1887

Abstract

is one of the oral pathogens associated with infective endocarditis (IE). With respect to bacterial binding ability to the extracellular matrix, the Cnm protein, a cell surface collagen-binding adhesin of , is known as one of the possible virulence factors with regard to IE. In this study, we aimed to determine the distribution of the gene, which encodes Cnm, in a large number of clinical isolates of from Thai subjects. Then, the -positive strains were classified using a multilocus sequence typing (MLST) scheme, which we constructed previously. In addition, the data were analysed together with our previous MLST data of -positive strains from Japan and Finland in order to evaluate the clonal relationship among strains harbouring the gene. The gene was detected in 12.4 % of all 750 Thai isolates, and serotype f showed the highest rate of detection (54.5 %). According to the MLST data, two clonal complex groups were revealed as the important clones related to -positive from various origins of isolation. Moreover, the collagen-binding properties of strains with the gene were significantly greater than those of strains without the gene, although four -negative strains classified into two sequence types (STs), ST110 and ST136, showed extremely high collagen-binding rates suggesting the presence of additional genes involved with collagen binding in these STs. Taken together, these results provided information on both epidemiological as well as evolutional aspects of possessing the gene.

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2011-11-01
2019-10-23
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