1887

Abstract

A typing system for using sequencing of the surface-layer protein A encoding gene () was evaluated and used to analyse clinical isolates in Japan. A total of 160 stool specimens from symptomatic patients in Japan was examined and 87 isolates were recovered. sequence typing was found to have reliable typability and discriminatory power in comparison with PCR ribotyping, and the typing results were highly reproducible and comparable. sequence typing was used to type in DNA extracted directly from stool specimens. Among the 90 stool specimens in which direct typing results were obtained, 77 specimens were positive for culture, and typing results from isolated strains agreed with those from direct typing in all 77 specimens. The sequence type smz was dominant at all four hospitals examined, and this endemic type was detected by culture and/or direct typing in 61 (62 %) of 99 stool specimens positive for toxic culture and/or direct sequence typing. Comparison of epidemic strains reported throughout the world revealed one isolate identified as sequence type gc8, which was found to correspond to PCR ribotype 027 (BI/NAP1/027), whereas no isolates were found with the gene identical to that of PCR ribotype 078 strain. sequence typing is valuable for comparison of strains epidemic in diverse areas because the typing results are reproducible and can easily be shared. In addition, sequence typing could be applied to direct typing without culture.

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2010-05-01
2019-11-18
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