1887

Abstract

The group encompasses three species: , and . These species are phenotypically indistinguishable, and molecular methods are needed for their detection. We analysed 152 unique blood culture isolates of the group obtained during 1997–2011. The isolates were screened by PCR amplification of the gene encoding secondary alcohol dehydrogenase, followed by digestion with the restriction enzyme I. Isolates with RFLP patterns distinct from those of the group were characterized as (90.8 %), (8.6 %) and (0.6 %). Antifungal susceptibility tests indicated that all isolates were susceptible to itraconazole, amphotericin B and caspofungin. Although and isolates were susceptible to fluconazole, higher MICs (≥2 mg l) were observed for . Three isolates (2.0 %) of were resistant to voriconazole. Five C. isolates (3.3 %) were intermediate, and a single isolate (0.7 %) was resistant (MIC 16 mg l) to fluconazole. These data were confirmed using reference strains. It was observed that isolates were less susceptible to all triazoles, and this finding deserves further attention to assess the appearance of cross-resistance phenomena. In conclusion, and are involved in a small but significant number of invasive infections in Brazil.

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2012-07-01
2019-10-17
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