1887

Abstract

The serotyping of O and H antigens is an important first step in the characterization of . However, serotyping has become increasingly technically demanding and expensive to perform. We have therefore sequenced additional O antigen gene clusters to provide information for the development of DNA-based serotyping methods. Three isolates had O antigen gene clusters with homology to the O123 O antigen region. O antigen clusters from two serogroup O58 strains had approximately 85 % identity with the O123 O antigen region over their entire length, suggesting that these and O antigen regions evolved from a common ancestor. The O antigen cluster of a serogroup O41 isolate had a lower level of identity with O123 over only part of its O antigen DNA cluster sequence, suggesting a different and more complex evolution of this gene cluster than those in the O58 strains. A large part of the O41 O antigen DNA cluster had very close identity with the O antigen cluster of an O62 strain. This region of DNA homology included the and genes. Therefore, molecular serotyping tests using only the O41 or O62 and genes would not differentiate between the two serogroups. The O123 O-antigenic polysaccharide and its repeating unit were characterized, and the chemical structure for O123 was entirely consistent with the O antigen gene cluster sequences of O123 and the O58 isolates. An understanding of both the genetic and structural composition of and O antigens is necessary for the development of novel molecular methods for serotyping these organisms.

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2009-07-01
2024-12-10
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References

  1. Ballmer K., Korczak B. M., Kuhnert P., Slickers P., Ehricht R., Hächler H. 2007; Fast DNA serotyping of Escherichia coli by use of an oligonucleotide microarray. J Clin Microbiol 45:370–379 [CrossRef]
    [Google Scholar]
  2. Baumann R., Wider G., Ernst R. R., Wüthrich K. 1981; Improvement of 2D NOE and 2D correlated spectra by symmetrization. J Magn Reson 44:402–406
    [Google Scholar]
  3. Bax A., Davis D. G. 1985; MLEV-17-based two-dimensional homonuclear magnetization transfer spectroscopy. J Magn Reson 65:355–360
    [Google Scholar]
  4. Bax A., Freeman R. 1981; Investigation of complex networks of spin-spin coupling by two-dimensional NMR. J Magn Reson 44:542–561
    [Google Scholar]
  5. Bax A., Subramanian S. 1986; Sensitivity-enhanced two-dimensional heteronuclear shift correlation NMR spectroscopy. J Magn Reson 67:565–569
    [Google Scholar]
  6. Bax A., Summers M. F. 1986; 1H and 13C assignments from sensitivity-enhanced detection of heteronuclear multiple-bond connectivity by 2D multiple quantum NMR. J Am Chem Soc 108:2093–2094 [CrossRef]
    [Google Scholar]
  7. Beutin L., Wang Q., Naumann D., Han W., Krause G., Leomil L., Wang L., Feng L. 2007; Relationship between O-antigen subtypes, bacterial surface structures and O-antigen gene clusters in Escherichia coli O123 strains carrying genes for Shiga toxin and intimin. J Med Microbiol 56:177–184 [CrossRef]
    [Google Scholar]
  8. Bock K., Thøgersen H. 1982; Nuclear magnetic resonance spectroscopy in the study of mono- and oligosaccharides. Annu Rep NMR Spectrosc 13:1–57
    [Google Scholar]
  9. Boyd E. F., Wang F. S., Whittam T. S., Selander R. K. 1996; Molecular genetic relationships of the salmonellae. Appl Environ Microbiol 62:804–808
    [Google Scholar]
  10. Dmitriev B. A., Backinovsky L. V., Kochetkov N. K., Khomenko N. A. 1973; Immunochemical studies on Shigella boydii lipopolysaccharides. Eur J Biochem 34:513–518 [CrossRef]
    [Google Scholar]
  11. Dmitriev B. A., Lvov V., Tochtamysheva N. V., Shashkov A. S., Kochetkov N. K., Jann B., Jann K. 1983; Cell-wall lipopolysaccharide of Escherichia coli 0114 : H2. Structure of the polysaccharide chain. Eur J Biochem 134:517–521 [CrossRef]
    [Google Scholar]
  12. Ewing W. H. 1986 Edwards & Ewing's Identification of Enterobacteriaceae , 4th edn. New York: Elsevier;
    [Google Scholar]
  13. Fitzgerald C., Sherwood R., Gheesling L., Brenner F. W., Fields P. I. 2003; Molecular analysis of the rfb O antigen gene cluster of Salmonella enterica serogroup O : 6,14 and development of a serogroup-specific PCR assay. Appl Environ Microbiol 69:6099–6105 [CrossRef]
    [Google Scholar]
  14. Fitzgerald C., Collins M., van Duyne S., Mikoleit M., Brown T., Fields P. 2007; Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups. J Clin Microbiol 45:3323–3334 [CrossRef]
    [Google Scholar]
  15. Grimont P. A. D., Weill F.-X. 2007 Antigenic Formulae of the Salmonella Serovars , 9th edn. Paris: Institut Pasteur;
    [Google Scholar]
  16. Hobbs M., Reeves P. R. 1994; The JUMPstart sequence: a 39 bp element common to several polysaccharide gene clusters. Mol Microbiol 12:855–856 [CrossRef]
    [Google Scholar]
  17. Kasper D. L., Weintraub A., Lindberg A. A., Lönngren J. 1983; Capsular polysaccharides and lipopolysaccharides from two Bacteroides fragilis reference strains: chemical and immunochemical characterization. J Bacteriol 153:991–997
    [Google Scholar]
  18. Kjær M., Andersen K. V., Poulsen F. M. 1994; Automated and semiautomated analysis of heteronuclear and multidimensional nuclear magnetic resonance spectra of proteins: the program PRONTO. Methods Enzymol 239:288–308
    [Google Scholar]
  19. Knirel Y. A., Vinogradov E. V., Shashkov A. S., Dmitriev B. A., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. 1986; Somatic antigens of Pseudomonas aeruginosa . The structure of O-specific polysaccharide chains of P. aeruginosa O10 (Lányi) lipopolysaccharide. Eur J Biochem 157:129–138 [CrossRef]
    [Google Scholar]
  20. Knirel Y. A., Dashunin V. V., Shashkov A. S., Kochetkov N. K., Dmitriev B. A., Hofman L. L. 1988; Somatic antigens of Shigella : structure of the O-specific polysaccharide chain of the Shigella dysentery type 7 lipopolysaccharide. Carbohydr Res 179:51–60 [CrossRef]
    [Google Scholar]
  21. Knirel Y. A., Paramonov N. A., Shashkov A. S., Kochetkov N. K., Zdorovenko G. M., Veremeychenko S. N., Zakharova I. Y. 1993; Somatic antigens of pseudomonads: structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar A strain IMV 1152. Carbohydr Res 243:205–210 [CrossRef]
    [Google Scholar]
  22. Kochetkov N. K., Chizhov O. S., Shashkov A. S. 1984; Dependence of 13C chemical shifts on the spatial interaction of protons, and its application in structural and conformational studies of oligo- and poly-saccharides. Carbohydr Res 133:173–185 [CrossRef]
    [Google Scholar]
  23. LeMinor L. 1997 Guidelines for the Preparation of Salmonella antisera Paris: World Health Organization Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur;
    [Google Scholar]
  24. LeMinor L., Popoff M. Y. 1997 Antigenic Formulas of the Salmonella Serovars , 7th edn. Paris: World Health Organization Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur;
    [Google Scholar]
  25. Leontein K., Lindberg B., Lönngren J. 1978; Assignment of absolute configuration of sugars by g.l.c. of their acetylated glycosides formed from chiral alcohols. Carbohydr Res 62:359–362 [CrossRef]
    [Google Scholar]
  26. Li Q., Reeves P. R. 2000; Genetic variation of the dTDP-l-rhamnose pathway genes in Salmonella enterica . Microbiology 146:2291–2307
    [Google Scholar]
  27. Lindberg A. A., Le Minor L. 1984; Serology of Salmonella . Methods Microbiol 15:1–141
    [Google Scholar]
  28. Linnerborg M., Wollin R., Widmalm G. 1997; Structural studies of the O-antigenic polysaccharide from Escherichia coli O167. Eur J Biochem 246:565–573 [CrossRef]
    [Google Scholar]
  29. Lipkind G. M., Shashkov A. S., Knirel Y. A., Vinogradov E. V., Kochetkov N. K. 1988; A computer-assisted structural analysis of regular polysaccharides on the basis of 13C-NMR data. Carbohydr Res 175:59–75 [CrossRef]
    [Google Scholar]
  30. Ørskov F., Ørskov I. 1984; Serotyping of Escherichia coli . Methods Microbiol 14:44–112
    [Google Scholar]
  31. Ørskov I., Ørskov F., Jann B., Jann K. 1977; Serology, chemistry, and genetics of O and K antigens of Escherichia coli . Bacteriol Rev 41:667–710
    [Google Scholar]
  32. Phillips L. R., Fraser B. A. 1981; Methylation of carbohydrates with dimsyl potassium in dimethyl sulfoxide. Carbohydr Res 90:149–152 [CrossRef]
    [Google Scholar]
  33. Porwollik S., Boyd E. F., Choy C., Cheng P., Florea L., Proctor E., McClelland M. 2004; Characterization of Salmonella enterica subspecies I genovars by use of microarrays. J Bacteriol 186:5883–5898 [CrossRef]
    [Google Scholar]
  34. Ribot E. M., Fair M. A., Gautom R., Cameron D. N., Hunter S. B., Swaminathan B., Barrett T. J. 2006; Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157 : H7, Salmonella , and Shigella for PulseNet. Foodborne Pathog Dis 3:59–67 [CrossRef]
    [Google Scholar]
  35. Sambrook J., Russell D. W. 2001 Molecular Cloning , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  36. Samuel G., Reeves P. 2003; Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly. Carbohydr Res 338:2503–2519 [CrossRef]
    [Google Scholar]
  37. Sharp P. M. 1991; Determinants of DNA sequence divergence between Escherichia coli and Salmonella typhimurium : codon usage, map position, and concerted evolution. J Mol Evol 33:23–33 [CrossRef]
    [Google Scholar]
  38. Shashkov A. S., Arbatsky N. P., Widmalm G., Knirel Y. A., Zych K., Sidorczyk Z. 1998; Structure and serological specificity of the O-specific polysaccharide of Proteus penneri strain 26. Eur J Biochem 253:730–733 [CrossRef]
    [Google Scholar]
  39. Threlfall E. J., Frost J. A. 1990; The identification, typing and fingerprinting of Salmonella : laboratory aspects and epidemiological applications. J Appl Bacteriol 68:5–16 [CrossRef]
    [Google Scholar]
  40. Vinogradov E. V., Kaca W., Rozalski A., Shashkov A. S., Cedzynski M., Knirel Y. A., Kochetkov N. K. 1991; Structural and immunochemical studies of O-specific polysaccharide of Proteus vulgaris 5/43 belonging to OX19 group (O-variants). Eur J Biochem 200:195–201 [CrossRef]
    [Google Scholar]
  41. Vinogradov E. V., Knirel Y. A., Kochetkov N. K., Schlecht S., Mayer H. 1994; The structure of the O-specific polysaccharide of Salmonella arizonae O62. Carbohydr Res 253:101–110 [CrossRef]
    [Google Scholar]
  42. Wang W., Perepelov A. V., Feng L., Shevelev S. D., Wang Q., Senchenkova S. N., Han W., Li Y., Shashkov A. S. other authors 2007; A group of Escherichia coli and Salmonella enterica O antigens sharing a common backbone structure. Microbiology 153:2159–2167 [CrossRef]
    [Google Scholar]
  43. Westphal O., Jann K. 1965; Bacterial lipopolysaccharide. Extraction with phenol-water and further applications of the procedure. In Methods in Carbohydrate Chemistry pp 83–91 Edited by Wistler R. New York: Academic Press;
    [Google Scholar]
  44. Xiang S.-H., Haase A. M., Reeves P. R. 1993; Variation of the rfb gene clusters in Salmonella enterica . J Bacteriol 175:4877–4884
    [Google Scholar]
  45. Ziolkowski A., Shashkov A. S., Swierzko A., Senchenkova S. N., Toukach F. V., Cedzynski M., Amano K.-I., Kaca W., Knirel Y. A. 1997; Structures of the O-antigens of Proteus bacilli belonging to OX group (serogroups O1–O3) used in Weil–Felix test. FEBS Lett 411:221–224 [CrossRef]
    [Google Scholar]
  46. Zubkov V. A., Nazarenko E. L., Gorshkova R. P., Ivanova E. P., Shashkov A. S., Knirel Y. A., Paramonov N. A., Ovodov Y. S. 1995; Structure of the capsular polysaccharide from Alteromonas sp . CMM 155. Carbohydr Res 275:147–154 [CrossRef]
    [Google Scholar]
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