1887

Abstract

This study provides guidelines on the usefulness of full and 527 bp 16S rRNA gene sequencing and Microseq databases for identifying medically important aerobic Gram-negative bacteria. Overall, full and 527 bp 16S rRNA gene sequencing can identify 26.1 % and 32.6 %, respectively, of medically important aerobic Gram-negative bacteria confidently to the species level, whereas the full-MicroSeq and 500-MicroSeq databases can identify 15.2 % and 26.1 %, respectively, of medically important aerobic Gram-negative bacteria confidently to the species level. Among the major groups of aerobic Gram-negative bacteria, the methods and databases are least useful for identification of , and species. None of the species can be confidently or doubtfully identified, whereas only 0 % and 0–33.3 % of species and 0–10 % and 0–10 % of species can be confidently and doubtfully identified, respectively. On the other hand, these methods and databases are most useful for identification of members of the families and and species: 29.6–59.3 % and 7.4–18.5 % of members of , 36–52 % and 12–24 % of members of , and 26.7–60 % and 0–13.3 % of species can be confidently and doubtfully identified, respectively. Thirty-nine medically important aerobic Gram-negative bacteria that should be confidently identified by full 16S rRNA gene sequencing are not included in the full-MicroSeq database. Twenty-three medically important aerobic Gram-negative bacteria that should be confidently identified by 527 bp 16S rRNA gene sequencing are not included in the 500-MicroSeq database. Compared with results of our previous studies on anaerobic and Gram-positive bacteria, full and 527 bp 16S rRNA gene sequencing are able to confidently identify significantly more anaerobic Gram-positive and Gram-negative bacteria than aerobic Gram-positive and Gram-negative bacteria.

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2011-09-01
2019-12-05
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vol. , part 9, pp. 1281 - 1286

[PDF](167 kb), including: Percentages of different groups of medically important aerobic Gram-negative bacteria that can be successfully identified by full and 527 bp 16S rRNA gene sequencing using different cutoff values. Usefulness of full 16S rRNA sequence, 527-bp 16S rRNA sequence and the MicroSeq databases for identification of medically important , , (members of , , , , , and ), (members of ), (members of ), (members of ), (members of and ) and .



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