1887

Abstract

The genus includes many bacteria that cause serious human infections. As is the case with other Gram-negative bacteria, species produce LPS, which is an abundant component of the bacterial cell surface. complex (Bcc) bacteria (which include at least 17 separate species) produce LPS structures that are quite different. In an attempt to determine the degree of LPS epitope variation among Bcc species, a mAb was produced, designated 5D8, specific for the LPS of . Western blot analysis determined that mAb 5D8 was able to produce the classic ‘ladder pattern’ when used to probe and lysates, although 5D8 did not produce this pattern with the other seven Bcc species tested. mAb 5D8 reacted with varying intensity to most but not all of the additional and strains tested. Therefore, there seems to be significant epitope variation among Bcc LPS both between and within species. Additionally, mAb 5D8 reacted with a proteinase-K-sensitive 22 kDa antigen in all Bcc strains and also in a strain of .

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2010-01-01
2019-10-18
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References

  1. Brandt, S., Thorkildson, P. & Kozel, T. R. ( 2003; ). Monoclonal antibodies reactive with immunorecessive epitopes of glucuronoxylomannan, the major capsular polysaccharide of Cryptococcus neoformans. Clin Diagn Lab Immunol 10, 903–909.
    [Google Scholar]
  2. Brett, P. J., DeShazer, D. & Woods, D. E. ( 1998; ). Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species. Int J Syst Bacteriol 48, 317–320.[CrossRef]
    [Google Scholar]
  3. Burtnick, M. N., Brett, P. J. & Woods, D. E. ( 2002; ). Molecular and physical characterization of Burkholderia mallei O antigens. J Bacteriol 184, 849–852.[CrossRef]
    [Google Scholar]
  4. Carillo, S., Silipo, A., Perino, V., Lanzetta, R., Parrilli, M. & Molinaro, A. ( 2009; ). The structure of the O-specific polysaccharide from the lipopolysaccharide of Burkholderia anthina. Carbohydr Res 344, 1697–1700.[CrossRef]
    [Google Scholar]
  5. Cerantola, S., Lemassu-Jacquier, A. & Montrozier, H. ( 1999; ). Structural elucidation of a novel exopolysaccharide produced by a mucoid clinical isolate of Burkholderia cepacia. Characterization of a trisubstituted glucuronic acid residue in a heptasaccharide repeating unit. Eur J Biochem 260, 373–383.
    [Google Scholar]
  6. Cescutti, P., Impallomeni, G., Garozzo, D., Sturiale, L., Herasimenka, Y., Lagatolla, C. & Rizzo, R. ( 2003; ). Exopolysaccharides produced by a clinical strain of Burkholderia cepacia isolated from a cystic fibrosis patient. Carbohydr Res 338, 2687–2695.[CrossRef]
    [Google Scholar]
  7. Chu, K. K., Davidson, D. J., Halsey, T. K., Chung, J. W. & Speert, D. P. ( 2002; ). Differential persistence among genomovars of the Burkholderia cepacia complex in a murine model of pulmonary infection. Infect Immun 70, 2715–2720.[CrossRef]
    [Google Scholar]
  8. Coenye, T., Vandamme, P., LiPuma, J. J., Govan, J. R. & Mahenthiralingam, E. ( 2003; ). Updated version of the Burkholderia cepacia complex experimental strain panel. J Clin Microbiol 41, 2797–2798.[CrossRef]
    [Google Scholar]
  9. Conway, B. A., Chu, K. K., Bylund, J., Altman, E. & Speert, D. P. ( 2004; ). Production of exopolysaccharide by Burkholderia cenocepacia results in altered cell-surface interactions and altered bacterial clearance in mice. J Infect Dis 190, 957–966.[CrossRef]
    [Google Scholar]
  10. Cox, A. D. & Wilkinson, S. G. ( 1990; ). Structure of the O-specific polymer for Pseudomonas cepacia serogroup O7. Carbohydr Res 198, 153–156.[CrossRef]
    [Google Scholar]
  11. De Soyza, A., Silipo, A., Lanzetta, R., Govan, J. R. & Molinaro, A. ( 2008; ). Chemical and biological features of Burkholderia cepacia complex lipopolysaccharides. Innate Immun 14, 127–144.[CrossRef]
    [Google Scholar]
  12. DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. ( 1956; ). Colorimetric method for determination of sugars and related substances. Anal Chem 28, 350–356.[CrossRef]
    [Google Scholar]
  13. Evans, E., Poxton, I. R. & Govan, J. R. ( 1999; ). Lipopolysaccharide chemotypes of Burkholderia cepacia. J Med Microbiol 48, 825–832.[CrossRef]
    [Google Scholar]
  14. Faure, R., Shiao, T. C., Lagnoux, D., Giguere, D. & Roy, R. ( 2007; ). En route to a carbohydrate-based vaccine against Burkholderia cepacia. Org Biomol Chem 5, 2704–2708.[CrossRef]
    [Google Scholar]
  15. Fomsgaard, A., Freudenberg, M. A. & Galanos, C. ( 1990; ). Modification of the silver staining technique to detect lipopolysaccharide in polyacrylamide gels. J Clin Microbiol 28, 2627–2631.
    [Google Scholar]
  16. Jones, S. M., Ellis, J. F., Russell, P., Griffin, K. F. & Oyston, P. C. ( 2002; ). Passive protection against Burkholderia pseudomallei infection in mice by monoclonal antibodies against capsular polysaccharide, lipopolysaccharide or proteins. J Med Microbiol 51, 1055–1062.
    [Google Scholar]
  17. Kenna, D. T., Barcus, V. A., Langley, R. J., Vandamme, P. & Govan, J. R. ( 2003; ). Lack of correlation between O-serotype, bacteriophage susceptibility and genomovar status in the Burkholderia cepacia complex. FEMS Immunol Med Microbiol 35, 87–92.[CrossRef]
    [Google Scholar]
  18. Knirel' Iu, A., Shashkov, A. S., Soldatkina, M. A., Paramonov, N. A. & Zakharova, I. ( 1988a; ). Antigenic polysaccharides of bacteria. 32. The structure of O-specific polysaccharide chains of Pseudomonas cepacia serotype B and E lipopolysaccharides containing d-fucose. Bioorg Khim 14, 1208–1213. (in Russian)
    [Google Scholar]
  19. Knirel' Iu, A., Soldatkina, M. A., Shashkov, A. S., Tanamar, N. V. & Paramonov, N. A. ( 1988b; ). Antigenic polysaccharides of bacteria. 34. Structure of O-specific polysaccharide chains of lipopolysaccharides from Pseudomonas cepacia strains IMV 4207 (Serotype A) and IMV 598/2. Bioorg Khim 14, 1678–1683. (in Russian)
    [Google Scholar]
  20. Kozel, T. R., Murphy, W. J., Brandt, S., Blazar, B. R., Lovchik, J. A., Thorkildson, P., Percival, A. & Lyons, C. R. ( 2004; ). mAbs to Bacillus anthracis capsular antigen for immunoprotection in anthrax and detection of antigenemia. Proc Natl Acad Sci U S A 101, 5042–5047.[CrossRef]
    [Google Scholar]
  21. Lagatolla, C., Skerlavaj, S., Dolzani, L., Tonin, E. A., Monti Bragadin, C., Bosco, M., Rizzo, R., Giglio, L. & Cescutti, P. ( 2002; ). Microbiological characterisation of Burkholderia cepacia isolates from cystic fibrosis patients: investigation of the exopolysaccharides produced. FEMS Microbiol Lett 209, 99–106.[CrossRef]
    [Google Scholar]
  22. Leone, S., Molinaro, A., Gerber, I. B., Dubery, I. A., Lanzetta, R. & Parrilli, M. ( 2006; ). The O-chain structure from the LPS of the endophytic bacterium Burkholderia cepacia strain ASP B 2D. Carbohydr Res 341, 2954–2958.[CrossRef]
    [Google Scholar]
  23. LiPuma, J. J. ( 2005; ). Update on the Burkholderia cepacia complex. Curr Opin Pulm Med 11, 528–533.[CrossRef]
    [Google Scholar]
  24. Mahenthiralingam, E., Coenye, T., Chung, J. W., Speert, D. P., Govan, J. R., Taylor, P. & Vandamme, P. ( 2000; ). Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex. J Clin Microbiol 38, 910–913.
    [Google Scholar]
  25. Mahenthiralingam, E., Urban, T. A. & Goldberg, J. B. ( 2005; ). The multifarious, multireplicon Burkholderia cepacia complex. Nat Rev Microbiol 3, 144–156.[CrossRef]
    [Google Scholar]
  26. Nakamura, Y., Hyodo, S., Chonan, E., Shigeta, S. & Yabuuchi, E. ( 1986; ). Serological classification of Pseudomonas cepacia by somatic antigen. J Clin Microbiol 24, 152–154.
    [Google Scholar]
  27. Rabkin, C. S., Jarvis, W. R., Anderson, R. L., Govan, J., Klinger, J., LiPuma, J., Martone, W. J., Monteil, H., Richard, C. & other authors ( 1989; ). Pseudomonas cepacia typing systems: collaborative study to assess their potential in epidemiologic investigations. Rev Infect Dis 11, 600–607.[CrossRef]
    [Google Scholar]
  28. Shaw, D., Poxton, I. R. & Govan, J. R. ( 1995; ). Biological activity of Burkholderia (Pseudomonas) cepacia lipopolysaccharide. FEMS Immunol Med Microbiol 11, 99–106.[CrossRef]
    [Google Scholar]
  29. Soldatkina, A., Knirel' Iu, A., Tanatar, N. V. & Zakharova, I. ( 1989; ). Immunologic and structural studies of lipopolysaccharides from Pseudomonas cepacia. Mikrobiol Zh 51, 32–38.
    [Google Scholar]
  30. Steinmetz, I., Rohde, M. & Brenneke, B. ( 1995; ). Purification and characterization of an exopolysaccharide of Burkholderia (Pseudomonas) pseudomallei. Infect Immun 63, 3959–3965.
    [Google Scholar]
  31. Tomlin, K. L., Coll, O. P. & Ceri, H. ( 2001; ). Interspecies biofilms of Pseudomonas aeruginosa and Burkholderia cepacia. Can J Microbiol 47, 949–954.[CrossRef]
    [Google Scholar]
  32. Tsai, C. M. & Frasch, C. E. ( 1982; ). A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119, 115–119.[CrossRef]
    [Google Scholar]
  33. Vinion-Dubiel, A. D. & Goldberg, J. B. ( 2003; ). Lipopolysaccharide of Burkholderia cepacia complex. J Endotoxin Res 9, 201–213.
    [Google Scholar]
  34. Vinion-Dubiel, A. D., Spilker, T., Dean, C. R., Monteil, H., LiPuma, J. J. & Goldberg, J. B. ( 2004; ). Correlation of wbiI genotype, serotype, and isolate source within species of the Burkholderia cepacia complex. J Clin Microbiol 42, 4121–4126.[CrossRef]
    [Google Scholar]
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