1887

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Volume 59, Issue 6

Molecular epidemiology of vancomycin-resistant strains isolated from haematological malignancy patients in a research hospital in Turkey Free

Abstract

Infections and outbreaks of vancomycin-resistant enterococci (VRE) still appear to be rare in Turkey. In the present study, VRE strains isolated during an outbreak in a haematology unit of a training and research hospital in Turkey were typed and their antimicrobial-resistance patterns were characterized by molecular methods. Twelve vancomycin-resistant strains isolated from patients with haematological malignancies were investigated by PCR for the presence of genes encoding resistance to vancomycin, tetracycline, chloramphenicol, gentamicin and erythromycin. Their clonal relationship was evaluated by PFGE and multilocus sequence typing. All strains were resistant to vancomycin and erythromycin, and had the and genes, respectively. PFGE was used to determine the presence of two pulsotypes and determine their subtypes. Pulsotype A belonged to sequence type (ST) 17 and pulsotype B belonged to ST 78. All strains with the gene were not the same clone, indicating multiple acquisitions of resistant isolates, even over such a short time period.

  • Received:
  • Accepted:
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Keyword(s): CC, clonal complex , MLST, multilocus sequence typing , NEQAS, National External Quality Assessment Scheme for Microbiology , ST, sequence type and VRE, vancomycin-resistant enterococci
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2010-06-01
2024-04-27
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Molecular epidemiology of vancomycin-resistant Enterococcus faecium strains isolated from haematological malignancy patients in a research hospital in Turkey
J. Med. Microbiol. 59, 660 (2010); https://doi.org/10.1099/jmm.0.012625-0
/content/journal/jmm/10.1099/jmm.0.012625-0
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