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Volume 61, Issue 5

Characterization of a USA300 protein signature using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Free

Abstract

strain type USA300 is an important human pathogen. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has been used successfully for rapid identification of at the genus and species levels. Here, it was hypothesized that MALDI-TOF could be used to identify USA300 organisms at the strain level. A genetic algorithm model using ClinProTools software (Bruker Daltonics) was built using 47 isolates of USA300 and 77 non-USA300 isolates. Three mass/charge peaks (5932, 6423 and 6592) were found to be discriminators between the groups of isolates. The model was validated using 224 test isolates: 197 of 224 test isolates were correctly classified as ‘USA300 family’ or ‘non-USA300’. The sensitivity of the model was 0.87, with a specificity of 0.89, positive likelihood ratio of 8.19 and negative likelihood ratio of 0.15. The three-peak intensity MALDI-TOF model designed and tested in the current study can be used to rapidly identify USA300 family isolates with reasonable sensitivity and specificity.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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/content/journal/jmm/10.1099/jmm.0.037978-0
2012-05-01
2023-05-29
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Characterization of a Staphylococcus aureus USA300 protein signature using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
J. Med. Microbiol. 61, 640 (2012); https://doi.org/10.1099/jmm.0.037978-0
/content/journal/jmm/10.1099/jmm.0.037978-0
/content/journal/jmm/10.1099/jmm.0.037978-0
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