1887

Abstract

Two isogenic sets of strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6 mutants with deletions in the gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the operon and subsequent complementation . It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence virulence or the average survival time of subcutaneously inoculated BALB/c naïve mice or animals immunized with this antigen.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.005678-0
2009-01-01
2024-11-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/1/26.html?itemId=/content/journal/jmm/10.1099/jmm.0.005678-0&mimeType=html&fmt=ahah

References

  1. Anisimov A. P. 1999; Molecular genetic mechanisms of the formation and functional significance of the capsule of Yersinia pestis . ScD thesis Russian Research Anti-Plague Institute ‘Microbe’; Saratov, Russia:
  2. Anisimov A. P. 2002a; Factors of Yersinia pestis providing circulation and persistence of plague pathogen in ecosystems of natural foci. Communication 2. Mol Gen Mikrobiol Virusol 4:3–11 (in Russian
    [Google Scholar]
  3. Anisimov A. P. 2002b; Yersinia pestis factors, assuring circulation and maintenance of the plague pathogen in natural foci ecosystems. Report 1 Mol Gen Mikrobiol Virusol 3:3–23 (in Russian
    [Google Scholar]
  4. Anisimov A. P., Lindler L. E., Pier G. B. 2004; Intraspecific diversity of Yersinia pestis . Clin Microbiol Rev 17:434–464 [CrossRef]
    [Google Scholar]
  5. Anisimov A. P., Panfertsev E. A., Svetoch T. E., Dentovskaya S. V. 2007; Variability of the protein sequences of LcrV between epidemic and atypical rhamnose-positive strains of Yersinia pestis . Adv Exp Med Biol 603:23–27
    [Google Scholar]
  6. Bakhteeva I. V., Kravchenko T. B., Titareva G. M., Ivanov S. A. 2007; Interaction of Yersinia pestis pH 6 antigen with various types of eukaryotic cells. The Problems of Particularly Dangerous Infections 94:40–42 (Saratov:
    [Google Scholar]
  7. Ben-Efraim S., Aronson M., Bichowsky-Slomnicki L. 1961; New antigenic component of Pasteurella pestis formed under specific conditions of pH and temperature. J Bacteriol 81:704–714
    [Google Scholar]
  8. Bengoechea J.-A., Lindner B., Seydel U., Díaz R., Moriyón I. 1998; Yersinia pseudotuberculosis and Yersinia pestis are more resistant to bactericidal cationic peptides than Yersinia enterocolitica . Microbiology 144:1509–1515 [CrossRef]
    [Google Scholar]
  9. Bichowsky-Slomnicki L., Ben-Efraim S. 1963; Biological activities in extracts of Pasteurella pestis and their relation to the “pH 6 antigen”. J Bacteriol 86:101–111
    [Google Scholar]
  10. Bowdler A. J. 2001 The Complete Spleen: Structure, Function, and Clinical Disorders Totowa, NJ: Humana;
    [Google Scholar]
  11. Brubaker R. R. 2006; Yersinia pestis and bubonic plague. In The Prokaryotes: a Handbook on the Biology of Bacteria. vol. 6, Proteobacteria: Gamma Subclass pp 399–442 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackelbrandt E. New York: Springer;
    [Google Scholar]
  12. Burrows T. W. 1957; Virulence of Pasteurella pestis . Nature 179:1246–1247 [CrossRef]
    [Google Scholar]
  13. Burrows T. W. 1963; Virulence of Pasteurella pestis and immunity to plague. Ergeb Mikrobiol Immunitatsforsch Exp Ther 37:59–113
    [Google Scholar]
  14. Cathelyn J. S., Crosby S. D., Lathem W. W., Goldman W. E., Miller V. L. 2006; RovA, a global regulator of Yersinia pestis , specifically required for bubonic plague. Proc Natl Acad Sci U S A 103:13514–13519 [CrossRef]
    [Google Scholar]
  15. Chandrika P., Kumanan K., Nachimuthu K. 1998; Comparison of three different techniques for the detection of duck plague virus antigen. Indian Vet J 75:843–844
    [Google Scholar]
  16. Chauvaux S., Rosso M. L., Frangeul L., Lacroix C., Labarre L., Schiavo A., Marceau M., Dillies M. A., Foulon J. other authors 2007; Transcriptome analysis of Yersinia pestis in human plasma: an approach for discovering bacterial genes involved in septicaemic plague. Microbiology 153:3112–3124 [CrossRef]
    [Google Scholar]
  17. Cherepanov P. A., Rosqvist R., Forsberg Å. 1998; Regulation of pH 6 antigen expression in Yersinia pestis . Med Microbiol 6: (Suppl. II), S8 (Ned N Voor)
    [Google Scholar]
  18. Conchas R. F., Carniel E. 1990; A highly efficient electroporation system for transformation of Yersinia . Gene 87:133–137 [CrossRef]
    [Google Scholar]
  19. Cornelis G. R. 2002; Yersinia type III secretion: send in the effectors. J Cell Biol 158:401–408 [CrossRef]
    [Google Scholar]
  20. Datsenko K. A., Wanner B. L. 2000; One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640–6645 [CrossRef]
    [Google Scholar]
  21. Domaradskiĭ I. V. 1987; The “side-effects” of plasmids (R-plasmids and virulence). Mol Gen Mikrobiol Virusol 6:3–9 (in Russian
    [Google Scholar]
  22. Donnenberg M. S., Kaper J. B. 1991; Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun 59:4310–4317
    [Google Scholar]
  23. Drozdov I. G., Anisimov A. P., Samoilova S. V., Yezhov I. N., Yeremin S. A., Karlyshev A. V., Krasilnikova V. M., Kravchenko V. I. 1995; Virulent non-capsulate Yersinia pestis variants constructed by insertion mutagenesis. J Med Microbiol 42:264–268 [CrossRef]
    [Google Scholar]
  24. Du Y., Rosqvist R., Forsberg Å. 2002; Role of fraction 1 antigen of Yersinia pestis in inhibition of phagocytosis. Infect Immun 70:1453–1460 [CrossRef]
    [Google Scholar]
  25. Felek S., Runco L. M., Thanassi D. G., Krukonis E. S. 2007; Characterization of six novel chaperone/usher systems in Yersinia pestis . Adv Exp Med Biol 603:97–105
    [Google Scholar]
  26. Finlay B. B., Falkow S. 1997; Common themes in microbial pathogenicity revisited. Microbiol Mol Biol Rev 61:136–169
    [Google Scholar]
  27. Finney D. J. 1978 Statistical Method in Biological Assay , 3rd edn. London: Hodder Arnold;
    [Google Scholar]
  28. Forman S., Wulff C. R., Myers-Morales T., Cowan C., Perry R. D., Straley S. C. 2008; yadBC of Yersinia pestis , a new virulence determinant for bubonic plague. Infect Immun 76:578–587 [CrossRef]
    [Google Scholar]
  29. Friedlander A. M., Welkos S. L., Worsham P. L., Andrews G. P., Heath D. G., Anderson G. W. Jr, Pitt M. L. M., Estep J., Davis K. 1995; Relationship between virulence and immunity as revealed in recent studies of the F1 capsule of Yersinia pestis . Clin Infect Dis 21 (Suppl. 2):S178–S181 [CrossRef]
    [Google Scholar]
  30. Gage K. L., Kosoy M. Y. 2005; Natural history of plague: perspectives from more than a century of research. Annu Rev Entomol 50:505–528 [CrossRef]
    [Google Scholar]
  31. Galván E. M., Chen H., Schifferli D. M. 2007; The Psa fimbriae of Yersinia pestis interact with phosphatidylcholine on alveolar epithelial cells and pulmonary surfactant. Infect Immun 75:1272–1279 [CrossRef]
    [Google Scholar]
  32. Gremyakova T. A. 2004; Structure-functional variability of Yersinia pestis antigens and methodology of construction of anti-plague immunoprophylactic preparations . ScD thesis G. N. Gabrichevskii Moscow Research Institute of Epidemiology and Microbiology; Moscow, Russia:
  33. Herrero M., de Lorenzo V., Timmis K. 1990; Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J Bacteriol 172:6557–6567
    [Google Scholar]
  34. Hohn B., Collins J. 1980; A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298 [CrossRef]
    [Google Scholar]
  35. Huang X.-Z., Lindler L. E. 2004; The pH 6 antigen is an antiphagocytic factor produced by Yersinia pestis independent of Yersinia outer proteins and capsule antigen. Infect Immun 72:7212–7219 [CrossRef]
    [Google Scholar]
  36. Hultgren S. J., Abraham S., Caparon M., Falk P., St Geme J. W. 3rd, Normark S. 1993; Pilus and nonpilus bacterial adhesins: assembly and function in cell recognition. Cell 73:887–901 [CrossRef]
    [Google Scholar]
  37. Iriarte M., Cornelis G. R. 1995; MyfF, an element of the network regulating the synthesis of fibrillae in Yersinia enterocolitica . J Bacteriol 177:738–744
    [Google Scholar]
  38. Isberg R. R. 1989a; Determinants for thermoinducible cell binding and plasmid-encoded cellular penetration detected in the absence of the Yersinia pseudotuberculosis invasin protein. Infect Immun 57:1998–2005
    [Google Scholar]
  39. Isberg R. R. 1989b; Mammalian cell adhesion functions and cellular penetration of enteropathogenic Yersinia species. Mol Microbiol 3:1449–1453 [CrossRef]
    [Google Scholar]
  40. Jarrett C. O., Sebbane F., Adamovicz J. J., Andrews G. P., Hinnebusch B. J. 2004; Flea-borne transmission model to evaluate vaccine efficacy against naturally acquired bubonic plague. Infect Immun 72:2052–2056 [CrossRef]
    [Google Scholar]
  41. Karlyshev A., Galyov E., Smirnov O., Abramov V., Zav'yalov V. P. 1994; Structure and regulation of a gene cluster involved in capsule formation of Yersinia pestis . pp 321–330 In Biological membranes: structure, biogenesis and dynamics (NATO ASI), Series H: Cell Biology, 82 Edited by op den Kamp J. A. F. Berlin: Springer;
    [Google Scholar]
  42. Knirel Y. A., Lindner B., Vinogradov E. V., Kocharova N. A., Senchenkova S. N., Shaikhutdinova R. Z., Dentovskaya S. V., Fursova N. K., Bakhteeva I. V. other authors 2005; Temperature-dependent variations and intraspecies diversity of the structure of the lipopolysaccharide of Yersinia pestis . Biochemistry 44:1731–1743 [CrossRef]
    [Google Scholar]
  43. Knirel Y. A., Dentovskaya S. V., Bystrova O. V., Kocharova N. A., Senchenkova S. N., Shaikhutdinova R. Z., Titareva G. M., Bakhteeva I. V., Lindner B. other authors 2007; Relationship of the lipopolysaccharide structure of Yersinia pestis to resistance to antimicrobial factors. Adv Exp Med Biol 603:88–96
    [Google Scholar]
  44. Kovalev O. A. 1978; Reaction types of regional redistribution of blood. Cor Vasa 20:230–239
    [Google Scholar]
  45. Li B., Zhou D., Wang Z., Song Z., Wang H., Li M., Dong X., Wu M., Guo Z., Yang R. 2007; Antibody profiling in plague patients by protein microarray. Microbes Infect 10:45–51
    [Google Scholar]
  46. Lindler L. E., Tall B. D. 1993; Yersinia pestis pH 6 antigen forms fimbriae and is induced by intracellular association with macrophages. Mol Microbiol 8:311–324 [CrossRef]
    [Google Scholar]
  47. Lindler L. E., Klempner M. S., Straley S. C. 1990; Yersinia pestis pH 6 antigen: genetic, biochemical, and virulence characterization. Infect Immun 58:2569–2577
    [Google Scholar]
  48. Liu F., Chen H., Galvan E. M., Lasaro M. A., Schifferli D. M. 2006; Effects of Psa and F1 on the adhesive and invasive interactions of Yersinia pestis with human respiratory tract epithelial cells. Infect Immun 74:5636–5644 [CrossRef]
    [Google Scholar]
  49. Liu H., Wang H., Qiu J., Wang X., Guo Z., Qiu Y., Zhou D., Han Y., Du Z. other authors 2008; Transcriptional profiling of a mice plague model: insights into interaction between Yersinia pestis and its host. JBasic Microbiol (in press). doi: 10.1002/jobm.200800027
    [Google Scholar]
  50. Lorange E. A., Race B. L., Sebbane F., Hinnebusch B. J. 2005; Poor vector competence of fleas and the evolution of hypervirulence in Yersinia pestis . J Infect Dis 191:1907–1912 [CrossRef]
    [Google Scholar]
  51. Makoveichuk E., Cherepanov P., Lundberg S., Forsberg Å., Olivecrona G. 2003; pH 6 antigen of Yersinia pestis interacts with plasma lipoproteins and cell membranes. J Lipid Res 44:320–330 [CrossRef]
    [Google Scholar]
  52. Marra A. 2004; Can virulence factors be viable antibacterial targets?. Expert Rev Anti Infect Ther 2:61–72 [CrossRef]
    [Google Scholar]
  53. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor: Cold Spring Harbor Laboratory;
    [Google Scholar]
  54. Ngo T. T., Leshoff H. M. 1985 Enzyme-Mediated Immunoassay New York: Plenum;
    [Google Scholar]
  55. Öuchterlony O. 1949; Antigen-antibody reaction in gels. Acta Pathol Microbiol Scand 26:507–515
    [Google Scholar]
  56. Oyston P. C., Karlyshev A. V., Wren B. W., Titball R. W. 2003; Signature-tagged mutagenesis of Yersinia pestis . Adv Exp Med Biol 529:39–41
    [Google Scholar]
  57. Panfertsev E. A., Cherepanov P. A., Karimova G. A. 1991; Construction of Yersinia pestis strains defective in pH6 synthesis. p. 22–24 In Proceedings of the XIV Scientific and Practical Conference on new technologies and biosystems: Achievements and perspectives Edited by Borovik R. V. Obolensk, USSR: Medbioekonomika Press;
    [Google Scholar]
  58. Parkhill J., Wren B. W., Thomson N. R., Titball R. W., Holden M. T., Prentice M. B., Sebaihia M., James K. D., Churcher C. other authors 2001; Genome sequence of Yersinia pestis , the causative agent of plague. Nature 413:523–527 [CrossRef]
    [Google Scholar]
  59. Payne D., Tatham D., Williamson E. D., Titball R. W. 1998; The pH 6 antigen of Yersinia pestis binds to β 1-linked galactosyl residues in glycosphingolipids. Infect Immun 66:4545–4548
    [Google Scholar]
  60. Perry R. D., Fetherston J. D. 1997; Yersinia pestis– etiologic agent of plague. Clin Microbiol Rev 10 35:123–66
    [Google Scholar]
  61. Porat R., McCabe W. R., Brubaker R. R. 1995; Lipopolysaccharide-associated resistance to killing of yersiniae by complement. J Endotoxin Res 2:91–97
    [Google Scholar]
  62. Prentki P., Karch F., Iida S., Meyer J. 1981; The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication. Gene 14:289–299 [CrossRef]
    [Google Scholar]
  63. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  64. Sha J., Agar S. L., Baze W. B., Olano J. P., Fadl A. A., Erova T. E., Wang S., Foltz S. M., Suarez G. other authors 2008; Braun lipoprotein (Lpp) contributes to virulence of yersiniae: potential role of Lpp in inducing bubonic and pneumonic plague. Infect Immun 76:1390–1409 [CrossRef]
    [Google Scholar]
  65. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Biotechnology (N.Y.) 1:784–791 [CrossRef]
    [Google Scholar]
  66. Stepanshina V. N., Gremiakova T. A., Anisimov A. P., Potapov V. D. 1993; The physicochemical and biological characteristics of the Yersinia pestis pH 6 antigen isolated by an immunosorption method. Zh Mikrobiol Epidemiol Immunobiol 3:12–17 (in Russian
    [Google Scholar]
  67. Sundin C., Wolfgang M. C., Lory S., Forsberg Å., Frithz-Lindsten E. 2002; Type IV pili are not specifically required for contact dependent translocation of exoenzymes by Pseudomonas aeruginosa . Microb Pathog 33:265–277 [CrossRef]
    [Google Scholar]
  68. Thanassi D. G., Saulino E. T., Hultgren S. J. 1998; The chaperone/usher pathway: a major terminal branch of the general secretory pathway. Curr Opin Microbiol 1:223–231 [CrossRef]
    [Google Scholar]
  69. Titball R. W., Williamson E. D. 2001; Vaccination against bubonic and pneumonic plague. Vaccine 19:4175–4184 [CrossRef]
    [Google Scholar]
  70. Vodop'ianov S. O., Mishan'kin B. N. 1985; Adhesion pili in Yersinia pestis . Zh Mikrobiol Epidemiol Immunobiol 6:13–17 (in Russian
    [Google Scholar]
  71. Vodop'ianov S. O., Popova G. O., Vasil'eva G. I., Mishan'kin B. N. 1990; The phenomenon of pilus formation in the interaction of Yersinia pestis with macrophages in experimental animals. Zh Mikrobiol Epidemiol Immunobiol 3:3–6 (in Russian
    [Google Scholar]
  72. Vodop'ianov S. O., Atarova G. T., Oleinikov I. P., Saiamov S. R., Orlova G. M., Mishan'kin B. N. 1993; The fibronectin-binding capacity of Yersinia pestis . Zh Mikrobiol Epidemiol Immunobiol 3:6–12 (in Russian
    [Google Scholar]
  73. Vodop'ianov S. O., Rybyanets A. A., Verkina L. M., Romanova L. V., Sorokina T. B., Mishan'kin B. N. 1995; The protective activity of the adhesion pili of Yersinia pestis . Zh Mikrobiol Epidemiol Immunobiol 5:26–29 (in Russian
    [Google Scholar]
  74. Yang Y., Merriam J. J., Mueller J. P., Isberg R. R. 1996; The psa locus is responsible for thermoinducible binding of Yersinia pseudotuberculosis to cultured cells. Infect Immun 64:2483–2489
    [Google Scholar]
  75. Zav'yalov V. P., Abramov V. M., Cherepanov P. A., Spirina G. V., Chernovskaya T. V., Vasiliev A. M., Zav'yalova G. A. 1996; pH 6 antigen (PsaA protein) of Yersinia pestis , a novel bacterial Fc-receptor. FEMS Immunol Med Microbiol 14:53–57 [CrossRef]
    [Google Scholar]
  76. Zhou D., Qin L., Han Y., Qiu J., Chen Z., Li B., Song Y., Wang J., Guo Z. other authors 2006; Global analysis of iron assimilation and Fur regulation in Yersinia pestis . FEMS Microbiol Lett 258:9–17 [CrossRef]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.005678-0
Loading
/content/journal/jmm/10.1099/jmm.0.005678-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error