1887

Abstract

Thirteen patients with leptospirosis were identified, as confirmed by laboratory analysis during the last 5 years in our laboratory, who came from urban areas of Tokyo, Japan. All of the patients came into contact with rats before the onset of illness. Seventeen per cent of Norway rats captured in the inner cities of Tokyo carried leptospires in their kidneys. Most of these rat isolates were serovar Copenhageni/Icterohaemorrhagiae. Antibodies against these serovars and their DNA were detected in the patients. This suggests that rats are important reservoirs of leptospirosis, and that rat-borne leptospires occur in urban areas of Tokyo.

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2009-09-01
2019-11-13
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Results of MAT, IgM detection and PCR. [ PDF file] (27 KB)

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PFGE of I restriction fragments of isolates of the rats and the reference strains of serovars Copenhageni (M20) and Icterohaemorrhagiae (RGA and Ictero no. 1). Lanes: 1, F-1; 2, F-3; 3, F-4; 4, F-6; 5, F-10; 6, F-16; 7, F-19; 8, F-20; 9, F-22; 10, F-24; 11, F-25; 12, F-28; 13 and 23, M20; 14 and 22, PGA; 15 and 24, Ictero no. 1; 16, H-2; 17, H-3; 18, H-4; 19, G-1; 20, K-6; 21, M-1; M, molecular mass marker (λ ladder; Bio-Rad Laboratories). Alphabets of each isolates correlate with those of each locations in Table 1. [ PDF file] (409 KB)

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