1887

Abstract

is a known pathogen of dental caries and its major cell surface antigens have been widely investigated. Recently, an approximately 120 kDa Cnm protein with binding properties to type I collagen was identified, and its encoding gene () cloned and sequenced. In the present study, we sequenced from 47 different clinical strains and found that the nucleotide alignment of the collagen-binding domain was well conserved. We devised a PCR method for identifying the gene, examined the prevalence of -positive strains in various mother–child groups, and assessed the significance of such strains for transmission and dental caries. The detection rate of -positive strains was significantly lower in strains isolated from Japanese children in the 2000s (8.0 %) as compared to those isolated in the 1980s (15.8 %) (<0.05). Furthermore, the presence of possessing in salivary specimens collected from 55 -positive mother–child pairs was 40 and 32.7 % in the mothers and children, respectively. The frequency of -positive children whose mothers were also positive was 72 %, which was significantly higher than that of -positive children with negative mothers (<0.0001, odds ratio 17.5). In addition, clinical parameters indicating dental caries were significantly increased in children with -positive in saliva (=13), as compared to those with -negative (=15) and -negative children (=20) (<0.01). These results indicate that -positive strains are closely correlated with dental caries, while vertical transmission in -positive mother–child pairs was also demonstrated.

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2009-04-01
2019-12-15
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