1887

Abstract

A real-time PCR assay targeting the gene was developed to detect [Lancefield group B (GBS)] from clinical samples. A total of 110 blood culture-negative samples [75 cerebrospinal fluid (CSF) and 35 EDTA blood samples] from neonates with probable GBS sepsis or meningitis were analysed. Among these, 16 of the 75 CSF samples were positive [21.3 %, 95 % confidence interval (CI) 12.7–32.3 %] and two of the 35 EDTA blood samples were positive (5.7 %, 95 % CI 0.7–19.2 %). The proportion testing positive in the CSF samples was significantly higher than in the EDTA blood samples ( = 0.05, Fisher’s exact test). Overall, this real-time PCR assay was shown to be superior to culture methods for detection of GBS from CSF and EDTA blood samples.

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2012-08-01
2021-10-24
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