1887

Abstract

Leishmaniasis is a parasitic disease affecting over 12 million individuals worldwide. As current treatments are insufficient, the development of an effective vaccine is a priority. This study generated and assessed the efficacy of vaccines engineered from the non-colonizing, non-pathogenic Gram-positive bacterium . A truncated, codon-optimized version of the A2 antigen from was engineered for expression in in three different subcellular compartments: in the cytoplasm, secreted outside the cell or anchored to the cell wall. These three A2-expressing strains were tested for their ability to generate A2-specific immune responses and as live vaccines against visceral infection in BALB/c mice. Subcutaneous immunization with live expressing A2 anchored to the cell wall effectively induced high levels of antigen-specific serum antibodies. It was demonstrated that -based vaccines are a feasible approach in the generation of live vaccines against leishmaniasis. The strains generated in this study provide an excellent foundation for further studies on live bacterial vaccines against leishmaniasis and other pathogens.

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2011-09-01
2019-10-16
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