1887

Abstract

The development of effective strategies to prevent urinary tract infections (UTIs) has become an important goal in public health. is commonly associated with complicated UTIs and expresses several virulence factors, including mannose-resistant -like (MR/P) fimbriae. Here, a fusion protein formed from MrpA, the structural protein of MR/P fimbriae, and TetC, a non-toxic but highly immunogenic fragment of tetanus toxin, to be delivered by an attenuated Typhimurium mutant was constructed. The ability of this strain to induce an immune response and to protect mice against a urinary tract challenge with was investigated. The protein was successfully expressed in Typhimurium. After two immunization doses, intra-nasally vaccinated mice showed a significant increase in specific serum IgG against MrpA and against lipopolysaccharide, as well as a significant decrease in kidney and bladder colonization by after challenge. However, no significant correlation was observed between antibody response and kidney or bladder colonization. MrpA fused to TetC and expressed in Typhimurium effectively protected mice against an experimental UTI.

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2011-07-01
2019-10-15
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