1887

Abstract

is a tick-borne rickettsial pathogen that has measurable genetic heterogeneity across its geographical range and reservoir spectrum. In the present study, publicly available sequences of the genes that have prevailingly been used for typing were analysed to identify the segments giving the highest resolution with respect to the predictability of host and geographical provenances of the strains. Selected partial sequences of 16S rRNA, L, 4 and genes were then employed in a tentative multilocus typing scheme used to characterize the strains causing equine granulocytic anaplasmosis (EGA). We were able to both identify alleles characteristic for equine strains of and distinguish two unique genetic variants infecting horses in the Czech Republic. This resolution far exceeded the discriminatory potential of any of the four sequenced genes when used singly. The two novel variants appeared to be phylogenetically closer to the strains reported as causing human disease in Slovenia than to strains thus far isolated from other European EGA cases. A decline in the quality of recently deposited sequences was also demonstrated.

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2009-04-01
2019-10-13
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Residue polymorphism analysis of 78 SL sequences: (a, b) SL nucleotide sequences; (c, d) translated GroL amino acid sequences (for further details see Fig. 2). [ PDF file] (137 KB)

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Residue polymorphism analysis of 43 4 sequences: (a, b) 4 nucleotide sequences; (c, d) translated MSP-4 amino acid sequences; (d) predicted inner, trans-membrane and outer portions of the protein are shown below the baseline (for further details see Fig. 2). [ PDF file] (77 KB)

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Residue polymorphism analysis of 35 sequences: (a, b) nucleotide sequences; (c, d) translated Ank amino acid sequences (for further details see Fig. 2). [ PDF file] (89 KB)

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