1887

Abstract

This study evaluated a multiplex real-time PCR method specific for the , - and - genes for rapid identification of , and non- coagulase-negative staphylococci (CoNS), and meticillin susceptibility testing directly in positive blood cultures that grew Gram-positive cocci in clusters. A total of 100 positive blood cultures produced: 39 [12 meticillin-resistant (MRSA), 31 % of all the ]; 30 (56.6 % of the CoNS), 8 (15.1 %), 3 (5.7 %), 4 (7.5 %), 3 (5.7 %), 2 (3.8 %), 1 (1.9 %) and 2 unidentified spp. (3.8 %); and 1 in pure culture. Two blood cultures had no growth on subculture and five blood cultures grew mixed CoNS. For the 95 blood cultures with pure growth or no growth on subculture, there was very good agreement between real-time PCR and the BD Phoenix identification system for staphylococcal species categorization in , and non- CoNS and meticillin-resistance determination (Cohen's unweighted kappa coefficient =0.882). All MRSA and meticillin-susceptible were correctly identified by amplification. PCR amplification of was more sensitive for direct detection of meticillin-resistant CoNS in positive blood cultures than testing with the BD Phoenix system. There were no major errors when identifying staphylococcal isolates and their meticillin susceptibility within 2.5 h. Further studies are needed to evaluate the clinical benefit of using such a rapid test on the consumption of glycopeptide antibiotics and the alteration of empiric therapy in the situation of positive blood cultures growing staphylococci, and the respective clinical outcomes.

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2010-12-01
2019-10-16
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