1887

Abstract

To estimate the invasive disease potential of serotypes, invasive isolates (=138) were compared with nasopharyngeal isolates (=153) from children under 6 years of age in the Czech Republic. Odds ratios (ORs) based on a comparison of the distribution of serotypes amongst invasive and carriage isolates were calculated for individual serotypes and 172 strains were characterized using multilocus sequence typing. The ORs of serotypes 9V and 14 were significantly greater than 1, suggesting an association with invasive disease, while serotypes 6A and 23F were significantly associated with carriage (ORs less than 1). A single predominant clone with high invasive disease potential was found in each of the 9V, 7F, 14 and 1 serotypes while carriage-associated serotypes were highly heterogeneous.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.018390-0
2010-09-01
2019-11-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/59/9/1079.html?itemId=/content/journal/jmm/10.1099/jmm.0.018390-0&mimeType=html&fmt=ahah

References

  1. Bratcher, P. E., Park, I. H., Hollingshead, S. K. & Nahm, M. H. ( 2009; ). Production of a unique pneumococcal capsule serotype belonging to serogroup 6. Microbiology 155, 576–583.[CrossRef]
    [Google Scholar]
  2. Brueggemann, A. B., Griffiths, D. T., Meats, E., Peto, T., Crook, D. W. & Spratt, B. G. ( 2003; ). Clonal relationships between invasive and carriage Streptococcus pneumoniae and serotype- and clone-specific differences in invasive disease potential. J Infect Dis 187, 1424–1432.[CrossRef]
    [Google Scholar]
  3. Brueggemann, A. B., Peto, T. E., Crook, D. W., Butler, J. C., Kristinsson, K. G. & Spratt, B. G. ( 2004; ). Temporal and geographic stability of the serogroup-specific invasive disease potential of Streptococcus pneumoniae in children. J Infect Dis 190, 1203–1211.[CrossRef]
    [Google Scholar]
  4. Brueggemann, A. B., Pai, R., Crook, D. W. & Beall, B. ( 2007; ). Vaccine escape recombinants emerge after pneumococcal vaccination in the United States. PLoS Pathog 3, e168 [CrossRef]
    [Google Scholar]
  5. Center, K. J. ( 2007; ). PrevenarTM vaccination: review of the global data, 2006. Vaccine 25, 3085–3089.[CrossRef]
    [Google Scholar]
  6. CLSI ( 2009; ). Performance Standards for Antimicrobial Susceptibility Testing, 19th Informational Supplement. CLSI document M100-S19. Wayne, PA: Clinical and Laboratory Standards Institute.
  7. Enright, M. C. & Spratt, B. G. ( 1998; ). A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology 144, 3049–3060.[CrossRef]
    [Google Scholar]
  8. Feil, E. J., Li, B. C., Aanensen, D. M., Hanage, W. P. & Spratt, B. G. ( 2004; ). eburst: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 186, 1518–1530.[CrossRef]
    [Google Scholar]
  9. Hanage, W. P. ( 2008; ). Serotype-specific problems associated with pneumococcal conjugate vaccination. Future Microbiol 3, 23–30.[CrossRef]
    [Google Scholar]
  10. Hanage, W. P., Kaijalainen, T. H., Syrjänen, R. K., Auranen, K., Leinonen, M., Mäkelä, P. H. & Spratt, B. G. ( 2005; ). Invasiveness of serotypes and clones of Streptococcus pneumoniae among children in Finland. Infect Immun 73, 431–435.[CrossRef]
    [Google Scholar]
  11. Hausdorff, W. P., Bryant, J., Paradiso, P. R. & Siber, G. R. ( 2000; ). Which pneumococcal serogroups cause the most invasive disease: implications for conjugate vaccine formulation and use, part I. Clin Infect Dis 30, 100–121.[CrossRef]
    [Google Scholar]
  12. Hava, D. L., LeMieux, J. & Camilli, A. ( 2003; ). From nose to lung: the regulation behind Streptococcus pneumoniae virulence factors. Mol Microbiol 50, 1103–1110.[CrossRef]
    [Google Scholar]
  13. Marrie, T. J. ( 2000; ). Community-acquired pneumonia in the elderly. Clin Infect Dis 31, 1066–1078.[CrossRef]
    [Google Scholar]
  14. McGee, L., McDougal, L., Zhou, J., Spratt, B. G., Tenover, F. C., George, R., Hakenbeck, R., Hryniewicz, W., Lefévre, J. C. & other authors ( 2001; ). Nomenclature of major antimicrobial-resistant clones of Streptococcus pneumoniae defined by the pneumococcal molecular epidemiology network. J Clin Microbiol 39, 2565–2571.[CrossRef]
    [Google Scholar]
  15. Motlova, J., Benes, C. & Kriz, P. ( 2009; ). Incidence of invasive pneumococcal disease in the Czech Republic and serotype coverage by vaccines, 1997–2006. Epidemiol Infect 137, 562–569.[CrossRef]
    [Google Scholar]
  16. Sandgren, A., Sjostrom, K., Olsson-Liljequist, B., Christensson, B., Samuelsson, A., Kronvall, G. & Henriques Normark, B. ( 2004; ). Effect of clonal and serotype-specific properties on the invasive capacity of Streptococcus pneumoniae. J Infect Dis 189, 785–796.[CrossRef]
    [Google Scholar]
  17. Sorensen, U. B. ( 1993; ). Typing of pneumococci by using 12 pooled antisera. J Clin Microbiol 31, 2097–2100.
    [Google Scholar]
  18. Takala, A. K., Vuopio-Varkila, J., Tarkka, E., Leinonen, M. & Musser, J. M. ( 1996; ). Subtyping of common pediatric pneumococcal serotypes from invasive disease and pharyngeal carriage in Finland. J Infect Dis 173, 128–135.[CrossRef]
    [Google Scholar]
  19. Watson, D. A. & Musher, D. M. ( 1999; ). A brief history of the pneumococcus in biomedical research. Semin Respir Infect 14, 198–208.
    [Google Scholar]
  20. Zemlickova, H., Urbaskova, P., Adamkova, V., Motlova, J., Lebedova, V. & Prochazka, B. ( 2006; ). Characteristics of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus isolated from the nasopharynx of healthy children attending day-care centres in the Czech Republic. Epidemiol Infect 134, 1179–1187.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.018390-0
Loading
/content/journal/jmm/10.1099/jmm.0.018390-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 1079-1083

Results of e BURSTanalysis performed on the entire MLST datasets for serotype 6A and 23F isolates. [PDF](154 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error