1887

Abstract

The genus contains a number of yeast species which are opportunistic pathogens and are associated with life-threatening infections in immunocompromised individuals. Provision of appropriate therapy relies on the rapid identification of the infecting species, and existing methods of identifying species in clinical samples are time and resource intensive and are not always specific enough to differentiate between drug-susceptible and drug-resistant species. We have previously developed a system for the rapid detection of yeast pathogens in clinical samples using PCR followed by hybridization with a suite of five species-specific, electrochemically labelled DNA probes. The limit of detection of the assay was shown to be 37 fg (∼1 genome) per reaction using extracted genomic DNA. We carried out a study to test the limit of detection of one of the probes, CA PR3, using blood samples from a healthy donor that were spiked with genomic DNA or with cells. Our results demonstrated a limit of detection of 37 fg (ml blood) (∼1 genome ml) using extracted DNA or 10 c.f.u. (ml blood) using cells, indicating that the assay is capable of detecting nucleic acid at levels that are encountered in clinical samples.

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2011-04-01
2019-12-09
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