1887

Abstract

is the species most frequently isolated from oral specimens, but the recovery of other species such as is increasing. Differentiation of from requires special tests and both species are misidentified in some studies. CHROM-Pal (CH-P) is a novel chromogenic medium used in our laboratory for differentiation between and on the basis of colony colour and morphology, and chlamydospore production. The performance of CH-P and CHROMagar Candida (CAC) was compared for primary isolation and presumptive identification of yeasts from oral specimens from human immunodeficiency virus (HIV)-infected and uninfected individuals. The identification of species on both media was compared with two reference identification methods (API ID 32 C and multiplex PCR). A total of 137/205 oral swabs (66.8 %) plated onto CH-P and CAC media were positive by culture and resulted in the growth of 171 isolates of species on CH-P, whilst only 159 isolates grew on CAC. was the most frequently isolated species in both groups of patients, followed by in the HIV-negative group, and by in the HIV-infected group. The other species isolated were , , , , , , , and . The sensitivity and specificity for identifying , , and on CH-P were over 98.5 %, always equal to or higher than those obtained when CAC was used. CH-P is a simple reliable medium for primary isolation and presumptive identification of yeast isolates from oral samples. The ability of CH-P to discriminate between and was significantly higher ( <0.05) than that of CAC.

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2009-11-01
2019-10-22
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