1887

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Volume 61, Issue 10

The value of MALDI-TOF MS for the identification of clinically relevant anaerobic bacteria in routine laboratories Free

Abstract

Between 2010 and 2011, 283 clinically relevant non-duplicate anaerobic isolates were analysed by MALDI-TOF MS and the results were compared with conventional identification. Immediately after isolation, an ethanol precipitation was carried out on isolated colonies and the stabilized samples were anonymized and sent to the laboratory of Bruker Daltonik, Bremen, Germany, where the identification was done using the standard protocol for micro-organism identification on a Microflex LT mass spectrometer equipped with the MALDI Biotyper 3.0 software. Of 283 isolates, 218 (77 %) were identified at species level [log(score) ≥2.0], 31 isolates (10.95 %) were identified at genus level [log(score) 1.7–2.0] and 34 (12 %) gave non-reliable identification [log(score) <1.7]. Out of the 31 isolates with log(score) 1.7–2.0, in the case of 24 isolates the species name given by the MALDI Biotyper was accepted if it was the same as for the classical identification. Of 218 isolates identified at species level, 40 results were discordant with phenotypic identification, and of the 31 isolates identified at genus level according to the manufacturer’s score cut-off, four gave results discordant with the phenotypic method. For the 44 discordant results, 16S rRNA gene sequencing confirmed MALDI-TOF MS identification in 41 cases, leaving three isolates (0.7 %) that had been misidentified by MALDI-TOF MS.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-10-01
2024-04-26
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References

  1. Chassard C., Delmas E., Lawson P. A., Bernalier-Donadille A. 2008; Bacteroides xylanisolvens sp. nov., a xylan-degrading bacterium isolated from human faeces. Int J Syst Evol Microbiol 58:1008–1013 [View Article][PubMed]
     [Google Scholar]
  2. Citron D. M. 2012; Pre-molecular identification: ignorance was bliss?. Anaerobe 18:189–191 [View Article][PubMed]
     [Google Scholar]
  3. Downes J., King A., Hardie J., Phillips I. 1999; Evaluation of the Rapid ID 32A system for identification of anaerobic Gram-negative bacilli, excluding the Bacteroides fragilis group. Clin Microbiol Infect 5:319–326 [View Article][PubMed]
     [Google Scholar]
  4. Eigner U., Holfelder M., Oberdorfer K., Betz-Wild U., Bertsch D., Fahr A. M. 2009; Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory. Clin Lab 55:289–296[PubMed]
     [Google Scholar]
  5. Fedorko D. P., Drake S. K., Stock F., Murray P. R. 2012; Identification of clinical isolates of anaerobic bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Eur J Clin Microbiol Infect Dis 31:2257–2262 [CrossRef]
     [Google Scholar]
  6. Hedberg M., Nord C. E. ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria 2003; Antimicrobial susceptibility of Bacteroides fragilis group isolates in Europe. Clin Microbiol Infect 9:475–488 [View Article][PubMed]
     [Google Scholar]
  7. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
     [Google Scholar]
  8. Jousimies-Somer H. R., Summanen P. 2002b; Recent taxonomic changes and terminology update of clinically significant anaerobic Gram-negative bacteria (excluding spirochetes). Clin Infect Dis 35:Suppl. 1S17–S21 [View Article][PubMed]
     [Google Scholar]
  9. Jousimies-Somer H. R., Summanen P., Citron D. M., Baron E. J., Wexler H. M., Finegold S. M. editors 2002a Wadsworth-KTL Anaerobic Bacteriology Manual, 6th edn. Belmont, CA: Star Publishing Company;
     [Google Scholar]
  10. Justesen U. S., Skov M. N., Knudsen E., Holt H. M., Søgaard P., Justesen T. 2010; 16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures. J Clin Microbiol 48:946–948 [View Article][PubMed]
     [Google Scholar]
  11. Justesen U. S., Holm A., Knudsen E., Andersen L. B., Jensen T. G., Kemp M., Skov M. N., Gahrn-Hansen B., Møller J. K. 2011; Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. J Clin Microbiol 49:4314–4318 [View Article][PubMed]
     [Google Scholar]
  12. La Scola B., Fournier P. E., Raoult D. 2011; Burden of emerging anaerobes in the MALDI-TOF and 16S rRNA gene sequencing era. Anaerobe 17:106–112 [View Article][PubMed]
     [Google Scholar]
  13. Lau S. K., Ng K. H., Woo P. C., Yip K. T., Fung A. M., Woo G. K., Chan K. M., Que T. L., Yuen K. Y. 2006; Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures. J Clin Pathol 59:219–222 [View Article][PubMed]
     [Google Scholar]
  14. Lee E. H. L., Degener J. E., Welling G. W., Veloo A. C. M. 2011; Evaluation of the Vitek 2 ANC card for identification of clinical isolates of anaerobic bacteria. J Clin Microbiol 49:1745–1749 [View Article][PubMed]
     [Google Scholar]
  15. Nagy E., Maier T., Urban E., Terhes G., Kostrzewa M. ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria 2009; Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. Clin Microbiol Infect 15:796–802 [View Article][PubMed]
     [Google Scholar]
  16. Nagy E., Becker S., Sóki J., Urbán E., Kostrzewa M. 2011a; Differentiation of division I (cfiA-negative) and division II (cfiA-positive) Bacteroides fragilis strains by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Med Microbiol 60:1584–1590 [View Article][PubMed]
     [Google Scholar]
  17. Nagy E., Urbán E., Nord C.-E. ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria 2011b; Antimicrobial susceptibility of Bacteroides fragilis group isolates in Europe: 20 years of experience. Clin Microbiol Infect 17:371–379 [View Article][PubMed]
     [Google Scholar]
  18. Phillips I., King A., Nord C. E., Hoffstedt B. European Study Group 1992; Antibiotic sensitivity of the Bacteroides fragilis group in Europe. Eur J Clin Microbiol Infect Dis 11:292–304 [View Article][PubMed]
     [Google Scholar]
  19. Seng P., Drancourt M., Gouriet F., La Scola B., Fournier P.-E., Rolain J. M., Raoult D. 2009; Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49:543–551 [View Article][PubMed]
     [Google Scholar]
  20. Shah H. N., Keys C. J., Schmid O., Gharbia S. E. 2002) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and proteomics: a new era in anaerobic microbiology. Clin Infect Dis 35:Suppl. 158–64 [CrossRef]
     [Google Scholar]
  21. Simmon K. E., Mirrett S., Reller L. B., Petti C. A. 2008; Genotypic diversity of anaerobic isolates from bloodstream infections. J Clin Microbiol 46:1596–1601 [View Article][PubMed]
     [Google Scholar]
  22. Snydman D. R., Jacobus N. V., McDermott L. A., Ruthazer R., Golan Y., Goldstein E. J., Finegold S. M., Harrell L. J., Hecht D. W. other authors 2007; National survey on the susceptibility of Bacteroides fragilis group: report and analysis of trends in the United States from 1997 to 2004. Antimicrob Agents Chemother 51:1649–1655 [View Article][PubMed]
     [Google Scholar]
  23. Song Y., Liu Ch., Bolanos M., Lee J., McTeague M., Finegold S. M. 2005; Evaluation of 16S rRNA sequencing and reevaluation of a short biochemical scheme for identification of clinically significant Bacteroides species. J Clin Microbiol 43:1531–1537 [View Article][PubMed]
     [Google Scholar]
  24. Veloo A. C. M., Erhard M., Welker M., Welling G. W., Degener J. E. 2011; Identification of Gram-positive anaerobic cocci by MALDI-TOF mass spectrometry. Syst Appl Microbiol 34:58–62 [View Article][PubMed]
     [Google Scholar]
  25. Wybo I., De Bel A., Soetens O., Echahidi F., Vandoorslaer K., Van Cauwenbergh M., Piérard D. 2011; Differentiation of cfiA-negative and cfiA-positive Bacteroides fragilis isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 49:1961–1964 [View Article][PubMed]
     [Google Scholar]
  26. Wybo I., Soetens O., De Bel A., Echahidi F., Vancutsem E., Vandoorslaer K., Piérard D. 2012; Species identification of clinical Prevotella isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 50:1415–1418 [View Article][PubMed]
     [Google Scholar]
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The value of MALDI-TOF MS for the identification of clinically relevant anaerobic bacteria in routine laboratories
J. Med. Microbiol. 61, 1393 (2012); https://doi.org/10.1099/jmm.0.043927-0
/content/journal/jmm/10.1099/jmm.0.043927-0
/content/journal/jmm/10.1099/jmm.0.043927-0
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