1887

Abstract

is an important opportunistic human fungal pathogen. Infections caused by are related to the formation of a biofilm. The biofilm enhances the resistance of the defence system, increases its resistance to antifungal drugs and induces increased drug tolerance, making clinical care more challenging. The activity of -2-dodecenoic acid (BDSF; a diffusible signal factor from ) and -2-dodecenoic acid (-BDSF) against growth, germ-tube germination and biofilm formation was estimated by absorbance measurements and microscopic assessments. biofilms were prepared using a static microtitre plate model. Quantitative analysis of biofilm formation was performed using a 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide reduction assay to evaluate the effect of different concentrations of BDSF and -BDSF at different stages of biofilm formation. Reductions in biofilm structure and formation were visualized by inverted microscopy. Real-time RT-PCR was employed to estimate the mRNA expression levels of the hyphae-specific genes and . It was found that 30 µM of either BDSF or -BDSF reduced germ-tube formation by approximately 70 % without inhibiting yeast growth. Yeast growth was strongly repressed by the exogenous addition of 300 µM BDSF and -BDSF at 0 and 1 h after cell attachment, with biofilm formation being reduced by approximately 90 and 60 %, respectively. BDSF and -BDSF were more effective against biofilm formation than farnesol and the diffusible signal factor -11-methyl-2-dodecenoic acid. None of the four drugs was able to destroy pre-formed biofilms. Real-time RT-PCR analysis showed that was downregulated by approximately 90 % and was downregulated by 70–80 % by 60 µM BDSF and -BDSF, implying that BDSF and -BDSF block biofilm formation by interfering with the morphological switch. These results suggest that BDSF and -BDSF are potentially useful therapeutic agents worthy of further study.

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2011-11-01
2020-08-04
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References

  1. Argimón S., Wishart J. A., Leng R., Macaskill S., Mavor A., Alexandris T., Nicholls S., Knight A. W., Enjalbert B. et al. 2007; Developmental regulation of an adhesin gene during cellular morphogenesis in the fungal pathogen Candida albicans. Eukaryot Cell 6:682–692 [CrossRef][PubMed]
    [Google Scholar]
  2. Boon C., Deng Y., Wang L.-H., He Y., Xu J.-L., Fan Y., Pan S. Q., Zhang L.-H. 2008; A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition. ISME J 2:27–36 [CrossRef][PubMed]
    [Google Scholar]
  3. Borecká-Melkusová S., Moran G. P., Sullivan D. J., Kucharíková S., Chorvát D. Jr, Bujdáková H. 2009; The expression of genes involved in the ergosterol biosynthesis pathway in Candida albicans and Candida dubliniensis biofilms exposed to fluconazole. Mycoses 52:118–128 [CrossRef][PubMed]
    [Google Scholar]
  4. Brown A. J. P., Gow N. A. R. 1999; Regulatory networks controlling Candida albicans morphogenesis. Trends Microbiol 7:333–338 [CrossRef][PubMed]
    [Google Scholar]
  5. Brown D. H. Jr, Giusani A. D., Chen X., Kumamoto C. A. 1999; Filamentous growth of Candida albicans in response to physical environmental cues and its regulation by the unique CZF1 gene. Mol Microbiol 34:651–662 [CrossRef][PubMed]
    [Google Scholar]
  6. Cao Y.-Y., Cao Y.-B., Xu Z., Ying K., Li Y., Xie Y., Zhu Z.-Y., Chen W.-S., Jiang Y.-Y. 2005; cDNA microarray analysis of differential gene expression in Candida albicans biofilm exposed to farnesol. Antimicrob Agents Chemother 49:584–589 [CrossRef][PubMed]
    [Google Scholar]
  7. Chandra J., Kuhn D. M., Mukherjee P. K., Hoyer L. L., McCormick T., Ghannoum M. A. 2001; Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance. J Bacteriol 183:5385–5394 [CrossRef][PubMed]
    [Google Scholar]
  8. Costerton J. W. 1995; Overview of microbial biofilms. J Ind Microbiol 15:137–140 [CrossRef][PubMed]
    [Google Scholar]
  9. da Silva W. J., Seneviratne J., Parahitiyawa N., Rosa E. A. R., Samaranayake L. P., Del Bel Cury A. A. 2008; Improvement of XTT assay performance for studies involving Candida albicans biofilms. Braz Dent J 19:364–369[PubMed]
    [Google Scholar]
  10. Davies D. G., Marques C. N. H. 2009; A fatty acid messenger is responsible for inducing dispersion in microbial biofilms. J Bacteriol 191:1393–1403 [CrossRef][PubMed]
    [Google Scholar]
  11. Davies D. G., Parsek M. R., Pearson J. P., Iglewski B. H., Costerton J. W., Greenberg E. P. 1998; The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280:295–298 [CrossRef][PubMed]
    [Google Scholar]
  12. Douglas L. J. 2003; Candida biofilms and their role in infection. Trends Microbiol 11:30–36 [CrossRef][PubMed]
    [Google Scholar]
  13. Green C. B., Cheng G., Chandra J., Mukherjee P., Ghannoum M. A., Hoyer L. L. 2004; RT-PCR detection of Candida albicans ALS gene expression in the reconstituted human epithelium (RHE) model of oral candidiasis and in model biofilms. Microbiology 150:267–275 [CrossRef][PubMed]
    [Google Scholar]
  14. Henriques M., Martins M., Azeredo J., Oliveira R. 2007; Effect of farnesol on Candida dubliniensis morphogenesis. Lett Appl Microbiol 44:199–205 [CrossRef][PubMed]
    [Google Scholar]
  15. Hogan D. A., Vik A., Kolter R. 2004; A Pseudomonas aeruginosa quorum-sensing molecule influences Candida albicans morphology. Mol Microbiol 54:1212–1223 [CrossRef][PubMed]
    [Google Scholar]
  16. Hornby J. M., Jensen E. C., Lisec A. D., Tasto J. J., Jahnke B., Shoemaker R., Dussault P., Nickerson K. W. 2001; Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol. Appl Environ Microbiol 67:2982–2992 [CrossRef][PubMed]
    [Google Scholar]
  17. Hoyer L. L., Green C. B., Oh S.-H., Zhao X. 2008; Discovering the secrets of the Candida albicans agglutinin-like sequence (ALS) gene family – a sticky pursuit. Med Mycol 46:1–15 [CrossRef][PubMed]
    [Google Scholar]
  18. Kuhn D. M., Balkis M., Chandra J., Mukherjee P. K., Ghannoum M. A. 2003; Uses and limitations of the XTT assay in studies of Candida growth and metabolism. J Clin Microbiol 41:506–508 [CrossRef][PubMed]
    [Google Scholar]
  19. Kumamoto C. A. 2002; Candida biofilms. Curr Opin Microbiol 5:608–611 [CrossRef][PubMed]
    [Google Scholar]
  20. Lo H.-J., Köhler J. R., DiDomenico B., Loebenberg D., Cacciapuoti A., Fink G. R. 1997; Nonfilamentous C. albicans mutants are avirulent. Cell 90:939–949 [CrossRef][PubMed]
    [Google Scholar]
  21. Miller M. B., Bassler B. L. 2001; Quorum sensing in bacteria. Annu Rev Microbiol 55:165–199 [CrossRef][PubMed]
    [Google Scholar]
  22. Molero G., Díez-Orejas R., Navarro-García F., Monteoliva L., Pla J., Gil C., Sánchez-Pérez M., Nombela C. 1998; Candida albicans: genetics, dimorphism and pathogenicity. Int Microbiol 1:95–106[PubMed]
    [Google Scholar]
  23. Parsek M. R., Greenberg E. P. 1999; Quorum sensing signals in development of Pseudomonas aeruginosa biofilms. Methods Enzymol 310:43–55 [CrossRef][PubMed]
    [Google Scholar]
  24. Pierce C. G., Thomas D. P., López-Ribot J. L. 2009; Effect of tunicamycin on Candida albicans biofilm formation and maintenance. J Antimicrob Chemother 63:473–479 [CrossRef][PubMed]
    [Google Scholar]
  25. Ramage G., Wickes B. L., Lopez-Ribot J. L. 2001a; Biofilms of Candida albicans and their associated resistance to antifungal agents. Am Clin Lab 20:42–44[PubMed]
    [Google Scholar]
  26. Ramage G., Vandewalle K., Wickes B. L., López-Ribot J. L. 2001b; Characteristics of biofilm formation by Candida albicans. Rev Iberoam Micol 18:163–170[PubMed]
    [Google Scholar]
  27. Ramage G., Saville S. P., Wickes B. L., López-Ribot J. L. 2002a; Inhibition of Candida albicans biofilm formation by farnesol, a quorum-sensing molecule. Appl Environ Microbiol 68:5459–5463 [CrossRef][PubMed]
    [Google Scholar]
  28. Ramage G., Bachmann S., Patterson T. F., Wickes B. L., López-Ribot J. L. 2002b; Investigation of multidrug efflux pumps in relation to fluconazole resistance in Candida albicans biofilms. J Antimicrob Chemother 49:973–980 [CrossRef][PubMed]
    [Google Scholar]
  29. Sharkey L. L., McNemar M. D., Saporito-Irwin S. M., Sypherd P. S., Fonzi W. A. 1999; HWP1 functions in the morphological development of Candida albicans downstream of EFG1, TUP1, and RBF1. J Bacteriol 181:5273–5279[PubMed]
    [Google Scholar]
  30. Singh P. K., Schaefer A. L., Parsek M. R., Moninger T. O., Welsh M. J., Greenberg E. P. 2000; Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms. Nature 407:762–764 [CrossRef][PubMed]
    [Google Scholar]
  31. Staab J. F., Sundstrom P. 1998; Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans. Yeast 14:681–686 [CrossRef][PubMed]
    [Google Scholar]
  32. Staab J. F., Bahn Y.-S., Tai C.-H., Cook P. F., Sundstrom P. 2004; Expression of transglutaminase substrate activity on Candida albicans germ tubes through a coiled, disulfide-bonded N-terminal domain of Hwp1 requires C-terminal glycosylphosphatidylinositol modification. J Biol Chem 279:40737–40747 [CrossRef][PubMed]
    [Google Scholar]
  33. Stephens C. 2002; Microbiology: breaking down biofilms. Curr Biol 12:R132–R134 [CrossRef][PubMed]
    [Google Scholar]
  34. Wang L. H. 2006 Inhibitors of Yeast Filamentous Growth and Method of Their Manufacture Singapore: USPA Publication, Agency for Science, Technology and Research;
    [Google Scholar]
  35. Wang L.-H., He Y., Gao Y., Wu J. E., Dong Y.-H., He C., Wang S. X., Weng L.-X., Xu J.-L. et al. 2004; A bacterial cell–cell communication signal with cross-kingdom structural analogues. Mol Microbiol 51:903–912 [CrossRef][PubMed]
    [Google Scholar]
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