1887

Abstract

In this study, PCR–RFLP analysis (PRA) targeting and gene regions was evaluated for the identification of mycobacterial species isolated from Malaysian patients. Overall, the PRA identified 92.2 % of 90 isolates compared to 85.6 % by the PRA. With 47 rapidly growing species, the PRA identified fewer (89.4 %) species than the PRA (95.7 %), but with 23 slow-growing species the reverse was true (91.3 % identification by the PRA but only 52.5 % by the PRA). There were 16 isolates with discordant PRA results, which were resolved by 16S rRNA and gene sequence analysis. The findings in this study suggest that the PRA is more useful than the PRA for the identification of species, particularly with the slow-growing members of the genus. In addition, this study reports 5 and 12 novel restriction patterns for inclusion in the and PRA algorithms, respectively.

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2010-11-01
2019-11-12
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vol. , part 11, pp. 1311 - 1316

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Identification of mycobacterial isolates with discordant and PRA results by growth and biochemical characteristics and DNA sequence analysis.



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