1887

Abstract

The US Centers for Disease Control and Prevention lists as a potential bioterrorism threat requiring enhanced diagnostic capacity and surveillance (http://emergency.cdc.gov/bioterrorism/). Successful treatment and management of patients after exposure to biological threat agents depends on accurate and timely diagnosis, but many biothreat agents present with similar, vague clinical signs – commonly referred to as ‘flu-like illness’. Diagnosis of brucellosis is notoriously challenging, especially early in infection, and definitive diagnosis may require invasive methods, e.g. bone marrow biopsy. We studied the pathogenesis of aerosol infection in rhesus macaques in an effort to guide the diagnostic algorithm in case of possible intentional exposure of humans. Rhesus proved to be an excellent model for human brucellosis; the data showed that PCR DNA amplification testing of non-invasive diagnostic samples has the potential to definitively detect a point-source outbreak immediately and for several days after exposure.

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2010-06-01
2019-11-15
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References

  1. Akritidis, N., Tzivras, M., Delladetsima, I., Stefanaki, S., Moutsopoulos, H. M. & Pappas, G. ( 2007; ). The liver in brucellosis. Clin Gastroenterol Hepatol 5, 1109–1112.[CrossRef]
    [Google Scholar]
  2. Aleixo, M. J., Ferreira, M. L. & Antunes, F. ( 1999; ). Brucellosis. Acta Med Port 12, 323–330.
    [Google Scholar]
  3. Christensen, D. R., Hartman, L. J., Loveless, B. M., Frye, M. S., Shipley, M. A., Bridge, D. L., Richards, M. J., Kaplan, R. S., Garrison, J. & other authors ( 2006; ). Detection of biological threat agents by real-time PCR: comparison of assay performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler platforms. Clin Chem 52, 141–145.[CrossRef]
    [Google Scholar]
  4. Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council ( 1996; ). Guide for the Care and Use of Laboratory Animals. National Institutes of Health Publication No. 86–23. Washington, DC: National Institutes of Health.
  5. Dabisch, P. A., Kline, J. L., Lewis, C., Yeager, J. J. & Pitt, M. L. M. ( 2010; ). Characterization of a head-only aerosol exposure chamber for nonhuman primates. Inhal Toxicol 22, 224–233.[CrossRef]
    [Google Scholar]
  6. Demirtürk, N., Demirdal, T., Erben, N., Demir, S., Asci, Z., Kilit, T. P. & Kartal, E. D. ( 2008; ). Brucellosis: a retrospective evaluation of 99 cases and review of brucellosis treatment. Trop Doct 38, 59–62.[CrossRef]
    [Google Scholar]
  7. Franco, M. P., Mulder, M., Gilman, R. H. & Smits, H. L. ( 2007; ). Human brucellosis. Lancet Infect Dis 7, 775–786.[CrossRef]
    [Google Scholar]
  8. Franz, D. R., Jahrling, P. B., McClain, D. J., Hoover, D. L., Byrne, W. R., Pavlin, J. A., Christopher, G. W., Cielak, T. J., Friedlander, A. M. & Eitzen, E. M., Jr ( 2001; ). Clinical recognition and management of patients exposed to biological warfare agents. Clin Lab Med 21, 435–473.
    [Google Scholar]
  9. Hoover, D. L. & Friedlander, A. M. ( 1997; ). Brucellosis. In Textbook of Military Medicine: Medical Aspects of Chemical and Biological Warfare, pp. 513–521. Edited by F. R. Sidell and others. Washington, DC: Borden Institute.
  10. Kumar, V., Abbas, K., Fausto, N. (editors) ( 2005; ). Robbins and Cotran Pathologic Basis of Disease, 7th edn, pp. 50–51. Philadelphia, PA: Elsevier Saunders.
  11. Maquart, M., Fardini, Y., Zygmunt, M. S. & Cloeckaert, A. ( 2008; ). Identification of novel DNA fragments and partial sequence of a genomic island specific of Brucella pinnipedialis. Vet Microbiol 132, 181–189.[CrossRef]
    [Google Scholar]
  12. Mense, M. G., Borschel, R. H., Wilhelmsen, C. L., Pitt, M. L. & Hoover, D. L. ( 2004; ). Pathologic changes associated with brucellosis experimentally induced by aerosol exposure in rhesus macaques (Macaca mulatta). Am J Vet Res 65, 644–652.[CrossRef]
    [Google Scholar]
  13. Theegarten, D., Albrecht, S., Tötsch, M., Teschler, H., Neubauer, H. & Al Dahouk, S. ( 2008; ). Brucellosis of the lung: case report and review of the literature. Virchows Arch 452, 97–101.[CrossRef]
    [Google Scholar]
  14. Whatmore, A. M., Dawson, C. E., Groussaud, P., Koylass, M. S., King, A. C., Shankster, S. J., Sohn, A. H., Probert, W. S. & McDonald, W. L. ( 2008; ). Marine mammal Brucella genotype associated with zoonotic infection. Emerg Infect Dis 14, 517–518.[CrossRef]
    [Google Scholar]
  15. Yagupsky, P., Peled, N., Press, J., Abu-Rashid, M. & Abramson, O. ( 1997; ). Rapid detection of Brucella melitensis from blood cultures by a commercial system. Eur J Clin Microbiol Infect Dis 16, 605–607.[CrossRef]
    [Google Scholar]
  16. Young, E. J. ( 1983; ). Human brucellosis. Rev Infect Dis 5, 821–842.[CrossRef]
    [Google Scholar]
  17. Young, E. J. ( 1995; ). An overview of human brucellosis. Clin Infect Dis 21, 283–289.[CrossRef]
    [Google Scholar]
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