1887

Journal of Medical Microbiology logo

Volume 58, Issue 5

Host Wnt/-catenin pathway triggered by correlates with regression of gastric intestinal metaplasia after eradication Free

Abstract

eradication can reverse gastric intestinal metaplasia (IM) in some but not all patients. induces high levels of nuclear -catenin staining in IM tissues, as well as overexpression of cyclooxygenase-2 (COX-2). This study investigated whether the Wnt/-catenin pathway plays a role in IM regression following eradication. Sixty-five -infected patients with IM who had achieved successful eradication provided paired gastric samples before and after eradication to analyse the persistence of IM, and to assess COX-2 and nuclear -catenin expression. The host genotypes of single nucleotide polymorphisms (SNPs) of the , -catenin () and adenomatous polyposis coli () genes were analysed. In addition, expression of -catenin, E-cadherin and phosphorylated and unphosphorylated glycogen synthase kinase 3 (GSK-3) in cell lines challenged with isolates from patients with and without IM persistence was compared by immunoanalysis. After a mean 33.9-month follow-up after eradication, 44 patients (67.7 %) with IM persistence had a higher rate of high-level nuclear -catenin expression in IM tissue than those without IM persistence (=0.008). The patients with IM persistence had a higher rate of AA, GG and AA SNP genotypes at positions 4479, 5268 and 5465, respectively, than the patients without IM persistence (=0.022). The isolates from the patients with IM regression after eradication induced more phospho-GSK-3 in AGS cells than isolates from patients with IM persistence (=0.011). It is likely that interactions with and the patient's Wnt/-catenin genetic predisposition determine the outcome of IM persistence following eradication.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CI, confidence interval , COX-2, cyclooxygenase-2 , GSK-3β, glycogen synthase kinase 3β , IM, intestinal metaplasia and SNP, single nucleotide polymorphism
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2009-05-01
2024-04-26
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Host Wnt/β-catenin pathway triggered by Helicobacter pylori correlates with regression of gastric intestinal metaplasia after H. pylori eradication
J. Med. Microbiol. 58, 567 (2009); https://doi.org/10.1099/jmm.0.007310-0
/content/journal/jmm/10.1099/jmm.0.007310-0
/content/journal/jmm/10.1099/jmm.0.007310-0
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