1887

Abstract

The loop-mediated isothermal amplification (LAMP) assay detecting the gene of sequence type gc8 () was established for the identification of a hypervirulent strain, PCR ribotype 027. Of 107 isolates examined, 27 belonging to PCR ribotype 027 were all positive for the LAMP assay. The remaining 80 isolates were typed into 47 different PCR ribotypes other than type 027, and were negative for the LAMP assay with the exception of two isolates. The sensitivity and specificity of the LAMP method for identification of PCR ribotype 027 were 100 % and 98 %, respectively. The LAMP assay detecting is a reliable tool for the identification of PCR ribotype 027 . This simple and rapid method will contribute to detection of the hypervirulent strain.

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2011-08-01
2019-10-20
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