1887

Abstract

The serotyping of O and H antigens is an important first step in the characterization of . However, serotyping has become increasingly technically demanding and expensive to perform. We have therefore sequenced additional O antigen gene clusters to provide information for the development of DNA-based serotyping methods. Three isolates had O antigen gene clusters with homology to the O123 O antigen region. O antigen clusters from two serogroup O58 strains had approximately 85 % identity with the O123 O antigen region over their entire length, suggesting that these and O antigen regions evolved from a common ancestor. The O antigen cluster of a serogroup O41 isolate had a lower level of identity with O123 over only part of its O antigen DNA cluster sequence, suggesting a different and more complex evolution of this gene cluster than those in the O58 strains. A large part of the O41 O antigen DNA cluster had very close identity with the O antigen cluster of an O62 strain. This region of DNA homology included the and genes. Therefore, molecular serotyping tests using only the O41 or O62 and genes would not differentiate between the two serogroups. The O123 O-antigenic polysaccharide and its repeating unit were characterized, and the chemical structure for O123 was entirely consistent with the O antigen gene cluster sequences of O123 and the O58 isolates. An understanding of both the genetic and structural composition of and O antigens is necessary for the development of novel molecular methods for serotyping these organisms.

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2009-07-01
2019-10-13
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Alignment of sequence differences between O antigen regions of isolates S897, S1669 and O123. Only nucleotide differences are shown. Numbers above the sequence designate the nucleotide number, eg. the first nucleotide shown is 28 in the common sequence, the last is nucleotide 15 805. [ PDF file] (19 KB)

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