1887

Abstract

Two isogenic sets of strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6 mutants with deletions in the gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the operon and subsequent complementation . It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence virulence or the average survival time of subcutaneously inoculated BALB/c naïve mice or animals immunized with this antigen.

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2009-01-01
2021-10-19
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