1887

Abstract

In this study, the frequency of extended-spectrum β-lactamases (ESBL) and plasmid-mediated quinolone resistance (PMQR) mechanisms were investigated in 206 clinical isolates of third-generation cephalosporin (3GC)-resistant in four hospitals in the Republic of Ireland. was the predominant ESBL gene. Of these 3GC resistant isolates, 54 % were also resistant to ciprofloxacin. Investigation of the PMQR mechanisms revealed that the gene predominated in fluoroquinolone-resistant (FQR) strains of and , while the gene predominated in the FQR strains of . The gene was frequently identified with the gene but was not always on the same plasmid. The prevalence of the gene appeared to be hospital-dependent. The epidemiology of both ESBL-producing and PMQR strains within the four hospitals indicated that their prevalence is not due to the spread of these resistance genes between isolates from different hospitals.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.035246-0
2012-01-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/61/1/142.html?itemId=/content/journal/jmm/10.1099/jmm.0.035246-0&mimeType=html&fmt=ahah

References

  1. Bonnet R.. ( 2004;). Growing group of extended-spectrum β-lactamases: the CTX-M enzymes. . Antimicrob Agents Chemother 48:, 1–14. [CrossRef][PubMed]
    [Google Scholar]
  2. Boyd D. A., Tyler S., Christianson S., McGeer A., Muller M. P., Willey B. M., Bryce E., Gardam M., Nordmann P., Mulvey M. R.. ( 2004;). Complete nucleotide sequence of a 92-kilobase plasmid harboring the CTX-M-15 extended-spectrum β-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada. . Antimicrob Agents Chemother 48:, 3758–3764. [CrossRef][PubMed]
    [Google Scholar]
  3. Cantón R., Novais A., Valverde A., Machado E., Peixe L., Baquero F., Coque T. M.. ( 2008;). Prevalence and spread of extended-spectrum β-lactamase-producing Enterobacteriaceae in Europe. . Clin Microbiol Infect 14: (Suppl 1), 144–153. [CrossRef][PubMed]
    [Google Scholar]
  4. CLSI ( 2007;). Performance Standards for Antimicrobial Susceptibility Testing: Seventeenth Informational Supplement. M100–S17. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  5. Colom K., Pérez J., Alonso R., Fernández-Aranguiz A., Lariño E., Cisterna R.. ( 2003;). Simple and reliable multiplex PCR assay for detection of blaTEM, blaSHV and blaOXA-1 genes in Enterobacteriaceae. . FEMS Microbiol Lett 223:, 147–151. [CrossRef][PubMed]
    [Google Scholar]
  6. EARS-Net ( 2010;). Antimicrobial resistance surveillance in Europe 2009. Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net). . Stockholm:: ECDC;; 2010. ISBN 978-92-9193-227-6.
  7. Gay K., Robicsek A., Strahilevitz J., Park C. H., Jacoby G., Barrett T. J., Medalla F., Chiller T. M., Hooper D. C.. ( 2006;). Plasmid-mediated quinolone resistance in non-Typhi serotypes of Salmonella enterica. . Clin Infect Dis 43:, 297–304. [CrossRef][PubMed]
    [Google Scholar]
  8. Mac Aogáin M., Mooij M. J., Adams C., Clair J., O’Gara F.. ( 2010;). Emergence of extended-spectrum β-lactamase and fluoroquinolone resistance genes among Irish multidrug-resistant isolates. . Diagn Microbiol Infect Dis 67:, 106–109. [CrossRef][PubMed]
    [Google Scholar]
  9. Munday C. J., Boyd D. A., Brenwald N., Miller M., Andrews J. M., Wise R., Mulvey M. R., Hawkey P. M.. ( 2004;). Molecular and kinetic comparison of the novel extended-spectrum β-lactamases CTX-M-25 and CTX-M-26. . Antimicrob Agents Chemother 48:, 4829–4834. [CrossRef][PubMed]
    [Google Scholar]
  10. Robicsek A., Strahilevitz J., Jacoby G. A., Macielag M., Abbanat D., Park C. H., Bush K., Hooper D. C.. ( 2006;). Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase. . Nat Med 12:, 83–88. [CrossRef][PubMed]
    [Google Scholar]
  11. Walsh F., Willcock J., Amyes S.. ( 2003;). High-level telithromycin resistance in laboratory-generated mutants of Streptococcus pneumoniae. . J Antimicrob Chemother 52:, 345–353. [CrossRef][PubMed]
    [Google Scholar]
  12. Woodford N., Fagan E. J., Ellington M. J.. ( 2006;). Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum β-lactamases. . J Antimicrob Chemother 57:, 154–155. [CrossRef][PubMed]
    [Google Scholar]
  13. Woodford N., Carattoli A., Karisik E., Underwood A., Ellington M. J., Livermore D. M.. ( 2009;). Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25 : H4-ST131 clone. . Antimicrob Agents Chemother 53:, 4472–4482. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.035246-0
Loading
/content/journal/jmm/10.1099/jmm.0.035246-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error