1887

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Volume 72, Issue 5

Genomic features and virulence characteristics of a rare strain causing human infection No Access

Abstract

is a clinically rare opportunistic pathogen in the genus , and the genomic features and virulence characteristics of strains that cause human infection remain unclear.

strains with different virulence induce different host innate immune responses .

. This work aimed to understand the sequence diversity, phylogenetic relationship, and virulence of BPM causing human infection.

The comparative molecular and genomic analyses, and mouse infection studies were applied to analyse the virulence and genomic features of BPM originating from China.

The whole genome sequence analysis showed that the genomes of BPM and other avirulent strains were broadly similar, comprising two highly syntenic chromosomes with comparable numbers of coding regions (CDs), protein family distributions, and horizontally acquired genomic islands. By examining species-specific genomic regions, we obtained molecular explanations for previously known differences in virulence and discovered the potential specific virulence-associated genes of BPM, which likely work together to confer the virulence of BPM. Significantly reduced LD and survival rates during mouse infection experiments were found in BPM compared to the avirulent E264 (BtE264).

Taken together, the results of this study provide basic information on the genomic features and virulence characteristics of the virulent strain BPM, which is helpful for understanding its evolution as it relates to pathogenesis and environmental adaptability.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): B. thailandensisBPM , comparative genomics analysis , genomic features , virulence characteristics and whole-genome sequencing
Funding
This study was supported by the:
  • the Youth Project of the National Natural Science Foundation of China (Award 82002113)
    • Principle Award Recipient: JinLi
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 82072348)
    • Principle Award Recipient: Wei-PingLu
© 2023 The Authors
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/content/journal/jmm/10.1099/jmm.0.001688
2023-05-17
2024-05-05
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Genomic features and virulence characteristics of a rare Burkholderia thailandensis strain causing human infection
J. Med. Microbiol. 72, 001688 (2023); https://doi.org/10.1099/jmm.0.001688
/content/journal/jmm/10.1099/jmm.0.001688
/content/journal/jmm/10.1099/jmm.0.001688
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