1887

Abstract

O1 are classified into two biotypes, classical and El Tor, each encoding a biotype-specific cholera toxin. However, El Tor strains have recently emerged with a classical cholera-toxin genotype (El Tor variant). We characterized El Tor strains of O1 from travel-associated cases of cholera in Japan isolated from 1991 to 2006 by cholera toxin B subunit gene () typing and by molecular epidemiological methods. in the biotype El Tor shifted from the El Tor-specific type to the classical-specific type around 1993, and this type fully dominated the later half of the 1990s. Based on the results of PFGE and multilocus variable-number tandem repeat analysis, strains of the classical biotype remained diverse from those of El Tor biotype. The El Tor biotype strains formed multiple minor clusters and intermingled with each other irrespective of their origins and toxin types. El Tor variant strains are widespread in Asian countries and show significant genetic diversity, indicating that their spread is a result of multiclonal expansion rather than spread from a single clone.

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2010-06-01
2019-10-22
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References

  1. Bhattacharya, M. K., Bhattacharya, S. K., Garg, S., Saha, P. K., Dutta, D., Nair, G. B., Deb, B. C. & Das, K. P. ( 1993; ). Outbreak of Vibrio cholerae non-O1 in India and Bangladesh. Lancet 341, 1346–1347.
    [Google Scholar]
  2. Cooper, K. L., Luey, C. K., Bird, M., Terajima, J., Nair, G. B., Kam, K. M., Arakawa, E., Safa, A., Cheung, D. T. & other authors ( 2006; ). Development and validation of a PulseNet standardized pulsed-field gel electrophoresis protocol for subtyping of Vibrio cholerae. Foodborne Pathog Dis 3, 51–58.[CrossRef]
    [Google Scholar]
  3. Danin-Poleg, Y., Cohen, L. A., Grancz, H., Broza, Y. Y., Goldshmidt, H., Malul, E., Valinsky, L., Lerner, L., Broza, M. & Kashi, Y. ( 2007; ). Vibrio cholerae strain typing and phylogeny study based on simple sequence repeats. J Clin Microbiol 45, 736–746.[CrossRef]
    [Google Scholar]
  4. Faruque, S. M., Ahmed, K. M., Abdul Alim, A. R., Qadri, F., Siddique, A. K. & Albert, M. J. ( 1997; ). Emergence of a new clone of toxigenic Vibrio cholerae O1 biotype El Tor displacing V. cholerae O139 Bengal in Bangladesh. J Clin Microbiol 35, 624–630.
    [Google Scholar]
  5. Hyytiä-Trees, E., Smole, S. C., Fields, P. A., Swaminathan, B. & Ribot, E. M. ( 2006; ). Second generation subtyping: a proposed PulseNet protocol for multiple-locus variable-number tandem repeat analysis of Shiga toxin-producing Escherichia coli O157 (STEC O157). Foodborne Pathog Dis 3, 118–131.[CrossRef]
    [Google Scholar]
  6. Kaper, J. B., Morris, J. G., Jr & Levine, M. M. ( 1995; ). Cholera. Clin Microbiol Rev 8, 48–86.
    [Google Scholar]
  7. Morita, M., Ohnishi, M., Arakawa, E., Bhuiyan, N. A., Nusrin, S., Alam, M., Siddique, A. K., Qadri, F., Izumiya, H. & other authors ( 2008; ). Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor. Microbiol Immunol 52, 314–317.[CrossRef]
    [Google Scholar]
  8. Nair, G. B., Qadri, F., Holmgren, J., Svennerholm, A., Safa, A., Bhuiyan, N. A., Ahmad, Q. S., Faruque, S. M., Faruque, A. S. G. & other authors ( 2006; ). Cholera due to altered El Tor strains of Vibrio cholerae O1 in Bangladesh. J Clin Microbiol 44, 4211–4213.[CrossRef]
    [Google Scholar]
  9. Nguyen, B. M., Lee, J. H., Cuong, N. T., Choi, S. Y., Hien, N. T., Anh, D. D., Lee, H. R., Ansaruzzaman, M., Endtz, H. P. & other authors ( 2009; ). Cholera outbreaks caused by an altered El Tor Vibrio cholerae O1 El Tor biotype strain producing classical cholera toxin B in Vietnam in 2007 to 2008. J Clin Microbiol 47, 1568–1571.[CrossRef]
    [Google Scholar]
  10. Olsvik, Ø., Wahlberg, J., Petterson, B., Uhlén, M., Popovic, T., Wachsmuth, K. & Fields, P. I. ( 1993; ). Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains. J Clin Microbiol 31, 22–25.
    [Google Scholar]
  11. Popovic, T., Fields, P. I. & Olsvik, Ø. ( 1994; ). Detection of cholera toxin genes. In Vibrio Cholerae and Cholera: Molecular to Global Perspectives, pp. 41–52. Edited by I. K. Wachsmuth, P. A. Blake & Ø. Olsvik. Washington, DC: American Society for Microbiology.
  12. Raychoudhuri, A., Mukhopadhyay, A. K., Ramamurthy, T., Nandy, R. K., Takeda, Y. & Nair, G. B. ( 2008; ). Biotyping of Vibrio cholerae O1: time to redefine the scheme. Indian J Med Res 128, 695–698.
    [Google Scholar]
  13. Raychoudhuri, A., Patra, T., Ghosh, K., Ramamurthy, T., Nandy, R. K., Takeda, Y., Nair, G. B. & Mukhopadhyay, A. K. ( 2009; ). Classical ctxB in Vibrio cholerae O1, Kolkata, India. Emerg Infect Dis 15, 131–132.[CrossRef]
    [Google Scholar]
  14. Sack, D. A., Sack, R. B., Nair, G. B. & Siddique, A. K. ( 2004; ). Cholera. Lancet 363, 223–233.[CrossRef]
    [Google Scholar]
  15. Safa, A., Sultana, J., Cam, P. D., Mwansa, J. C. & Kong, R. Y. C. ( 2008; ). Vibrio cholerae O1 hybrid El Tor strains, Asia and Africa. Emerg Infect Dis 14, 987–988.[CrossRef]
    [Google Scholar]
  16. Udden, S. M. N., Zahid, M. S. H., Biswas, K., Ahmad, Q. S., Cravioto, A., Nair, G. B., Mekalanos, J. J. & Faruque, S. M. ( 2008; ). Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence. Proc Natl Acad Sci U S A 105, 11951–11956.[CrossRef]
    [Google Scholar]
  17. Van Heyningen, S. ( 1976; ). The subunits of cholera toxin: structure, stoichiometry and function. J Infect Dis 133 (Suppl.), 5–13.[CrossRef]
    [Google Scholar]
  18. Yamasaki, S., Nair, G. B., Bhattacharya, S. K., Yamamoto, S., Kurazono, H. & Takeda, Y. ( 1997; ). Cryptic appearance of a new clone of Vibrio cholerae serogroup O1 biotype El Tor in Calcutta, India. Microbiol Immunol 41, 1–6.[CrossRef]
    [Google Scholar]
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