1887

Abstract

is a food-borne intracellular pathogen that mainly infects pregnant and immunocompromised individuals. The pore-forming haemolysin listeriolysin O (LLO), the main virulence factor of , allows bacteria to escape from the harsh environment of the phagosome to the cytoplasm of the infected cell. This leads to processing of bacterial antigens predominantly through the cytosolic MHC class I presentation pathway. We previously engineered the food-grade bacterium to express LLO and demonstrated an LLO-specific CD8 response upon immunization of mice with the engineered vaccine strains. In the present work, we examined the immune response and protective efficacy of an strain co-expressing LLO and a truncated form of the listerial P60 antigen (tP60). Oral immunization revealed no significant protection against listeriosis with expressing LLO, tP60 or the combined LLO/tP60. In contrast, intraperitoneal vaccination induced an LLO-specific CD8 immune response with LLO-expressing but no significant improvement in protection was observed following vaccination with the combined LLO/tP60 expressing strain. This may be due to the low level of tP60 expression in the LLO/tP60 strain. These results demonstrate the necessity for improved oral vaccination strategies using LLO-expressing vaccine vectors.

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2010-08-01
2019-10-18
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