to understand more of the innate immune response to by determining the expression of human β-defensin-2 (hBD-2) in various gastric mucosal tissues and MKN45 gastric cancer cells with or without . Semi-quantitative TaqMan RT-PCR and immunohistochemistry were carried out. The antimicrobial effects of a transfected hBD-2 gene against were also evaluated. The results showed that hBD-2 was expressed in inflamed gastric mucosal tissues with infection, but not in the absence of infection. Expression was also detected in gastric cancers in patients with infection. Expression was induced in the MKN45 gastric cancer cell line by in a manner dependent on the abundance of bacteria. hBD-2-transfected 3T3J2-1 cells secreted hBD-2 protein into the culture medium and this protein inhibited growth of completely. The results suggest that hBD-2 may be involved in the pathophysiology of -induced gastritis.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.04985-0
2003-01-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/jmm/52/1/41.html?itemId=/content/journal/jmm/10.1099/jmm.0.04985-0&mimeType=html&fmt=ahah

References

  1. Diamond, G. & Bevins, C. L. ( 1998;). β-Defensins: endogenous antibiotics of the innate host defense response. Clin Immun Immunopathol 88, 221–225.[CrossRef]
    [Google Scholar]
  2. Hamanaka, Y., Nakashima, M., Wada, A. & 7 other authors ( 2001;). Expression of human β-defensin 2 (hBD-2) in Helicobacter pylori induced gastritis: antibacterial effect of hBD-2 against Helicobacter pylori. Gut 49, 481–487.[CrossRef]
    [Google Scholar]
  3. Harder, J., Bartels, J., Christophers, E. & Schröder, J. M. ( 1997;). A peptide antibiotic from human skin. Nature 387, 861.[CrossRef]
    [Google Scholar]
  4. Harder, J., Bartels, J., Christophers, E. & Schröder, J. M. ( 2001;). Isolation and characterization of human β-defensin-3, a novel human inducible peptide antibiotic. J Biol Chem 276, 5707–5713.[CrossRef]
    [Google Scholar]
  5. Isomoto, H., Mizuta, Y., Miyazaki, M. & 7 other authors ( 2000;). Implication of NF-κB in Helicobacter pylori-associated gastritis. Am J Gastroenterol 95, 2768–2776.
    [Google Scholar]
  6. Japanese Research Society for Gastric Cancer (1995). Japanese Classification of Gastric Carcinoma, English edn. Tokyo: Kanehara.
  7. Marshall, B. J., Warren, J. R., Francis, G. J., Langton, S. R., Goodwin, C. S. & Blincow, E. D. ( 1987;). Rapid urease test in the management of Campylobacter pyloridis-associated gastritis. Am J Gastroenterol 82, 200–210.
    [Google Scholar]
  8. O'Neil, D. A., Cole, S. P., Martin-Porter, E., Housley, M. P., Liu, L., Ganz, T. & Kagnoff, M. F. ( 2000;). Regulation of human β-defensins by gastric epithelial cells in response to infection with Helicobacter pylori or stimulation with interleukin-1. Infect Immun 68, 5412–5415.[CrossRef]
    [Google Scholar]
  9. Schröder, J. M. & Harder, J. ( 1999;). Human β-defensin-2. Int J Biochem Cell Biol 31, 645–651.[CrossRef]
    [Google Scholar]
  10. Wada, A., Mori, N., Oishi, K. & 10 other authors ( 1999;). Induction of human β-defensin-2 mRNA expression by Helicobacter pylori in human gastric cell line MKN45 cells on cag pathogenicity island. Biochem Biophys Res Commun 263, 770–774.[CrossRef]
    [Google Scholar]
  11. Wada, A., Ogushi, K., Kimura, T. & 9 other authors ( 2001;). Helicobacter pylori-mediated transcriptional regulation of the human β-defensin 2 gene requires NF-κB. Cell Microbiol 3, 115–123.[CrossRef]
    [Google Scholar]
  12. Yajima, T., Yagihashi, A., Kameshima, H., Kobayashi, D., Furuya, D., Hirata, K. & Watanabe, N. ( 1998;). Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic detection system. Clin Chem 44, 2441–2445.
    [Google Scholar]
  13. Yoshida, Y. & Hamada, H. ( 1997;). Adenovirus-mediated inducible gene expression through tetracycline-controllable transactivator with nuclear localization signal. Biochem Biophys Res Commun 230, 426–430.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.04985-0
Loading
/content/journal/jmm/10.1099/jmm.0.04985-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error