1887

Abstract

The protective effect of DNA vaccination with the gene encoding the major outer-membrane protein (MOMP) of has been studied in non-pregnant and pregnant mouse models after chlamydial challenge. OF1 outbred mice were vaccinated intramuscularly three times every 3 weeks, mated and challenged with 2 weeks after the last injection of DNA. In non-pregnant mice, the MOMP DNA vaccine elicited a specific humoral response with predominantly IgG2a antibodies, suggesting a Th1-type immune response. The induced antibodies showed no neutralizing effect on infectivity. Moreover, immunization with the gene showed no reduction in the mean splenic bacterial counts of non-pregnant or pregnant mice or in the mean placental bacterial counts of pregnant mice after the challenge. Nevertheless, the MOMP DNA immunization induced a non-specific and partial protection in fetuses against challenge.

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2003-01-01
2021-03-08
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